MCNN_ECOLX
ID MCNN_ECOLX Reviewed; 89 AA.
AC Q46971; C3VUZ5;
DT 04-MAY-2001, integrated into UniProtKB/Swiss-Prot.
DT 29-SEP-2021, sequence version 2.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=Microcin N {ECO:0000303|PubMed:20979348, ECO:0000303|PubMed:27190283};
DE AltName: Full=Colicin 24 {ECO:0000303|PubMed:8058821};
DE AltName: Full=Microcin-24 {ECO:0000303|Ref.1};
DE Short=Mcc24 {ECO:0000303|Ref.1};
DE Flags: Precursor;
GN Name=mcnN {ECO:0000303|PubMed:20979348}; Synonyms=mtfS {ECO:0000303|Ref.1};
OS Escherichia coli.
OG Plasmid p24-2 {ECO:0000303|PubMed:8058821}.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=2424;
RA O'Brien G.J., Mahanty H.K.;
RT "Complete nucleotide sequence of microcin 24 genetic region and analysis of
RT a new ABC transporter.";
RL Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, NOMENCLATURE,
RP BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, INDUCTION, AND MASS
RP SPECTROMETRY.
RC STRAIN=2424;
RX PubMed=20979348; DOI=10.1111/j.1574-6968.2010.02106.x;
RA Corsini G., Karahanian E., Tello M., Fernandez K., Rivero D.,
RA Saavedra J.M., Ferrer A.;
RT "Purification and characterization of the antimicrobial peptide microcin
RT N.";
RL FEMS Microbiol. Lett. 312:119-125(2010).
RN [3]
RP PROTEIN SEQUENCE OF 16-23, FUNCTION, SUBCELLULAR LOCATION, AND MASS
RP SPECTROMETRY.
RC STRAIN=2424;
RX PubMed=27190283; DOI=10.1093/femsle/fnw095;
RA Kaur K., Tarassova O., Dangeti R.V., Azmi S., Wishart D., McMullen L.,
RA Stiles M.;
RT "Characterization of a highly potent antimicrobial peptide microcin N from
RT uropathogenic Escherichia coli.";
RL FEMS Microbiol. Lett. 363:0-0(2016).
RN [4]
RP IDENTIFICATION, AND FUNCTION.
RC STRAIN=2424; PLASMID=p24-2;
RX PubMed=8058821; DOI=10.1006/plas.1994.1030;
RA O'Brien G.J., Mahanty H.K.;
RT "Colicin 24, a new plasmid-borne colicin from a uropathogenic strain of
RT Escherichia coli.";
RL Plasmid 31:288-296(1994).
RN [5]
RP FUNCTION, AND BIOTECHNOLOGY.
RC STRAIN=2424;
RX PubMed=10396637; DOI=10.2307/1592614;
RA Wooley R.E., Gibbs P.S., Shotts E.B. Jr.;
RT "Inhibition of Salmonella typhimurium in the chicken intestinal tract by a
RT transformed avirulent avian Escherichia coli.";
RL Avian Dis. 43:245-250(1999).
RN [6]
RP FUNCTION, AND BIOTECHNOLOGY.
RC STRAIN=2424;
RX PubMed=11560267; DOI=10.2460/ajvr.2001.62.1399;
RA Wooley R.E., Ritchie B.W., Currin M.F., Chitwood S.W., Sanchez S.,
RA Crane M.M., Lamberski N.;
RT "In vitro inhibition of Salmonella organisms isolated from reptiles by an
RT inactivated culture of microcin-producing Escherichia coli.";
RL Am. J. Vet. Res. 62:1399-1401(2001).
CC -!- FUNCTION: Active against E.coli and Salmonella, but not Listeria or
CC Campylobacter (PubMed:8058821, PubMed:27190283, PubMed:10396637).
CC Channel-forming microcin (By similarity). Probably neutralized by its
CC immunity protein McnI (Probable). {ECO:0000250|UniProtKB:Q9Z4N4,
CC ECO:0000269|PubMed:10396637, ECO:0000269|PubMed:27190283,
CC ECO:0000269|PubMed:8058821, ECO:0000305|PubMed:8058821}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Stable on heating to 80 degrees Celsius for 30 minutes, unstable at
CC 100 degrees Celsius and to autoclaving.
CC {ECO:0000269|PubMed:20979348};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20979348,
CC ECO:0000269|PubMed:27190283}.
CC -!- INDUCTION: Induced during exponential growth in all media tested;
CC activity remains in stationary phase, although expression varies
CC depending on the growth medium (at protein level).
CC {ECO:0000269|PubMed:20979348}.
CC -!- PTM: Mass spectrometry suggests 3 of the 4 Met residues of the mature
CC peptide are oxidized. {ECO:0000269|PubMed:27190283}.
CC -!- MASS SPECTROMETRY: Mass=7274.23; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:20979348};
CC -!- MASS SPECTROMETRY: Mass=7224.0; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:27190283};
CC -!- BIOTECHNOLOGY: When avirulent E.coli transformed with a plasmid
CC encoding the genes necessary for microcin production, secretion and
CC immunity is fed continuously to broiler chickens it significantly
CC reduces the S.typhimurium load in the intestinal tract
CC (PubMed:10396637). 44 of 57 reptile-derived Salmonella strains were
CC sensitive in in vitro killing assays to the above strain, suggesting it
CC might be used to reduce pet-acquired Salmonellosis (PubMed:11560267).
CC {ECO:0000269|PubMed:10396637, ECO:0000269|PubMed:11560267}.
CC -!- SIMILARITY: Belongs to the class IIa microcin family.
CC {ECO:0000305|PubMed:20979348}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA88772.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U47048; AAA88772.1; ALT_INIT; Genomic_DNA.
DR EMBL; FJ895580; ACP43450.1; -; Genomic_DNA.
DR AlphaFoldDB; Q46971; -.
DR SMR; Q46971; -.
DR TCDB; 1.C.58.1.2; the microcin e492/c24 (microcin e492) family.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing; Plasmid;
KW Secreted; Signal; Virulence.
FT SIGNAL 1..15
FT /evidence="ECO:0000269|PubMed:27190283"
FT CHAIN 16..89
FT /note="Microcin N"
FT /id="PRO_0000005678"
FT CONFLICT 38..47
FT /note="AAFGARGGLG -> PPLVPERFR (in Ref. 1; AAA88772)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 89 AA; 8896 MW; D9E8A67B7CC38EAE CRC64;
MRELDREELN CVGGAGDPLA DPNSQIVRQI MSNAAWGAAF GARGGLGGMA VGAAGGVTQT
VLQGAAAHMP VNVPIPKVPM GPSWNGSKG
