MCMR_DROME
ID MCMR_DROME Reviewed; 885 AA.
AC Q9VF30;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=DNA replication licensing factor REC;
DE AltName: Full=Protein mini chromosome maintenance-related;
DE AltName: Full=Protein recombination-defective;
GN Name=rec; ORFNames=CG31293;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, VARIANTS HIS-118 AND ALA-422, AND
RP MUTAGENESIS OF SER-455.
RC STRAIN=Berkeley, and Canton-S; TISSUE=Ovary;
RX PubMed=14676427; DOI=10.1266/ggs.78.363;
RA Matsubayashi H., Yamamoto M.-T.;
RT "REC, a new member of the MCM-related protein family, is required for
RT meiotic recombination in Drosophila.";
RL Genes Genet. Syst. 78:363-371(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Required for meiotic DNA recombination in females. Probably
CC not involved in DNA repair and recombination in somatic cells.
CC {ECO:0000269|PubMed:14676427}.
CC -!- INTERACTION:
CC Q9VF30; Q86B50: mei-217; NbExp=2; IntAct=EBI-99278, EBI-15133128;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCM family. {ECO:0000305}.
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DR EMBL; AB120941; BAC92698.1; -; mRNA.
DR EMBL; AB120942; BAC92699.1; -; mRNA.
DR EMBL; AB120943; BAC92700.1; -; mRNA.
DR EMBL; AB120944; BAC92701.1; -; Genomic_DNA.
DR EMBL; AB120945; BAC92702.1; -; Genomic_DNA.
DR EMBL; AB120946; BAC92703.1; -; Genomic_DNA.
DR EMBL; AB120947; BAC92704.1; -; Genomic_DNA.
DR EMBL; AB120948; BAC92705.1; -; Genomic_DNA.
DR EMBL; AB120949; BAC92706.1; -; Genomic_DNA.
DR EMBL; AB120950; BAC92707.1; -; Genomic_DNA.
DR EMBL; AB120951; BAC92708.1; -; mRNA.
DR EMBL; AB120952; BAC92709.1; -; Genomic_DNA.
DR EMBL; AB120953; BAC92710.1; -; mRNA.
DR EMBL; AE014297; AAF55231.2; -; Genomic_DNA.
DR RefSeq; NP_001262615.1; NM_001275686.1.
DR RefSeq; NP_732072.1; NM_169674.2.
DR AlphaFoldDB; Q9VF30; -.
DR SMR; Q9VF30; -.
DR BioGRID; 72108; 4.
DR DIP; DIP-61892N; -.
DR IntAct; Q9VF30; 4.
DR STRING; 7227.FBpp0082647; -.
DR PaxDb; Q9VF30; -.
DR EnsemblMetazoa; FBtr0083193; FBpp0082647; FBgn0003227.
DR EnsemblMetazoa; FBtr0334595; FBpp0306662; FBgn0003227.
DR GeneID; 49241; -.
DR KEGG; dme:Dmel_CG31293; -.
DR CTD; 49241; -.
DR FlyBase; FBgn0003227; rec.
DR VEuPathDB; VectorBase:FBgn0003227; -.
DR eggNOG; KOG0478; Eukaryota.
DR GeneTree; ENSGT01050000244972; -.
DR HOGENOM; CLU_000995_7_2_1; -.
DR InParanoid; Q9VF30; -.
DR OMA; VYGHELP; -.
DR OrthoDB; 266497at2759; -.
DR PhylomeDB; Q9VF30; -.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-DME-68949; Orc1 removal from chromatin.
DR Reactome; R-DME-68962; Activation of the pre-replicative complex.
DR SignaLink; Q9VF30; -.
DR BioGRID-ORCS; 49241; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 49241; -.
DR PRO; PR:Q9VF30; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0003227; Expressed in antenna and 15 other tissues.
DR ExpressionAtlas; Q9VF30; baseline and differential.
DR Genevisible; Q9VF30; DM.
