MCP6_SCHPO
ID MCP6_SCHPO Reviewed; 327 AA.
AC Q10336;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Meiotic coiled-coil protein 6;
DE AltName: Full=Horsetail movement protein hrs1;
DE AltName: Full=Meiotically up-regulated gene 3 protein;
GN Name=mcp6; Synonyms=hrs1, mug3; ORFNames=SPBC582.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15654021; DOI=10.1242/jcs.01629;
RA Saito T.T., Tougan T., Okuzaki D., Kasama T., Nojima H.;
RT "Mcp6, a meiosis-specific coiled-coil protein of Schizosaccharomyces pombe,
RT localizes to the spindle pole body and is required for horsetail movement
RT and recombination.";
RL J. Cell Sci. 118:447-459(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP INTERACTION WITH MBO1.
RX PubMed=15120067; DOI=10.1016/j.cub.2004.03.042;
RA Sawin K.E., Lourenco P.C.C., Snaith H.A.;
RT "Microtubule nucleation at non-spindle pole body microtubule-organizing
RT centers requires fission yeast centrosomin-related protein mod20p.";
RL Curr. Biol. 14:763-775(2004).
RN [4]
RP FUNCTION, INTERACTION WITH ALP4 AND KMS1, AND SUBCELLULAR LOCATION.
RX PubMed=16111942; DOI=10.1016/j.cub.2005.07.058;
RA Tanaka K., Kohda T., Yamashita A., Nonaka N., Yamamoto M.;
RT "Hrs1p/Mcp6p on the meiotic SPB organizes astral microtubule arrays for
RT oscillatory nuclear movement.";
RL Curr. Biol. 15:1479-1486(2005).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Has a role in meiotic nuclear oscillation and recombination.
CC Required to remodel astral microtubules into the 'horsetail' astral
CC array maintaining the 'horsetail' nuclear movement. Promotes homologous
CC paring of chromosomes during this movement.
CC {ECO:0000269|PubMed:15654021, ECO:0000269|PubMed:16111942}.
CC -!- SUBUNIT: Interacts with alp4, kms1 and mbo1.
CC {ECO:0000269|PubMed:15120067, ECO:0000269|PubMed:16111942}.
CC -!- INTERACTION:
CC Q10336; Q9Y705: alp4; NbExp=2; IntAct=EBI-1562149, EBI-1562228;
CC Q10336; P87245: kms1; NbExp=2; IntAct=EBI-1562149, EBI-1542265;
CC Q10336; Q10336: mcp6; NbExp=2; IntAct=EBI-1562149, EBI-1562149;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, microtubule
CC organizing center, spindle pole body. Note=Localizes to the spindle
CC pole body until the onset of meiosis I.
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DR EMBL; AB189986; BAD42848.1; -; Genomic_DNA.
DR EMBL; CU329671; CAB46669.1; -; Genomic_DNA.
DR PIR; T37977; T37977.
DR RefSeq; NP_595174.1; NM_001021082.1.
DR AlphaFoldDB; Q10336; -.
DR SMR; Q10336; -.
DR BioGRID; 277612; 2.
DR IntAct; Q10336; 4.
DR STRING; 4896.SPBC582.06c.1; -.
DR PaxDb; Q10336; -.
DR EnsemblFungi; SPBC582.06c.1; SPBC582.06c.1:pep; SPBC582.06c.
DR GeneID; 2541097; -.
DR KEGG; spo:SPBC582.06c; -.
DR PomBase; SPBC582.06c; mcp6.
DR VEuPathDB; FungiDB:SPBC582.06c; -.
DR HOGENOM; CLU_850354_0_0_1; -.
DR OMA; FLKLQMN; -.
DR PhylomeDB; Q10336; -.
DR PRO; PR:Q10336; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0035974; C:meiotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0030989; P:dynein-driven meiotic oscillatory nuclear movement; IMP:PomBase.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:PomBase.
DR GO; GO:0032118; P:horsetail-astral microtubule organization; IMP:PomBase.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..327
FT /note="Meiotic coiled-coil protein 6"
FT /id="PRO_0000096292"
FT REGION 175..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 66..188
FT /evidence="ECO:0000255"
FT COILED 243..297
FT /evidence="ECO:0000255"
FT COMPBIAS 175..199
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 200..234
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 327 AA; 38104 MW; 095BF3C8AA9F125E CRC64;
MEYQEEASLA SAEDSTFIAS SPVQSVSEMK SIKQKSFQYF DDYEKNVSDK TIQFQKLQMT
AKEIIDAFER DSTQRTLQIE SLESKIGEQE RDLNNEKLAS ETLREKTQLL EKENGALKVE
NGYLYEKSRK LEEEMAHLKK KCNVYKSKFE ESSLRCKSLY SSNTKLKDSM ETMKRRMETE
TKEMNKIKPK NDSESDRFKR NSQSLSQQSP LLDVHSPDNS NHRTMLNINN SSPIKPKKIF
KPNEVKNRIS RLQKTFADLE NQHHSFQQIC QTLRKRLEND SSTTKQRLSK LEEIIRNRAP
PSYSFSLNCS HTYQPVSCVE PVNHDLS
