MCM4_ORYSJ
ID MCM4_ORYSJ Reviewed; 862 AA.
AC Q5JKB0; A0A0P0V3T1;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=DNA replication licensing factor MCM4;
DE EC=3.6.4.12;
DE AltName: Full=Minichromosome maintenance protein 4;
DE Short=OsMCM4;
GN Name=MCM4; OrderedLocusNames=Os01g0544450, LOC_Os01g36390;
GN ORFNames=OSJNBa0051H17.26;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP GENE FAMILY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Probable component of the MCM2-7 complex (MCM complex) that
CC may function as a DNA helicase and which is essential to undergo a
CC single round of replication initiation and elongation per cell cycle in
CC eukaryotic cells. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the minichromosome maintenance (MCM) complex, a
CC heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD88098.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP004232; BAD88098.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; BAS72587.1; -; Genomic_DNA.
DR RefSeq; XP_015630179.1; XM_015774693.1.
DR AlphaFoldDB; Q5JKB0; -.
DR SMR; Q5JKB0; -.
DR STRING; 4530.OS01T0544450-01; -.
DR PaxDb; Q5JKB0; -.
DR PRIDE; Q5JKB0; -.
DR EnsemblPlants; Os01t0544450-01; Os01t0544450-01; Os01g0544450.
DR GeneID; 107275673; -.
DR Gramene; Os01t0544450-01; Os01t0544450-01; Os01g0544450.
DR KEGG; osa:107275673; -.
DR eggNOG; KOG0478; Eukaryota.
DR HOGENOM; CLU_000995_7_2_1; -.
DR InParanoid; Q5JKB0; -.
DR OMA; KNTIRIC; -.
DR OrthoDB; 266497at2759; -.
DR PlantReactome; R-OSA-9640882; Assembly of pre-replication complex.
DR PlantReactome; R-OSA-9645850; Activation of pre-replication complex.
DR PlantReactome; R-OSA-9675824; DNA replication Initiation.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR Genevisible; Q5JKB0; OS.
DR GO; GO:0000785; C:chromatin; IEA:EnsemblPlants.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000347; C:THO complex; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006271; P:DNA strand elongation involved in DNA replication; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IEA:EnsemblPlants.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008047; MCM_4.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR PANTHER; PTHR11630:SF66; PTHR11630:SF66; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01660; MCMPROTEIN4.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..862
FT /note="DNA replication licensing factor MCM4"
FT /id="PRO_0000425992"
FT DOMAIN 453..659
FT /note="MCM"
FT ZN_FING 289..317
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 1..132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 635..638
FT /note="Arginine finger"
FT COMPBIAS 25..41
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..107
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 503..510
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 862 AA; 94905 MW; B3FDA885D82E9FDE CRC64;
MASRGGGGGG DGNSPPPSVS SPDVRPSSPL PATNSSPPQS GRRGGGRRRR GSASPYPSSP
SLGGFETPPH PGRRTPSGGA AARQQRQNWT GGRFPPTPST PMSTDDVPLS SEAGDEDTPE
TDGGGGGGAG ADATPVFVWG TNISVQDVNA AILRFLRHFR DPRDAGRVDP VMDEGKYMRA
IHRILELEGG ESLDVNAHDV FDHDPDLYGK MVRYPLEVLA IFDIVLMDLV ARIEPLFEKH
IQTRIYNLKS SVCLRNLNPS DIEKMVSIKG MIIRCSSVIP ELKEAVFRCL VCGFYSEPVM
VDRGRVTEPH ICQKEQCKAT NSMTLVHNRC RFADKQIIKL QETPDEIPEG GTPHTVSVLM
HDKLVDAGKP GDRVEITGIY RAMSIRVGPT QRTVKSIFKT YIDCLHIKKT DKSRLHVEDS
METDNPNANK TTEDDFLRDK VEKLKELSKL PDIYDRLTRS LAPNIWELDD VKRGLLCQLF
GGNALRLPSG ASFRGDINIL LVGDPGTSKS QLLQYMHKLS PRGIYTSGRG SSAVGLTAYV
TKDPETGETV LESGALVLSD KGVCCIDEFD KMSDNARSML HEVMEQQTVS IAKAGIIASL
NARTSVLACA NPTESRYNPR LSVIDNIHLP PTLLSRFDLI YLILDKADEQ TDRRLAKHIV
SLHFENPNIE ELEVLDLPTL VAYISYARKH IQPQLSDEAA EELTRGYVEM RKRGNSPGSR
KKVITATARQ IESLIRLSEA LARMRFSEMV EVQDVVEAFR LLEVAMQQSA TDHATGTIDM
DLIMTGISAS ERQRRDNLVA ATRNLVMEKM QLGGPSVRMI ELLEEIRKQS SMEVHLHDLR
GALGTLMTEG AVVIHGDSVK RV
