ID   MCP1A_MACPC             Reviewed;         169 AA.
AC   B9V973;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Macrocypin-1a;
OS   Macrolepiota procera (Parasol mushroom).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Agaricaceae; Macrolepiota.
OX   NCBI_TaxID=56183;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN
RP   SEQUENCE OF 2-46, AND FUNCTION.
RC   TISSUE=Fruiting body;
RX   PubMed=19678836; DOI=10.1111/j.1742-4658.2009.07138.x;
RA   Sabotic J., Popovic T., Puizdar V., Brzin J.;
RT   "Macrocypins, a family of cysteine protease inhibitors from the
RT   basidiomycete Macrolepiota procera.";
RL   FEBS J. 276:4334-4345(2009).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS), AND MUTAGENESIS OF GLY-25.
RX   PubMed=19846555; DOI=10.1074/jbc.m109.043331;
RA   Renko M., Sabotic J., Mihelic M., Brzin J., Kos J., Turk D.;
RT   "Versatile loops in mycocypins inhibit three protease families.";
RL   J. Biol. Chem. 285:308-316(2010).
CC   -!- FUNCTION: Inhibits papain and cysteine cathepsin endopeptidases, and
CC       also inhibits cathepsins B and H, which exhibit both exopeptidase and
CC       endopeptidase activities. {ECO:0000269|PubMed:19678836}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I85 family.
CC       {ECO:0000305}.
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DR   EMBL; FJ495239; ACL99723.1; -; mRNA.
DR   EMBL; FJ495240; ACL99724.1; -; Genomic_DNA.
DR   PDB; 3H6Q; X-ray; 1.64 A; A=1-169.
DR   PDBsum; 3H6Q; -.
DR   AlphaFoldDB; B9V973; -.
DR   SMR; B9V973; -.
DR   MEROPS; I85.001; -.
DR   GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Protease inhibitor;
KW   Thiol protease inhibitor.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:19678836"
FT   CHAIN           2..169
FT                   /note="Macrocypin-1a"
FT                   /id="PRO_0000397842"
FT   MUTAGEN         25
FT                   /note="G->A: 20-fold lower inhibition."
FT                   /evidence="ECO:0000269|PubMed:19846555"
FT   CONFLICT        3
FT                   /note="F -> L (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        7
FT                   /note="F -> L (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        11
FT                   /note="L -> R (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        14
FT                   /note="A -> V (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        18
FT                   /note="H -> P (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        21..22
FT                   /note="Missing (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        45
FT                   /note="G -> P (in Ref. 1; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   STRAND          6..13
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          28..30
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          40..42
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          52..56
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   HELIX           58..60
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          65..69
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          79..82
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          92..94
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   HELIX           96..102
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          108..112
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          120..128
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          131..140
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          143..151
FT                   /evidence="ECO:0007829|PDB:3H6Q"
FT   STRAND          161..167
FT                   /evidence="ECO:0007829|PDB:3H6Q"
SQ   SEQUENCE   169 AA;  19194 MW;  F6500168477E4957 CRC64;
     MGFEDGFYTI LHLAEGQHPN SKIPGGMYAS SKDGKDVPVT AEPLGPQSKI RWWIARDPQA
     GDDMYTITEF RIDNSIPGQW SRSPVETEVP VYLYDRIKAE ETGYTCAWRI QPADHGADGV
     YHIVGNVRIG STDWADLREE YGEPQVYMKP VPVIPNVYIP RWFILGYEE