MCM5_ARATH
ID MCM5_ARATH Reviewed; 727 AA.
AC O80786;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=DNA replication licensing factor MCM5;
DE EC=3.6.4.12;
DE AltName: Full=Minichromosome maintenance protein 5;
DE Short=AtMCM5;
GN Name=MCM5; OrderedLocusNames=At2g07690; ORFNames=T12J2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
RN [5]
RP SUBUNIT, INTERACTION WITH ETG1, AND SUBCELLULAR LOCATION.
RX PubMed=18528439; DOI=10.1038/emboj.2008.107;
RA Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G.,
RA Witters E., Inze D., De Veylder L.;
RT "The DNA replication checkpoint aids survival of plants deficient in the
RT novel replisome factor ETG1.";
RL EMBO J. 27:1840-1851(2008).
RN [6]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19357199; DOI=10.1104/pp.109.136614;
RA Shultz R.W., Lee T.J., Allen G.C., Thompson W.F., Hanley-Bowdoin L.;
RT "Dynamic localization of the DNA replication proteins MCM5 and MCM7 in
RT plants.";
RL Plant Physiol. 150:658-669(2009).
CC -!- FUNCTION: Probable component of the MCM2-7 complex (MCM complex) that
CC may function as a DNA helicase and which is essential to undergo a
CC single round of replication initiation and elongation per cell cycle in
CC eukaryotic cells. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the minichromosome maintenance (MCM) complex, a
CC heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7.
CC Interacts with EGT1. {ECO:0000269|PubMed:18528439}.
CC -!- INTERACTION:
CC O80786; Q501D5: ETG1; NbExp=5; IntAct=EBI-2131365, EBI-2131354;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Localized in the nucleus
CC during G1, S and G2 phases of the cell cycle, and released into the
CC cytoplasmic compartment during mitosis.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=O80786-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in shoot apex and flower buds.
CC {ECO:0000269|PubMed:19357199}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX820823; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AC004483; AAC26671.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06128.1; -; Genomic_DNA.
DR EMBL; BX820823; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G84487; G84487.
DR RefSeq; NP_178812.1; NM_126771.6. [O80786-1]
DR AlphaFoldDB; O80786; -.
DR SMR; O80786; -.
DR BioGRID; 804; 12.
DR IntAct; O80786; 2.
DR MINT; O80786; -.
DR STRING; 3702.AT2G07690.1; -.
DR PaxDb; O80786; -.
DR PRIDE; O80786; -.
DR ProteomicsDB; 238242; -. [O80786-1]
DR EnsemblPlants; AT2G07690.1; AT2G07690.1; AT2G07690. [O80786-1]
DR GeneID; 815415; -.
DR Gramene; AT2G07690.1; AT2G07690.1; AT2G07690. [O80786-1]
DR KEGG; ath:AT2G07690; -.
DR Araport; AT2G07690; -.
DR TAIR; locus:2054698; AT2G07690.
DR eggNOG; KOG0481; Eukaryota.
DR InParanoid; O80786; -.
DR OMA; ITYCKTR; -.
DR PhylomeDB; O80786; -.
DR PRO; PR:O80786; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80786; baseline and differential.
DR Genevisible; O80786; AT.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0000347; C:THO complex; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; TAS:TAIR.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008048; MCM5.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01661; MCMPROTEIN5.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Cell cycle; Cytoplasm; DNA replication;
KW DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..727
FT /note="DNA replication licensing factor MCM5"
FT /id="PRO_0000425993"
FT DOMAIN 325..531
FT /note="MCM"
FT MOTIF 507..510
FT /note="Arginine finger"
FT BINDING 375..382
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 727 AA; 81015 MW; 81F519BDBD73CFB3 CRC64;
MSGWDEGAVY YSDQPQFPEA GDAATISPHA VLTKFKEFIR NFEIEQNCFP YREALLDNPK
RLVVHLEDLL SFDSDLPSLI RSAPADYLPV FEKAAGEVLT GLKMREANEG GVMEEPLTRD
VQILLTSRED PVSMRLLGAQ YISKLVKISG ISIAASRVKA KATYVFLVCK NCKKTREVPC
RPGLGGAIVP RSCDNIPQPG EEPCPLDPWM VVPDRSQYVD QQTLKLQENP EDVPTGELPR
NMLLSVDRHL VQTIVPGTRL TVMGIYSIFQ ASSSSNSHKG AVAIRQPYIR VVGLEDTNEA
SSRGPANFTP DEEEEFKKFA DSQDVYKNIC TKIAPSIFGH EDVKRAAACL LFGGSRKSLP
DGVKLRGDIN VLLLGDPSTA KSQFLKFVEK TAPIAVYTSG KGSSAAGLTA SVIRDSSTRE
FYLEGGAMVL ADGGVVCIDE FDKMRPEDRV AIHEAMEQQT ISIAKAGITT VLNSRTSVLA
AANPPSGRYD DLKTAQDNID LQTTILSRFD LIFIVKDIRK YSQDKEIASH IIRVHASANK
FSDENTDSKE DNWLKRYIQY CRARCHPRLS KDAAENLQRK YVTIRMDMKR RAHETGEAAP
IPITVRQLEA IVRLSESLAK MRLSHEATPD DVDKAFKLFD TSTMDAARSG INQQINITGE
MANEIKQAET QIKRRMGIGA RLSERRLIED LARMGMNDSM VRRALLIMHQ RGEVEYQRER
RSIVRKA
