MCRC_ECOLI
ID MCRC_ECOLI Reviewed; 348 AA.
AC P15006; Q2M5X1;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 3.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Type IV methyl-directed restriction enzyme EcoKMcrBC {ECO:0000303|PubMed:12654995};
DE Short=EcoKMcrBC {ECO:0000303|PubMed:12654995};
DE AltName: Full=Protein McrC;
GN Name=mcrC; OrderedLocusNames=b4345, JW5789;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2203735; DOI=10.1128/jb.172.9.4888-4900.1990;
RA Dila D., Sutherland E., Moran L., Slatko B., Raleigh E.A.;
RT "Genetic and sequence organization of the mcrBC locus of Escherichia coli
RT K-12.";
RL J. Bacteriol. 172:4888-4900(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=2649480; DOI=10.1128/jb.171.4.1974-1981.1989;
RA Ross T.K., Achberger E.C., Braymer H.D.;
RT "Nucleotide sequence of the McrB region of Escherichia coli K-12 and
RT evidence for two independent translational initiation sites at the mcrB
RT locus.";
RL J. Bacteriol. 171:1974-1981(1989).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP PROTEIN SEQUENCE OF 1-10.
RC STRAIN=K12;
RX PubMed=2050643; DOI=10.1128/jb.173.12.3918-3920.1991;
RA Zheng L., Braymer H.D.;
RT "Overproduction and purification of McrC protein from Escherichia coli K-
RT 12.";
RL J. Bacteriol. 173:3918-3920(1991).
RN [7]
RP ERRATUM OF PUBMED:2050643.
RA Zheng L., Braymer H.D.;
RL J. Bacteriol. 173:5933-5933(1991).
RN [8]
RP NOMENCLATURE.
RX PubMed=12654995; DOI=10.1093/nar/gkg274;
RA Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT endonucleases and their genes.";
RL Nucleic Acids Res. 31:1805-1812(2003).
CC -!- FUNCTION: Modifies the specificity of McrB restriction by expanding the
CC range of modified sequences restricted. Does not bind to DNA.
CC -!- INTERACTION:
CC P15006; P15005: mcrB; NbExp=2; IntAct=EBI-25407271, EBI-552513;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA24146.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M58752; AAA24146.1; ALT_INIT; Genomic_DNA.
DR EMBL; M24927; AAA24144.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97242.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77301.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78335.1; -; Genomic_DNA.
DR PIR; JS0121; BVECMB.
DR RefSeq; NP_418765.1; NC_000913.3.
DR RefSeq; WP_000437621.1; NZ_LN832404.1.
DR PDB; 6HZ4; EM; 3.60 A; M/N=1-348.
DR PDB; 6HZ5; EM; 4.20 A; M/N=1-348.
DR PDB; 6HZ6; EM; 4.30 A; M/N=1-348.
DR PDB; 6HZ7; EM; 4.30 A; M/N=1-348.
DR PDB; 6HZ8; EM; 4.30 A; M/N=1-348.
DR PDB; 6HZ9; EM; 4.80 A; M/N=1-348.
DR PDB; 6UT6; EM; 3.28 A; G=1-348.
DR PDBsum; 6HZ4; -.
DR PDBsum; 6HZ5; -.
DR PDBsum; 6HZ6; -.
DR PDBsum; 6HZ7; -.
DR PDBsum; 6HZ8; -.
DR PDBsum; 6HZ9; -.
DR PDBsum; 6UT6; -.
DR AlphaFoldDB; P15006; -.
DR SMR; P15006; -.
DR BioGRID; 4262764; 181.
DR ComplexPortal; CPX-5285; McrBC 5-methylcytosine-specific restriction endonuclease complex.
DR IntAct; P15006; 1.
DR STRING; 511145.b4345; -.
DR REBASE; 13377; EcoW3110McrBCP.
DR REBASE; 2865; EcoKMcrBC.
DR REBASE; 441251; EcoBL21FMcrBCP.
DR PaxDb; P15006; -.
DR PRIDE; P15006; -.
DR EnsemblBacteria; AAC77301; AAC77301; b4345.
DR EnsemblBacteria; BAE78335; BAE78335; BAE78335.
DR GeneID; 948880; -.
DR KEGG; ecj:JW5789; -.
DR KEGG; eco:b4345; -.
DR PATRIC; fig|1411691.4.peg.2341; -.
DR EchoBASE; EB0570; -.
DR eggNOG; COG4268; Bacteria.
DR HOGENOM; CLU_065564_0_0_6; -.
DR InParanoid; P15006; -.
DR OMA; IPIRNLW; -.
DR BioCyc; EcoCyc:EG10575-MON; -.
DR BioCyc; MetaCyc:EG10575-MON; -.
DR PRO; PR:P15006; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1905348; C:endonuclease complex; IPI:ComplexPortal.
DR GO; GO:0032067; F:type IV site-specific deoxyribonuclease activity; IDA:EcoCyc.
DR GO; GO:0009307; P:DNA restriction-modification system; IC:ComplexPortal.
DR InterPro; IPR019292; McrC.
DR InterPro; IPR014407; McrC_bac.
DR PANTHER; PTHR38733; PTHR38733; 1.
DR Pfam; PF10117; McrBC; 1.
DR PIRSF; PIRSF003109; McrC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Restriction system.
FT CHAIN 1..348
FT /note="Type IV methyl-directed restriction enzyme
FT EcoKMcrBC"
FT /id="PRO_0000077381"
FT HELIX 8..18
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 29..32
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 38..53
FT /evidence="ECO:0007829|PDB:6UT6"
FT TURN 54..56
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 61..71
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 74..76
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 80..83
FT /evidence="ECO:0007829|PDB:6UT6"
FT TURN 84..88
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 92..101
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 104..117
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 118..122
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 124..135
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 147..150
FT /evidence="ECO:0007829|PDB:6UT6"
FT TURN 151..153
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 154..157
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 162..174
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 175..177
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 185..187
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 194..204
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 206..210
FT /evidence="ECO:0007829|PDB:6UT6"
FT TURN 211..213
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 244..249
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 252..258
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 263..266
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 279..288
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 297..302
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 306..308
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 312..315
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 317..319
FT /evidence="ECO:0007829|PDB:6UT6"
FT STRAND 321..324
FT /evidence="ECO:0007829|PDB:6UT6"
FT HELIX 332..344
FT /evidence="ECO:0007829|PDB:6UT6"
SQ SEQUENCE 348 AA; 40590 MW; 9B65FAD20E5C6E64 CRC64;
MEQPVIPVRN IYYMLTYAWG YLQEIKQANL EAIPGNNLLD ILGYVLNKGV LQLSRRGLEL
DYNPNTEIIP GIKGRIEFAK TIRGFHLNHG KTVSTFDMLN EDTLANRIIK STLAILIKHE
KLNSTIRDEA RSLYRKLPGI STLHLTPQHF SYLNGGKNTR YYKFVISVCK FIVNNSIPGQ
NKGHYRFYDF ERNEKEMSLL YQKFLYEFCR RELTSANTTR SYLKWDASSI SDQSLNLLPR
METDITIRSS EKILIVDAKY YKSIFSRRMG TEKFHSQNLY QLMNYLWSLK PENGENIGGL
LIYPHVDTAV KHRYKINGFD IGLCTVNLGQ EWPCIHQELL DIFDEYLK