MCM6_ARATH
ID MCM6_ARATH Reviewed; 831 AA.
AC F4KAB8;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=DNA replication licensing factor MCM6;
DE EC=3.6.4.12;
DE AltName: Full=Minichromosome maintenance protein 6;
DE Short=AtMCM6;
GN Name=MCM6; OrderedLocusNames=At5g44635; ORFNames=K15C23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
RN [4]
RP SUBUNIT, AND INTERACTION WITH ETG1.
RX PubMed=18528439; DOI=10.1038/emboj.2008.107;
RA Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G.,
RA Witters E., Inze D., De Veylder L.;
RT "The DNA replication checkpoint aids survival of plants deficient in the
RT novel replisome factor ETG1.";
RL EMBO J. 27:1840-1851(2008).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=19357199; DOI=10.1104/pp.109.136614;
RA Shultz R.W., Lee T.J., Allen G.C., Thompson W.F., Hanley-Bowdoin L.;
RT "Dynamic localization of the DNA replication proteins MCM5 and MCM7 in
RT plants.";
RL Plant Physiol. 150:658-669(2009).
CC -!- FUNCTION: Probable component of the MCM2-7 complex (MCM complex) that
CC may function as a DNA helicase and which is essential to undergo a
CC single round of replication initiation and elongation per cell cycle in
CC eukaryotic cells. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC -!- SUBUNIT: Component of the minichromosome maintenance (MCM) complex, a
CC heterotetramer composed of MCM2, MCM3, MCM4, MCM5, MCM6 and MCM7.
CC Interacts with ETG1. {ECO:0000269|PubMed:18528439}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apex and flower buds.
CC {ECO:0000269|PubMed:19357199}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB024024; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED95141.1; -; Genomic_DNA.
DR RefSeq; NP_680393.1; NM_148088.2.
DR AlphaFoldDB; F4KAB8; -.
DR SMR; F4KAB8; -.
DR BioGRID; 19742; 16.
DR IntAct; F4KAB8; 14.
DR MINT; F4KAB8; -.
DR STRING; 3702.AT5G44635.1; -.
DR PaxDb; F4KAB8; -.
DR PRIDE; F4KAB8; -.
DR ProteomicsDB; 250827; -.
DR EnsemblPlants; AT5G44635.1; AT5G44635.1; AT5G44635.
DR GeneID; 834492; -.
DR Gramene; AT5G44635.1; AT5G44635.1; AT5G44635.
DR KEGG; ath:AT5G44635; -.
DR Araport; AT5G44635; -.
DR TAIR; locus:504954997; AT5G44635.
DR eggNOG; KOG0480; Eukaryota.
DR HOGENOM; CLU_000995_3_2_1; -.
DR InParanoid; F4KAB8; -.
DR OMA; KYYIAQI; -.
DR PhylomeDB; F4KAB8; -.
DR PRO; PR:F4KAB8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KAB8; baseline and differential.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000347; C:THO complex; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IEA:InterPro.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IBA:GO_Central.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IBA:GO_Central.
DR GO; GO:1902969; P:mitotic DNA replication; IBA:GO_Central.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR008049; MCM6.
DR InterPro; IPR041024; Mcm6_C.
DR InterPro; IPR018525; MCM_CS.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR027925; MCM_N.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF18263; MCM6_C; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF14551; MCM_N; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR PRINTS; PR01662; MCMPROTEIN6.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00847; MCM_1; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell cycle; DNA replication; DNA-binding; Helicase; Hydrolase;
KW Metal-binding; Nucleotide-binding; Nucleus; Reference proteome; Zinc;
KW Zinc-finger.
FT CHAIN 1..831
FT /note="DNA replication licensing factor MCM6"
FT /id="PRO_0000425996"
FT DOMAIN 345..551
FT /note="MCM"
FT ZN_FING 155..182
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 258..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 666..705
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 527..530
FT /note="Arginine finger"
FT COMPBIAS 671..688
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 395..402
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 831 AA; 92851 MW; 310467E0D92DEB79 CRC64;
MEAFGGFVMD EQAIQVENVF LEFLKSFRLD ANKPELYYEA EIEAIRGGES TMMYIDFSHV
MGFNDALQKA IADEYLRFEP YLRNACKRFV IEMNPSFISD DTPNKDINVS FYNLPFTKRL
RELTTAEIGK LVSVTGVVTR TSEVRPELLY GTFKCLDCGS VIKNVEQQFK YTQPTICVSP
TCLNRARWAL LRQESKFADW QRVRMQETSK EIPAGSLPRS LDVILRHEIV EQARAGDTVI
FTGTVVVIPD ISALAAPGER AECRRDSSQQ KSSTAGHEGV QGLKALGVRD LSYRLAFIAN
SVQIADGSRN TDMRNRQNDS NEDDQQQFTA EELDEIQQMR NTPDYFNKLV GSMAPTVFGH
QDIKRAVLLM LLGGVHKTTH EGINLRGDIN VCIVGDPSCA KSQFLKYTAG IVPRSVYTSG
KSSSAAGLTA TVAKEPETGE FCIEAGALML ADNGICCIDE FDKMDIKDQV AIHEAMEQQT
ISITKAGIQA TLNARTSILA AANPVGGRYD KSKPLKYNVN LPPAILSRFD LVYVMIDDPD
EVTDYHIAHH IVRVHQKHEA ALSPEFTTVQ LKRYIAYAKT LKPKLSPEAR KLLVESYVAL
RRGDTTPGTR VAYRMTVRQL EALIRLSEAI ARSHLEILVK PSHVLLAVRL LKTSVISVES
GDIDLSEYQD ANGDNMDDTD DIENPVDGEE DQQNGAAEPA SATADNGAAA QKLVISEEEY
DRITQALVIR LRQHEETVNK DSSELPGIRQ KELIRWFIDQ QNEKKKYSSQ EQVKLDIKKL
RAIIESLVCK EGHLIVLANE QEEAAEAEET KKKSSQRDER ILAVAPNYVI E
