MCRI2_HUMAN
ID MCRI2_HUMAN Reviewed; 160 AA.
AC Q9BUT9; Q969E9; Q96KV8;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=MAPK regulated corepressor interacting protein 2;
DE AltName: Full=Protein FAM195A;
GN Name=MCRIP2; Synonyms=C16orf14, FAM195A;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Pancreas, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-61 AND SER-82, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=20068231; DOI=10.1126/scisignal.2000475;
RA Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L.,
RA Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.;
RT "Quantitative phosphoproteomics reveals widespread full phosphorylation
RT site occupancy during mitosis.";
RL Sci. Signal. 3:RA3-RA3(2010).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
RN [9]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-35 AND ARG-65, AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Colon carcinoma;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
RN [10]
RP INTERACTION WITH DDX6 AND MCRIP1, AND SUBCELLULAR LOCATION.
RX PubMed=26184334; DOI=10.3390/biom5031441;
RA Bish R., Cuevas-Polo N., Cheng Z., Hambardzumyan D., Munschauer M.,
RA Landthaler M., Vogel C.;
RT "Comprehensive protein interactome analysis of a key RNA helicase:
RT detection of novel stress granule proteins.";
RL Biomolecules 5:1441-1466(2015).
CC -!- SUBUNIT: Interacts with DDX6. Interacts with MCRIP1.
CC {ECO:0000269|PubMed:26184334}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, Stress granule
CC {ECO:0000269|PubMed:26184334}. Nucleus {ECO:0000269|PubMed:26184334}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9BUT9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BUT9-2; Sequence=VSP_022712;
CC -!- MISCELLANEOUS: [Isoform 2]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MCRIP family. {ECO:0000305}.
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DR EMBL; AL022341; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC001912; AAH01912.2; -; mRNA.
DR EMBL; BC007346; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC111391; AAI11392.1; -; mRNA.
DR CCDS; CCDS10415.1; -. [Q9BUT9-1]
DR RefSeq; NP_001318158.1; NM_001331229.1.
DR RefSeq; NP_001318159.1; NM_001331230.1.
DR RefSeq; NP_612427.2; NM_138418.3. [Q9BUT9-1]
DR AlphaFoldDB; Q9BUT9; -.
DR BioGRID; 124055; 52.
DR IntAct; Q9BUT9; 7.
DR STRING; 9606.ENSP00000305138; -.
DR GlyGen; Q9BUT9; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9BUT9; -.
DR PhosphoSitePlus; Q9BUT9; -.
DR BioMuta; MCRIP2; -.
DR DMDM; 74752367; -.
DR EPD; Q9BUT9; -.
DR jPOST; Q9BUT9; -.
DR MassIVE; Q9BUT9; -.
DR MaxQB; Q9BUT9; -.
DR PaxDb; Q9BUT9; -.
DR PeptideAtlas; Q9BUT9; -.
DR PRIDE; Q9BUT9; -.
DR ProteomicsDB; 79128; -. [Q9BUT9-1]
DR ProteomicsDB; 79129; -. [Q9BUT9-2]
DR Antibodypedia; 42373; 23 antibodies from 14 providers.
DR DNASU; 84331; -.
DR Ensembl; ENST00000307650.9; ENSP00000305138.4; ENSG00000172366.20. [Q9BUT9-1]
DR Ensembl; ENST00000474840.5; ENSP00000433876.1; ENSG00000172366.20. [Q9BUT9-2]
DR GeneID; 84331; -.
DR KEGG; hsa:84331; -.
DR MANE-Select; ENST00000307650.9; ENSP00000305138.4; NM_138418.4; NP_612427.2.
DR UCSC; uc002cic.2; human. [Q9BUT9-1]
DR CTD; 84331; -.
DR GeneCards; MCRIP2; -.
DR HGNC; HGNC:14142; MCRIP2.
DR HPA; ENSG00000172366; Tissue enhanced (liver).
DR neXtProt; NX_Q9BUT9; -.
DR OpenTargets; ENSG00000172366; -.
DR PharmGKB; PA25527; -.
DR VEuPathDB; HostDB:ENSG00000172366; -.
DR eggNOG; ENOG502RZDA; Eukaryota.
DR GeneTree; ENSGT00940000160332; -.
DR HOGENOM; CLU_121013_0_0_1; -.
DR InParanoid; Q9BUT9; -.
DR OMA; YESWNQV; -.
DR OrthoDB; 1461250at2759; -.
DR PhylomeDB; Q9BUT9; -.
DR TreeFam; TF326620; -.
DR PathwayCommons; Q9BUT9; -.
DR SignaLink; Q9BUT9; -.
DR BioGRID-ORCS; 84331; 9 hits in 1080 CRISPR screens.
DR ChiTaRS; MCRIP2; human.
DR GenomeRNAi; 84331; -.
DR Pharos; Q9BUT9; Tdark.
DR PRO; PR:Q9BUT9; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9BUT9; protein.
DR Bgee; ENSG00000172366; Expressed in mucosa of transverse colon and 175 other tissues.
DR ExpressionAtlas; Q9BUT9; baseline and differential.
DR Genevisible; Q9BUT9; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0010494; C:cytoplasmic stress granule; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR InterPro; IPR029428; MCRIP.
DR Pfam; PF14799; FAM195; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Methylation; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..160
FT /note="MAPK regulated corepressor interacting protein 2"
FT /id="PRO_0000274328"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..61
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT MOD_RES 35
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648"
FT MOD_RES 65
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 82
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18669648,
FT ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"
FT VAR_SEQ 62..160
FT /note="PGPRLVFNRVNGRRAPSTSPSFEGTQETYTVAHEENVRFVSEAWQQVQQQLD
FT GGPAGEGGPRPVQYVERTPNPRLQNFVPIDLDEWWAQQFLARITSCS -> LAAGATAA
FT GWWPSR (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_022712"
SQ SEQUENCE 160 AA; 17828 MW; 153139F535B0AF65 CRC64;
MYTITKGPSK LVAQRRTGPT QQQVEGRLGE LLKCRQPAPP TSQPPRAQPF AQPPGPWPLS
SPGPRLVFNR VNGRRAPSTS PSFEGTQETY TVAHEENVRF VSEAWQQVQQ QLDGGPAGEG
GPRPVQYVER TPNPRLQNFV PIDLDEWWAQ QFLARITSCS
