MCMBP_ARATH
ID MCMBP_ARATH Reviewed; 589 AA.
AC Q501D5; O22880; Q0WST4;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Mini-chromosome maintenance complex-binding protein;
DE Short=MCM-BP;
DE Short=MCM-binding protein;
DE AltName: Full=Protein E2F TARGET GENE 1;
GN Name=ETG1; OrderedLocusNames=At2g40550; ORFNames=T2P4.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION.
RX PubMed=16126853; DOI=10.1104/pp.105.066290;
RA Vandepoele K., Vlieghe K., Florquin K., Hennig L., Beemster G.T.S.,
RA Gruissem W., Van de Peer Y., Inze D., De Veylder L.;
RT "Genome-wide identification of potential plant E2F target genes.";
RL Plant Physiol. 139:316-328(2005).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, INDUCTION BY E2FA AND
RP E2FB, AND INTERACTION WITH THE MCM COMPLEX.
RX PubMed=18528439; DOI=10.1038/emboj.2008.107;
RA Takahashi N., Lammens T., Boudolf V., Maes S., Yoshizumi T., De Jaeger G.,
RA Witters E., Inze D., De Veylder L.;
RT "The DNA replication checkpoint aids survival of plants deficient in the
RT novel replisome factor ETG1.";
RL EMBO J. 27:1840-1851(2008).
RN [7]
RP FUNCTION.
RX PubMed=20090939; DOI=10.1371/journal.pgen.1000817;
RA Takahashi N., Quimbaya M., Schubert V., Lammens T., Vandepoele K.,
RA Schubert I., Matsui M., Inze D., Berx G., De Veylder L.;
RT "The MCM-binding protein ETG1 aids sister chromatid cohesion required for
RT postreplicative homologous recombination repair.";
RL PLoS Genet. 6:E1000817-E1000817(2010).
CC -!- FUNCTION: Associated component of the MCM complex that acts as a
CC regulator of DNA replication. Binds to the MCM complex during late S
CC phase and may act by promoting the disassembly of the MCM complex from
CC chromatin. Required for sister chromatid cohesion.
CC {ECO:0000269|PubMed:18528439, ECO:0000269|PubMed:20090939}.
CC -!- SUBUNIT: Interacts with the MCM complex. {ECO:0000269|PubMed:18528439}.
CC -!- INTERACTION:
CC Q501D5; O80786: MCM5; NbExp=5; IntAct=EBI-2131354, EBI-2131365;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18528439}.
CC Note=Associates with the replisome complex.
CC -!- INDUCTION: Expression is regulated by E2FA and E2FB.
CC {ECO:0000269|PubMed:16126853, ECO:0000269|PubMed:18528439}.
CC -!- DISRUPTION PHENOTYPE: Serrated leaves due to cell cycle inhibition and
CC to a stringent late G2 cell cycle arrest. The leaf blade area is almost
CC normal while the average abaxial pavement cell area increases
CC significantly in the mutant plants, accompanied with a decrease in cell
CC number per leaf. At the bolting stage, younger leaves display a
CC slightly elongated and serrated leaf phenotype. In addition, the root
CC growth rate of the mutant plants is significantly reduced.
CC {ECO:0000269|PubMed:18528439}.
CC -!- SIMILARITY: Belongs to the MCMBP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB87582.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002336; AAB87582.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09847.1; -; Genomic_DNA.
DR EMBL; BT022032; AAY25444.1; -; mRNA.
DR EMBL; AK227836; BAE99814.1; -; mRNA.
DR PIR; H84830; H84830.
DR RefSeq; NP_181587.2; NM_129617.5.
DR AlphaFoldDB; Q501D5; -.
DR BioGRID; 3988; 14.
DR IntAct; Q501D5; 14.
DR MINT; Q501D5; -.
DR STRING; 3702.AT2G40550.1; -.
DR PaxDb; Q501D5; -.
DR PRIDE; Q501D5; -.
DR ProteomicsDB; 238702; -.
DR EnsemblPlants; AT2G40550.1; AT2G40550.1; AT2G40550.
DR GeneID; 818650; -.
DR Gramene; AT2G40550.1; AT2G40550.1; AT2G40550.
DR KEGG; ath:AT2G40550; -.
DR Araport; AT2G40550; -.
DR TAIR; locus:2061833; AT2G40550.
DR eggNOG; KOG2545; Eukaryota.
DR HOGENOM; CLU_029811_0_0_1; -.
DR InParanoid; Q501D5; -.
DR OMA; HTEMIQQ; -.
DR OrthoDB; 1011739at2759; -.
DR PhylomeDB; Q501D5; -.
DR PRO; PR:Q501D5; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q501D5; baseline and differential.
DR Genevisible; Q501D5; AT.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0042555; C:MCM complex; TAS:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IMP:TAIR.
DR GO; GO:0006261; P:DNA-templated DNA replication; IBA:GO_Central.
DR GO; GO:0006301; P:postreplication repair; IMP:TAIR.
DR GO; GO:0007062; P:sister chromatid cohesion; IMP:TAIR.
DR InterPro; IPR019140; MCM_complex-bd.
DR PANTHER; PTHR13489; PTHR13489; 1.
DR Pfam; PF09739; MCM_bind; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; DNA replication; Mitosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..589
FT /note="Mini-chromosome maintenance complex-binding protein"
FT /id="PRO_0000405814"
FT REGION 163..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 193..211
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 249
FT /note="V -> I (in Ref. 4; BAE99814)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 589 AA; 65755 MW; 05B98D03B992931E CRC64;
MGGPAYDCLT NPLGAVRFSF VNALSSGYDP ASSVGKDWGV VDLFRHYFSD ESAISQVPIL
DSSSIKWVQP KTLVRFRGMI QDMLGNEFYA GAYKDDSTWR TNKYSDVSQF PEGSSTEIQV
WERRLLYCVP VPGKNQWTEC SSQELKNRFL DLTGQNREKR VRVDEEMTDS MDSSTLEAGR
NGSPFKKMKV GEATSSASES QVPQTSGIPP ATSADSLPCL VKIYDSPESD LKLNDVVEFL
GVLTFDPIVM MDTDTLDENS DALSEAESVQ MPSGKVPRLH CLIHRKLETQ HFLHGSSLLP
EPKSPQIFKE IRESLMKYLT GLLGNDHIAA QFLLLHLLSK VHGRVDNVAV GKLSLNLIHL
NKESMSIFGT QLSGALKSLL PFTQSIPLTI EYLNTASFGP KKDYGINRLM PGVLQIADGT
HLILDETELQ PGTLNSVGVE NANLLKNLLE CQKVEYDFQY YKMEMATDVQ MLIFSEGKSN
IMPADLVLPL QPSQVNSLEV ITPETAETWR CYLATCKSLS HSIGQELQQV VENDLVAARQ
TDRSLGSQDL SRLLTMARMM SVSYGETTLS LEHWQMVLEL ERLRKERLK
