ID   MCNI_ECOLX              Reviewed;          93 AA.
AC   Q46970; C3VUZ4;
DT   04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Microcin N immunity protein {ECO:0000303|PubMed:20979348};
DE   AltName: Full=Microcin-24 immunity protein {ECO:0000303|Ref.1};
GN   Name=mcnI {ECO:0000303|PubMed:20979348}; Synonyms=mtfI {ECO:0000303|Ref.1};
OS   Escherichia coli.
OG   Plasmid p24-2 {ECO:0000303|PubMed:8058821}.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2424;
RA   O'Brien G.J., Mahanty H.K.;
RT   "Complete nucleotide sequence of microcin 24 genetic region and analysis of
RT   a new ABC transporter.";
RL   Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND NOMENCLATURE.
RC   STRAIN=2424;
RX   PubMed=20979348; DOI=10.1111/j.1574-6968.2010.02106.x;
RA   Corsini G., Karahanian E., Tello M., Fernandez K., Rivero D.,
RA   Saavedra J.M., Ferrer A.;
RT   "Purification and characterization of the antimicrobial peptide microcin
RT   N.";
RL   FEMS Microbiol. Lett. 312:119-125(2010).
RN   [3]
RP   IDENTIFICATION, AND FUNCTION.
RC   STRAIN=2424; PLASMID=p24-2;
RX   PubMed=8058821; DOI=10.1006/plas.1994.1030;
RA   O'Brien G.J., Mahanty H.K.;
RT   "Colicin 24, a new plasmid-borne colicin from a uropathogenic strain of
RT   Escherichia coli.";
RL   Plasmid 31:288-296(1994).
CC   -!- FUNCTION: Probably able to protect the producing cell against microcin
CC       N (microcin 24). {ECO:0000305|PubMed:8058821}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MceB microcin immunity protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACP43449.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U47048; AAA88771.1; -; Genomic_DNA.
DR   EMBL; FJ895580; ACP43449.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q46970; -.
DR   SMR; Q46970; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030153; P:bacteriocin immunity; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Bacteriocin immunity; Cell inner membrane; Cell membrane; Membrane;
KW   Plasmid; Transmembrane; Transmembrane helix.
FT   CHAIN           1..93
FT                   /note="Microcin N immunity protein"
FT                   /id="PRO_0000218714"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   93 AA;  11426 MW;  6B0AEB65DA9BA635 CRC64;
     MSFLNFAFSP VFFSIMACYF IVWRNKRNEF VCNRLLSIII ISFLICFIYP WLNYKIEVKY
     YIFEQFYLFC FLSSLVAVVI NLIVYFILYR RCI