MCM5_ORYSJ
ID MCM5_ORYSJ Reviewed; 729 AA.
AC Q6KAJ4; A0A0P0VQS3;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=DNA replication licensing factor MCM5;
DE EC=3.6.4.12;
DE AltName: Full=Minichromosome maintenance protein 5;
DE Short=OsMCM5;
GN Name=MCM5; OrderedLocusNames=Os02g0797400, LOC_Os02g55410;
GN ORFNames=OJ1004_E04.32, OsJ_08729;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Probable component of the MCM2-7 complex (MCM complex) that
CC may function as a DNA helicase and which is essential to undergo a
CC single round of replication initiation and elongation per cell cycle in
CC eukaryotic cells. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the minichromosome maintenance (MCM) complex, a
CC heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
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DR EMBL; AP003975; BAD19092.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF10310.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS81375.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57980.1; -; Genomic_DNA.
DR EMBL; AK073980; BAG93743.1; -; mRNA.
DR RefSeq; XP_015623388.1; XM_015767902.1.
DR AlphaFoldDB; Q6KAJ4; -.
DR SMR; Q6KAJ4; -.
DR STRING; 4530.OS02T0797400-01; -.
DR PaxDb; Q6KAJ4; -.
DR PRIDE; Q6KAJ4; -.
DR EnsemblPlants; Os02t0797400-01; Os02t0797400-01; Os02g0797400.
DR GeneID; 4331016; -.
DR Gramene; Os02t0797400-01; Os02t0797400-01; Os02g0797400.
DR KEGG; osa:4331016; -.
DR eggNOG; KOG0481; Eukaryota.
DR HOGENOM; CLU_000995_7_2_1; -.
DR InParanoid; Q6KAJ4; -.
DR OMA; ITYCKTR; -.
DR OrthoDB; 266497at2759; -.
DR PlantReactome; R-OSA-9640882; Assembly of pre-replication complex.
DR PlantReactome; R-OSA-9645850; Activation of pre-replication complex.
DR PlantReactome; R-OSA-9675824; DNA replication Initiation.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6KAJ4; OS.
DR GO; GO:0000785; C:chromatin; IEA:EnsemblPlants.
DR GO; GO:0005737; C:cytoplasm; IEA:EnsemblPlants.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000347; C:THO complex; IEA:EnsemblPlants.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003688; F:DNA replication origin binding; IEA:InterPro.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008048; MCM5.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01661; MCMPROTEIN5.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..729
FT /note="DNA replication licensing factor MCM5"
FT /id="PRO_0000425994"
FT DOMAIN 326..532
FT /note="MCM"
FT MOTIF 508..511
FT /note="Arginine finger"
FT BINDING 376..383
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 729 AA; 80376 MW; 3B49DDA3F13D6FBC CRC64;
MSGWDEGAVF YSDQAQFPRG GPGGDPSADL TRHSALRKFK EFLRGFTGPT GDFPYRESLV
HNRDHVTVAI EDLDAFDAEL SDKIRKSPAD YLPLFETAAS EVLASLRSKV AGETGEMEEP
ATGDVQIFLS SKENCLSMRS IGADYMSKLV KIAGITIAAS RVKAKATHVT LLCKNCRSVK
TVPCRPGLGG AIVPRSCDHV PQPGEEPCPL DPWIAVPDKS KYVDLQTLKL QENPEDVPTG
ELPRNMLLSV DRHLVQTIVP GTRLTVIGIY SVYQASANQK GAVGVKQPYI RVVGLEQSRD
ANSNGPSNFT LDEEMEFKEF AQRPDAYVKI CSMIGPSIYG HSDVKKAIAC LLFGGSKKRL
PDGVRLRGDI HVLLLGDPST AKSQFLKFVE KTAPIAVYTS GKGSSAAGLT ASVIRDGSSR
EFYLEGGAMV LADGGVVCID EFDKMRPEDR VAIHEAMEQQ TISIAKAGIT TVLNSRTSVL
AAANPIAGRY DDLKTAQDNI DLQTTILSRF DLIFIVKDVR MYDQDKRIAS HIIKVHASGA
AASSKNTDAS EGENWLKRYI EYCRVTCKPR LSEKAAEMLQ NKYVEIRQKM RQQAHETGRA
AAIPITVRQL EAIIRLSESL AKMRLTSVAT PEHVEEAFRL FNVSTVDAAR SGINEHLNLS
PDIANEIKQA EAQIKRRMGI GSHISERRLI DELNRMGMNE SIVRRALLIM HQRDEVEYKR
ERHVIVRKA
