MCRI1_RAT
ID MCRI1_RAT Reviewed; 97 AA.
AC B0BN72;
DT 18-MAY-2010, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Mapk-regulated corepressor-interacting protein 1;
DE AltName: Full=Protein FAM195B;
GN Name=Mcrip1; Synonyms=Fam195b;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Heart;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: The phosphorylation status of MCRIP1 functions as a molecular
CC switch to regulate epithelial-mesenchymal transition. Unphosphorylated
CC MCRIP1 binds to and inhibits the transcriptional corepressor CTBP(s).
CC When phosphorylated by MAPK/ERK, MCRIP1 releases CTBP(s) resulting in
CC transcriptional silencing of the E-cadherin gene and induction of
CC epithelial-mesenchymal transition. {ECO:0000250|UniProtKB:C9JLW8}.
CC -!- SUBUNIT: Interacts (unphosphorylated form, via the PXDLS motif) with
CC CTBP1, competitively inhibiting CTBP-ZEB1 interaction. Interacts with
CC CTBP2. Interacts with MCRIP2. Interacts with DDX6.
CC {ECO:0000250|UniProtKB:C9JLW8}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C9JLW8}.
CC Cytoplasm, Stress granule {ECO:0000250|UniProtKB:C9JLW8}.
CC -!- PTM: Phosphorylation by MAPK3/1 (ERK1/2) regulates MCRIP1 binding to
CC CTBP(s). {ECO:0000250|UniProtKB:C9JLW8}.
CC -!- SIMILARITY: Belongs to the MCRIP family. {ECO:0000305}.
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DR EMBL; CH473948; EDM06851.1; -; Genomic_DNA.
DR EMBL; BC158709; AAI58710.1; -; mRNA.
DR RefSeq; NP_001101781.1; NM_001108311.1.
DR RefSeq; XP_006247958.1; XM_006247896.3.
DR RefSeq; XP_017452903.1; XM_017597414.1.
DR AlphaFoldDB; B0BN72; -.
DR SMR; B0BN72; -.
DR STRING; 10116.ENSRNOP00000051843; -.
DR iPTMnet; B0BN72; -.
DR PhosphoSitePlus; B0BN72; -.
DR jPOST; B0BN72; -.
DR PaxDb; B0BN72; -.
DR PeptideAtlas; B0BN72; -.
DR PRIDE; B0BN72; -.
DR GeneID; 360677; -.
DR KEGG; rno:360677; -.
DR CTD; 348262; -.
DR RGD; 1311925; Mcrip1.
DR VEuPathDB; HostDB:ENSRNOG00000036691; -.
DR eggNOG; ENOG502S25D; Eukaryota.
DR HOGENOM; CLU_161057_0_0_1; -.
DR InParanoid; B0BN72; -.
DR OMA; QNHERND; -.
DR OrthoDB; 1461250at2759; -.
DR PhylomeDB; B0BN72; -.
DR TreeFam; TF326620; -.
DR PRO; PR:B0BN72; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Proteomes; UP000234681; Chromosome 10.
DR Bgee; ENSRNOG00000036691; Expressed in heart and 18 other tissues.
DR Genevisible; B0BN72; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0010717; P:regulation of epithelial to mesenchymal transition; ISS:UniProtKB.
DR InterPro; IPR029428; MCRIP.
DR Pfam; PF14799; FAM195; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..97
FT /note="Mapk-regulated corepressor-interacting protein 1"
FT /id="PRO_0000393956"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 80..84
FT /note="PXDLS motif"
FT /evidence="ECO:0000305"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 30
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:C9JLW8"
FT MOD_RES 41
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q3UGS4"
FT MOD_RES 79
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:C9JLW8"
SQ SEQUENCE 97 AA; 11101 MW; 50792192F1C63720 CRC64;
MTSSPVSRVV YNGKRNSSPR SPTNSSEIFT PAHEENVRFI YEAWQGVERD LRSQLSSGER
CLVEEYVEKV PNPSLKTFKP IDLSDLKRRN TQDAKKS
