ID   MCM33_MAIZE             Reviewed;         768 AA.
AC   Q9SX03;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=DNA replication licensing factor MCM3 homolog 3;
DE            EC=3.6.4.12;
DE   AltName: Full=Replication origin activator 3;
DE            Short=ROA-3;
GN   Name=ROA3;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. LG11; TISSUE=Root;
RA   Sabelli P.A., Parker J.S., Barlow P.W.;
RT   "cDNA and promoter sequences for MCM3 homologues from maize, and protein
RT   localization in cycling cells.";
RL   J. Exp. Bot. 50:1315-1322(1999).
CC   -!- FUNCTION: Acts as a factor that allows the DNA to undergo a single
CC       round of replication per cell cycle. Required for DNA replication and
CC       cell proliferation (By similarity). May act as a component of the MCM
CC       complex which is the putative replicative helicase of the replication
CC       licensing system in eukaryotic cells. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
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DR   EMBL; AF073331; AAD48087.1; -; mRNA.
DR   AlphaFoldDB; Q9SX03; -.
DR   SMR; Q9SX03; -.
DR   PRIDE; Q9SX03; -.
DR   MaizeGDB; 131629; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; Q9SX03; baseline and differential.
DR   GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006271; P:DNA strand elongation involved in DNA replication; IBA:GO_Central.
DR   GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR   GO; GO:1902975; P:mitotic DNA replication initiation; IBA:GO_Central.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR031327; MCM.
DR   InterPro; IPR008046; Mcm3.
DR   InterPro; IPR018525; MCM_CS.
DR   InterPro; IPR001208; MCM_dom.
DR   InterPro; IPR041562; MCM_lid.
DR   InterPro; IPR027925; MCM_N.
DR   InterPro; IPR033762; MCM_OB.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11630; PTHR11630; 1.
DR   Pfam; PF00493; MCM; 1.
DR   Pfam; PF17855; MCM_lid; 1.
DR   Pfam; PF14551; MCM_N; 1.
DR   Pfam; PF17207; MCM_OB; 1.
DR   PRINTS; PR01657; MCMFAMILY.
DR   PRINTS; PR01659; MCMPROTEIN3.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00350; MCM; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00847; MCM_1; 1.
DR   PROSITE; PS50051; MCM_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..768
FT                   /note="DNA replication licensing factor MCM3 homolog 3"
FT                   /id="PRO_0000292981"
FT   DOMAIN          290..497
FT                   /note="MCM"
FT   REGION          661..690
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           472..475
FT                   /note="Arginine finger"
FT   BINDING         340..347
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   768 AA;  85209 MW;  B5EB8658687B64F4 CRC64;
     MEINEEAMAA HKRAFLDFLD QDVGKGVYMQ AVRDMVQNKR HRLIIGMDDL RNHNLDLARR
     VIRTPGEYMQ PASDAVSEVA RNLDPKFLKE GERVMVGFSG PFGFHRVTPR DLMSSFIGTM
     VCVEGIVTKC SLVRPKVVKS VHFCPVTGDF LSREYRDITS FVGLPTGSVY PTRDDNGNLL
     VTEYGMCEYK DHQTLSMQEV PENSAPGQLP RTVDVIVEDD LVDCCKPGDR VSIVGVYKAL
     PGKSKGSVSG VFRTVLIANN VSLLNKEANA PVYTREDLKR MKEISRRNDT FDLLGNSLAP
     SIYGHLWIKK AVVLLMLGGV EKNLKNGTHL RGDINMMMVG DPSVAKSQLL RAVMNIAPLA
     ISTTGRGSSG VGLTAAVTSD QETGERRLEA GAMVLADRGV VCIDEFDKMN DQDRVAIHEV
     MEQQTVTIAK AGIHASLNAR CSVIAAANPI YGTYDRSLTP TKNIGLPDSL LSRFDLLFIV
     LDQMDPEIDR QISEHVARMH RYCTDDGGAR SLDKEGYAEE DDGDANAAIF VKYDRMLHGQ
     DRRRGKKSKQ DRLTVKFLKK YIHYAKNLIQ PRLTDEASDH IATSYAELRD GSANAKSGGG
     TLPITARTLE TIIRLSTAHA KMKLRHEVLK SDVEAALQVL NFAIYHKELT EMEEREQREM
     EMKQQADHDA GATGGTVDGH GSSGNDPMDV DVGSNDQNVS AERIQAFEAL LGQHVLANHI
     DQMSIDEIEQ MVNRESTAPY TRSQVEFILE RMQDANRVMI RDGVVRII