ID   MCM8_ORYSJ              Reviewed;         789 AA.
AC   B9FKM7; Q60EQ1;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Probable DNA helicase MCM8;
DE            EC=3.6.4.12;
DE   AltName: Full=Minichromosome maintenance 8;
DE            Short=OsMCM8;
GN   Name=MCM8; OrderedLocusNames=Os05g0464100, LOC_Os05g38850;
GN   ORFNames=OJ1280_A04.12, OsJ_18839;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [7]
RP   GENE FAMILY.
RX   PubMed=17556508; DOI=10.1104/pp.107.101105;
RA   Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT   "Genome-wide analysis of the core DNA replication machinery in the higher
RT   plants Arabidopsis and rice.";
RL   Plant Physiol. 144:1697-1714(2007).
CC   -!- FUNCTION: Probable DNA helicase that may play a role in DNA repair
CC       during meiosis. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAU90181.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAF17691.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=BAG98258.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR   EMBL; AC108500; AAU90181.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP008211; BAF17691.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AP014961; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM000142; EEE64010.1; -; Genomic_DNA.
DR   EMBL; AK108033; BAG98258.1; ALT_SEQ; mRNA.
DR   RefSeq; XP_015638234.1; XM_015782748.1.
DR   AlphaFoldDB; B9FKM7; -.
DR   SMR; B9FKM7; -.
DR   STRING; 4530.OS05T0464100-01; -.
DR   PaxDb; B9FKM7; -.
DR   PRIDE; B9FKM7; -.
DR   GeneID; 4339038; -.
DR   KEGG; osa:4339038; -.
DR   eggNOG; KOG0480; Eukaryota.
DR   HOGENOM; CLU_000995_1_0_1; -.
DR   OrthoDB; 266497at2759; -.
DR   Proteomes; UP000000763; Chromosome 5.
DR   Proteomes; UP000007752; Chromosome 5.
DR   Proteomes; UP000059680; Chromosome 5.
DR   Genevisible; B9FKM7; OS.
DR   GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR031327; MCM.
DR   InterPro; IPR018525; MCM_CS.
DR   InterPro; IPR001208; MCM_dom.
DR   InterPro; IPR041562; MCM_lid.
DR   InterPro; IPR033762; MCM_OB.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11630; PTHR11630; 1.
DR   Pfam; PF00493; MCM; 1.
DR   Pfam; PF17855; MCM_lid; 1.
DR   Pfam; PF17207; MCM_OB; 1.
DR   PRINTS; PR01657; MCMFAMILY.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00350; MCM; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00847; MCM_1; 1.
DR   PROSITE; PS50051; MCM_2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; DNA damage; DNA repair; DNA-binding; Helicase; Hydrolase;
KW   Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome;
KW   Zinc; Zinc-finger.
FT   CHAIN           1..789
FT                   /note="Probable DNA helicase MCM8"
FT                   /id="PRO_0000426002"
FT   DOMAIN          339..546
FT                   /note="MCM"
FT   ZN_FING         174..201
FT                   /note="C4-type"
FT                   /evidence="ECO:0000255"
FT   MOTIF           522..525
FT                   /note="Arginine finger"
FT   BINDING         391..398
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   789 AA;  86271 MW;  7A05ED32B0CCEF61 CRC64;
     MYDDGPRKGK PGGLSMDAAT AAGLAAVWPE YFPEESEFAA DGRSARLAAD LVDLFSSPDA
     SDLLSRVEDD GDILSLPVDF QQLSNLTWIT EALQENPKEA LLSMGAAVHL IVCASRDLQL
     GDINKINIRL YNHTKTIALK NLKAAYIKKL VTVRGTVLKV STVKPLVLQL NFQCMKCATK
     FPRVFCDGKF SPPVSCSIQG CKSRTFIPMR STAKLMDFQK IRIQELASGE SHEEGRVPRT
     IECELTEDLV DCCIPGETVT VTGIVKVLNN YMDVGGGKSK SRNQGLYYLY LEAISVRNSK
     VHAASGNSDA ASGSFGFQAF TEKDLEFISK FKEEHGADVF RQILHSFCPS IYGHELVKAG
     ITLALFGGVQ KHSIDQNKVP VRGDIHAVVV GDPGLGKSQL LQAAAAVSPR GIYVCGNTTT
     NAGLTVAVVK DSMSNDYAFE AGAMVLADRG ICCIDEFDKM SAEHQALLEA MEQQCVSVAK
     AGLVASLSAR TSVLAAANPV GGHYDRAKTV NENLKMSAAL LSRFDLVFIL LDKPDELLDK
     RVSDHIIALH SNDGGPFTAN KRIRTVPQFN ASTEFGVGRT SLASRLRLHP EKDKDFCPLP
     GPLLRKYISY ARSHVNPRIS MPSPAADSLQ KFYLDLRKQS DSADGTPITA RQLESLVRLA
     EARARVDLRE EVTLEDAKEV IDIMTESLYD KCVDEHGVVD FARSGGMSNQ KQSKKFLRAL
     NEQCDLQKKD CFSMNEMYNL ADRISLQVAN LDAIVESLNN AGYITKKGSS MYQVVTSSYQ
     GSQATWSRR