ID   MCM3_ENTHI              Reviewed;         597 AA.
AC   Q24849;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=DNA replication licensing factor MCM3;
DE            EC=3.6.4.12;
GN   Name=MCM3;
OS   Entamoeba histolytica.
OC   Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC   Entamoeba.
OX   NCBI_TaxID=5759;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 30459 / HM-1:IMSS;
RA   Gangopadhyay S.S., Lohia A.;
RL   Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Acts as a factor that allows the DNA to undergo a single
CC       round of replication per cell cycle. Required for DNA replication and
CC       cell proliferation (By similarity). May act as a component of the MCM
CC       complex which is the putative replicative helicase of the replication
CC       licensing system in eukaryotic cells. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X98048; CAA66661.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q24849; -.
DR   SMR; Q24849; -.
DR   STRING; 5759.rna_EHI_103600-1; -.
DR   VEuPathDB; AmoebaDB:EHI5A_077240; -.
DR   VEuPathDB; AmoebaDB:EHI7A_062040; -.
DR   VEuPathDB; AmoebaDB:EHI8A_049600; -.
DR   VEuPathDB; AmoebaDB:EHI_103600; -.
DR   VEuPathDB; AmoebaDB:KM1_109070; -.
DR   eggNOG; KOG0479; Eukaryota.
DR   GO; GO:0042555; C:MCM complex; IEA:InterPro.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR031327; MCM.
DR   InterPro; IPR008046; Mcm3.
DR   InterPro; IPR018525; MCM_CS.
DR   InterPro; IPR001208; MCM_dom.
DR   InterPro; IPR041562; MCM_lid.
DR   InterPro; IPR033762; MCM_OB.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11630; PTHR11630; 1.
DR   Pfam; PF00493; MCM; 1.
DR   Pfam; PF17855; MCM_lid; 1.
DR   Pfam; PF17207; MCM_OB; 1.
DR   PRINTS; PR01657; MCMFAMILY.
DR   PRINTS; PR01659; MCMPROTEIN3.
DR   SMART; SM00350; MCM; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00847; MCM_1; 1.
DR   PROSITE; PS50051; MCM_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW   Nucleotide-binding; Nucleus.
FT   CHAIN           1..597
FT                   /note="DNA replication licensing factor MCM3"
FT                   /id="PRO_0000194096"
FT   DOMAIN          180..386
FT                   /note="MCM"
FT   MOTIF           361..364
FT                   /note="Arginine finger"
FT   BINDING         229..236
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   597 AA;  66413 MW;  129D9BB171ED6566 CRC64;
     MEGHIHHITP RNITASILQQ KVAVQGIITK SSQIRPLLQT AVQFCPLDYS THARDLSHAD
     VMVKLSSKTP DGKPLELEPG LSTYKDFQTL VVQEMPESAP TGQMPRSVIV ILLDQLVDKG
     KPGDRVIING TLKALAGPNH SSTFKVVLEA ENINTLQSEG PELTEIDKEN IKKVMKEENP
     INLFSKSIAP SIYGHSDVKK AILLMLVGAT PKIRLRSRVR GDIHVMLCGD PSTAKSQLLR
     YVMSIAPLAV STNGRGATGV GLTAAVVNDP DTNQRTLEAG AMVLADRGIC CVDEFDKMSI
     EDRAAMHEVM EQQTVTVQKA GIHTGIKCKM SILAAANPSN GNYDFKKSPM ENLYFPESLL
     SRFDLIFIIL DSSTEELDRK LSQHVLKMHR HFDALTEQRG DDEVNVLALV DAEREKIGDA
     PVYQDSLCMK EKNYLLINSL KNMLLMLEIS PTPSLSESAS ETIADAYVKL RENERLKRIK
     HNFKIKTLPI TARALDSLIR LAEAHARIRG SDTIDEIDAQ VAVQLILMKI WEGNITTHIA
     RKVREYLTNE LIAECKDVIQ FDDILSICGI DKPTLMKILP QLSFGYDEDE EFVYKQN