DR GO; GO:0042555; C:MCM complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0008094; F:ATP-dependent activity, acting on DNA; TAS:UniProtKB.
DR GO; GO:0003678; F:DNA helicase activity; TAS:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; TAS:UniProtKB.
DR GO; GO:0007131; P:reciprocal meiotic recombination; IMP:UniProtKB.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR031327; MCM.
DR InterPro; IPR001208; MCM_dom.
DR InterPro; IPR041562; MCM_lid.
DR InterPro; IPR033762; MCM_OB.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11630; PTHR11630; 1.
DR Pfam; PF00493; MCM; 1.
DR Pfam; PF17855; MCM_lid; 1.
DR Pfam; PF17207; MCM_OB; 1.
DR PRINTS; PR01657; MCMFAMILY.
DR SMART; SM00350; MCM; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50051; MCM_2; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell cycle; Cell division; DNA recombination; DNA-binding;
KW Meiosis; Metal-binding; Nucleotide-binding; Nucleus; Reference proteome;
KW Zinc; Zinc-finger.
FT CHAIN 1..885
FT /note="DNA replication licensing factor REC"
FT /id="PRO_0000194127"
FT DOMAIN 430..627
FT /note="MCM"
FT ZN_FING 281..308
FT /note="C4-type"
FT /evidence="ECO:0000255"
FT REGION 36..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 796..818
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 473..480
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VARIANT 118
FT /note="Y -> H"
FT /evidence="ECO:0000269|PubMed:14676427"
FT VARIANT 422
FT /note="V -> A"
FT /evidence="ECO:0000269|PubMed:14676427"
FT MUTAGEN 455
FT /note="S->F: In rec3; temperature-sensitive allele.
FT Recombination-defective."
FT /evidence="ECO:0000269|PubMed:14676427"
SQ SEQUENCE 885 AA; 97585 MW; 9CDC8D6CF91C21C6 CRC64;
MNPTLSRGGA PSRFVRGRGR GGGAYRPYFY FRRNGRVIPA GGNRQPNQGE PGAPDAPSVP
PATRQPRGWS RTAGKRGRNA HLDCSFLRPE NYAAPEDGLQ VQSIAVDNPR AYSGWRLYFL
REKYEEGNEL ATRIMAVEAY YQRNPHTFDF VMIRDRGFFP LCAAGIKSDA QLKEVWPSLS
EDIREHPLRT LGTLSLAMHT VVVNHQLDCN DSTTAMVSTP EQYVLPTPRV RKIYARPDDF
VPVESIEGIS HSRVDTLFSI RGFVSNVGEP SYSLTWQAFR CSRCQMEIAM RQRGTFQPRP
YQCKRSECVA RDEFVPLRSS PYTRLSIRQI IRVEESSLNL VHDFETSMPA EMDVELRHDL
VDAVRVGQEV VVTGILKLQE LGDDTTTGDT SNQMQPYLKA VSIRDASSIK REFSERDLEA
IVMINAEPNS FKLLVQSIAP EVYGHELPKA ACLLSLLGGK GAETEAINVL LVGDPGIGKT
KILQSCAQIA ERGAHVSGKR GAQSAQQLGV TFAGRNKRVL QAGSLMMASG GGHCTLDDVD
KLASKQAVLL QCLQSEEVNL PLAGAFASFP AQPSVIACAN PQRGQYDEGR YLLQNINISP
SLLREFHLVY ILLDKPSERD MSLTAHVRAL HAGARKRARI AARYALKPKM SDSMCEVSLN
VPAAGKDDDT IKTEDDNDSI MQQDYDLDKR LEVLPEEGDL DLLPPILIKK FLSYARQELN
PVLNEDASNA VLRYFLELKG SCNLDEDVSS QIGAGQLLAI IHLSQARARL DLSHVVSPQH
VRDVIALLTE SITQTSLKEG SSRQGTRGGG GAGGGAGKSA QLRNFLELTK RRSAALGRRI
FEFDELKEIG TRAGILTGFS QLVEMANLGG YLLMKGANMY EVVPD
