MCP5_SCHPO
ID MCP5_SCHPO Reviewed; 968 AA.
AC Q9Y7J9;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Meiotic coiled-coil protein 5;
DE AltName: Full=Meiotically up-regulated gene 21 protein;
DE AltName: Full=Nuclear migration protein num1;
GN Name=mcp5; Synonyms=mug21, num1; ORFNames=SPBC216.02;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16585273; DOI=10.1083/jcb.200512129;
RA Saito T.T., Okuzaki D., Nojima H.;
RT "Mcp5, a meiotic cell cortex protein, is required for nuclear movement
RT mediated by dynein and microtubules in fission yeast.";
RL J. Cell Biol. 173:27-33(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [4]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=16624923; DOI=10.1534/genetics.105.050062;
RA Yamashita A., Yamamoto M.;
RT "Fission yeast Num1p is a cortical factor anchoring dynein and is essential
RT for the horse-tail nuclear movement during meiotic prophase.";
RL Genetics 173:1187-1196(2006).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in chromosome segregation. Facilitates nuclear
CC oscillation during meiotic prophase I, the 'horsetail' phase, by acting
CC as a dynein anchor. Essential for anchoring dhc1 to the cortex.
CC Required for proper sliding of the microtubules along the cell cortex,
CC which leads to the proper U-turn movement of the nucleus.
CC {ECO:0000269|PubMed:16303567, ECO:0000269|PubMed:16585273,
CC ECO:0000269|PubMed:16624923}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:16585273, ECO:0000269|PubMed:16624923,
CC ECO:0000269|PubMed:16823372}. Note=Localizes to the cell cortex during
CC the 'horsetail' phase of mitosis.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB189987; BAD42849.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB40162.1; -; Genomic_DNA.
DR PIR; T39908; T39908.
DR RefSeq; NP_595354.1; NM_001021261.2.
DR AlphaFoldDB; Q9Y7J9; -.
DR SMR; Q9Y7J9; -.
DR BioGRID; 277235; 17.
DR STRING; 4896.SPBC216.02.1; -.
DR PaxDb; Q9Y7J9; -.
DR PRIDE; Q9Y7J9; -.
DR EnsemblFungi; SPBC216.02.1; SPBC216.02.1:pep; SPBC216.02.
DR GeneID; 2540712; -.
DR KEGG; spo:SPBC216.02; -.
DR PomBase; SPBC216.02; mcp5.
DR VEuPathDB; FungiDB:SPBC216.02; -.
DR eggNOG; ENOG502QWE7; Eukaryota.
DR HOGENOM; CLU_306117_0_0_1; -.
DR InParanoid; Q9Y7J9; -.
DR OMA; LRYAWIH; -.
DR PhylomeDB; Q9Y7J9; -.
DR PRO; PR:Q9Y7J9; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005938; C:cell cortex; IDA:PomBase.
DR GO; GO:0106186; C:cytoplasmic side of plasma membrane, cell tip; IDA:PomBase.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IDA:PomBase.
DR GO; GO:0031315; C:extrinsic component of mitochondrial outer membrane; IDA:PomBase.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0031520; C:plasma membrane of cell tip; IDA:PomBase.
DR GO; GO:1901612; F:cardiolipin binding; IDA:PomBase.
DR GO; GO:0106006; F:cytoskeletal protein-membrane anchor activity; IDA:PomBase.
DR GO; GO:0140443; F:mitochondrion-plasma membrane adaptor activity; IMP:PomBase.
DR GO; GO:0070300; F:phosphatidic acid binding; IDA:PomBase.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; EXP:PomBase.
DR GO; GO:0015631; F:tubulin binding; IBA:GO_Central.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0030989; P:dynein-driven meiotic oscillatory nuclear movement; IBA:GO_Central.
DR GO; GO:0051654; P:establishment of mitochondrion localization; EXP:PomBase.
DR GO; GO:0032118; P:horsetail-astral microtubule organization; IMP:PomBase.
DR GO; GO:0032065; P:maintenance of protein location in cell cortex; IEA:InterPro.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; IBA:GO_Central.
DR GO; GO:0051646; P:mitochondrion localization; IMP:PomBase.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR024774; PH_dom-Mcp5-type.
DR InterPro; IPR001849; PH_domain.
DR Pfam; PF12814; Mcp5_PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Chromosome partition; Coiled coil; Cytoplasm; Meiosis; Reference proteome.
FT CHAIN 1..968
FT /note="Meiotic coiled-coil protein 5"
FT /id="PRO_0000096291"
FT DOMAIN 847..958
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT COILED 68..91
FT /evidence="ECO:0000255"
FT COILED 152..240
FT /evidence="ECO:0000255"
SQ SEQUENCE 968 AA; 111374 MW; FD60114FA9372707 CRC64;
MEKKQDNEVE LYSIDLKNRN ESAKIKLAYA KALEESILCE TRKIEQEIKD QVVQKENKTY
FNNKFDNRYK LRDLEKNLAE EEVRITEMLT IQRQDIAIER RKNTNCFSES SQVDQYSVTE
TNLQYATNIG LNLLQQLRYL RLQLSEKEDL HKKLSVDNAH LIKQLDLLSS NMKTLMKEKT
KVQGQRDLLE QRLQGLMKKL TEVESLTVSL NDEKNKLTLE LTNLRICLHE LQLNAEKGET
IDESEDSKNT LVTEDENDDS VFLESSSDKY FDSSSFDAEQ EEKHDFPIDP FLSLESNVQT
SVSQSSAVLK SINLAKQELV SVNHLVADDT KTPSPNLSSE IIENTKADIK KSIRSLNKAV
SPENSIDIED ETDRDDEFIC FLLSYQQLQK ALKSNLGLFQ LSCLYPLSLN HLIQSFFQSL
TSQISIKDID SKNKQVIKLF KPTKSSDRQE IFELAQFEPR QKFPIDTNSS KCDMSASTEM
HLKDTEKFAN FKLKENILNH SSIYERDVSY LPALNKGGRE NYLNNRVSSY RLVKPILNSY
GNHVLNRIQH DILENHFCCL KNVKDFVCGA NTISMQWNLQ KNMEFISSVF SLIKNEVNDT
SANSVILRTD ALSNVATTRL KRKLTSAIID CSIPRRRLRH YSNPEIAEFK NFRLSTDISH
SNVRNSKKLC SNGSMNCMRQ GKAVFPRKIP YQEHDWEQWE KIAQSSMFKN RSIASRQSLE
TSDVTLDINM SSRHSNGTSS MLFKDFTKHN NWEASTYPSA PKNTRTYPTA EVEKTRHDSS
QSARQLKARS TATTISISLS TVSDVFTLPR NNLKSKTNTK KCRDNLNLSG LSSSTCNANS
VNKLMKHVMM GNEMMKYPRS ESFKMFKKSH WRYFWINPHC EMLCWSKTNP FIEKGRKNQV
KSVKIKGMTI IKAPDALKNA NLHNEVIVMQ TTDKPIVLKA KSKKIHNIWV QAIKQINHKA
IFDTKPGI
