MCM5_DICDI
ID MCM5_DICDI Reviewed; 757 AA.
AC Q54CP4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 105.
DE RecName: Full=DNA replication licensing factor mcm5;
DE EC=3.6.4.12;
GN Name=mcm5; ORFNames=DDB_G0292958;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Acts as component of the MCM2-7 complex (MCM complex) which
CC is the putative replicative helicase essential for 'once per cell
CC cycle' DNA replication initiation and elongation in eukaryotic cells.
CC The active ATPase sites in the MCM2-7 ring are formed through the
CC interaction surfaces of two neighboring subunits such that a critical
CC structure of a conserved arginine finger motif is provided in trans
CC relative to the ATP-binding site of the Walker A box of the adjacent
CC subunit (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the MCM2-7 complex. The complex forms a toroidal
CC hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-
CC MCM3-MCM5 (By simililarity).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P33992}.
CC Cytoplasm, cytosol {ECO:0000250|UniProtKB:P33992}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
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DR EMBL; AAFI02000197; EAL61028.1; -; Genomic_DNA.
DR RefSeq; XP_629372.1; XM_629370.1.
DR AlphaFoldDB; Q54CP4; -.
DR SMR; Q54CP4; -.
DR STRING; 44689.DDB0232354; -.
DR PaxDb; Q54CP4; -.
DR EnsemblProtists; EAL61028; EAL61028; DDB_G0292958.
DR GeneID; 8628890; -.
DR KEGG; ddi:DDB_G0292958; -.
DR dictyBase; DDB_G0292958; mcm5.
DR eggNOG; KOG0481; Eukaryota.
DR HOGENOM; CLU_000995_7_2_1; -.
DR InParanoid; Q54CP4; -.
DR OMA; ITYCKTR; -.
DR PhylomeDB; Q54CP4; -.
DR Reactome; R-DDI-68867; Assembly of the pre-replicative complex.
DR Reactome; R-DDI-68949; Orc1 removal from chromatin.
DR Reactome; R-DDI-68962; Activation of the pre-replicative complex.
DR Reactome; R-DDI-69052; Switching of origins to a post-replicative state.
DR PRO; PR:Q54CP4; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0042555; C:MCM complex; ISS:dictyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008048; MCM5.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01661; MCMPROTEIN5.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell cycle; Cytoplasm; DNA replication; DNA-binding; Helicase;
KW Hydrolase; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..757
FT /note="DNA replication licensing factor mcm5"
FT /id="PRO_0000328207"
FT DOMAIN 319..523
FT /note="MCM"
FT /evidence="ECO:0000255"
FT REGION 606..625
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 499..502
FT /note="Arginine finger"
SQ SEQUENCE 757 AA; 84221 MW; A5EE685333F86BA2 CRC64;
MSGFDEGNVT VSGGGGKGGF KKNNGFVEDT SVRDLFKRFI NEWKDQDNVF IYKEQLRQHY
NLGWHYIEVS IDHLTDFNQE LSGRFISSPN ELMPSFEDAI KDIIKEMNYN KEQVDEDIQI
LFKSSANPEP IRYLRAGLIS KLVKVQGIVI SASRTQPKPS TMVVKCKNCQ HTQTLHIRPG
IVSSVLPQQC ERGSNDAGKP CPNNPYVVLS DQSTFVNQQI LKLQESPETI PTGEMPRHII
LSLDKSLADK ITPGTRIKVL GVLGIFEGGG KRREIAGGTI RTNYLRVLGI TSDNAGRDSM
HFTPSEEQSF KVFSRRQDLR NIIASSIAPS IYGHEDIKRA ISCQLFGGSS KKLPDKMRLR
GDINLLLLGD PGTAKSQLLK FVEKVAPISV YTSGKGSSAA GLTASVIREP STGEYYLEGG
AMVVADGGVV CIDEFDKMNV DDRVAIHEAM EQQTISIAKA GITTILNSRT SVLAAANPVY
GRYNDAADDN INFQSTILSR FDLIFIVKDP KNEKRDFIIS KHVINIHEKS SRSGGSGSVG
NNTYDLSNTV VDDSHIGENE VTIQYLKKYI AYARSRISPR LSEDAVTTLK NHYVSVRAKS
KEQEMINNGS YGGGGSKNSV ETERKKRKNA IPITVRQLEA IIRISESLAK MSLSPIATNE
HAKEAIRLFD ISTFDAITTN NTVNETLTPE RLENIRTAEK YLKDRVPIGS SIRIKDVRFQ
LSRSGLDHFT ILKAVDILVG RDEFEFRNQK RTLFRKQ
