MCRI2_MOUSE
ID MCRI2_MOUSE Reviewed; 160 AA.
AC Q9CQB2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=MAPK regulated corepressor interacting protein 2;
DE AltName: Full=Protein FAM195A;
GN Name=Mcrip2; Synonyms=Fam195a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Urinary bladder;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-82, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP METHYLATION [LARGE SCALE ANALYSIS] AT ARG-35 AND ARG-65, AND IDENTIFICATION
RP BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- SUBUNIT: Interacts with DDX6. Interacts with MCRIP1.
CC {ECO:0000250|UniProtKB:Q9BUT9}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, Stress granule
CC {ECO:0000250|UniProtKB:Q9BUT9}. Nucleus {ECO:0000250|UniProtKB:Q9BUT9}.
CC Note=Upon cellular stress, relocalizes to stress granules.
CC {ECO:0000250|UniProtKB:Q9BUT9}.
CC -!- SIMILARITY: Belongs to the MCRIP family. {ECO:0000305}.
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DR EMBL; AK003564; BAB22860.1; -; mRNA.
DR EMBL; AK020614; BAB32148.1; -; mRNA.
DR EMBL; BC024332; AAH24332.1; -; mRNA.
DR CCDS; CCDS28535.1; -.
DR RefSeq; NP_080909.1; NM_026633.3.
DR AlphaFoldDB; Q9CQB2; -.
DR IntAct; Q9CQB2; 1.
DR MINT; Q9CQB2; -.
DR STRING; 10090.ENSMUSP00000026828; -.
DR iPTMnet; Q9CQB2; -.
DR PhosphoSitePlus; Q9CQB2; -.
DR EPD; Q9CQB2; -.
DR jPOST; Q9CQB2; -.
DR MaxQB; Q9CQB2; -.
DR PaxDb; Q9CQB2; -.
DR PeptideAtlas; Q9CQB2; -.
DR PRIDE; Q9CQB2; -.
DR ProteomicsDB; 292201; -.
DR Antibodypedia; 42373; 23 antibodies from 14 providers.
DR DNASU; 68241; -.
DR Ensembl; ENSMUST00000026828; ENSMUSP00000026828; ENSMUSG00000025732.
DR GeneID; 68241; -.
DR KEGG; mmu:68241; -.
DR UCSC; uc008bck.1; mouse.
DR CTD; 84331; -.
DR MGI; MGI:1915491; Mcrip2.
DR VEuPathDB; HostDB:ENSMUSG00000025732; -.
DR eggNOG; ENOG502RZDA; Eukaryota.
DR GeneTree; ENSGT00940000160332; -.
DR HOGENOM; CLU_121013_0_0_1; -.
DR InParanoid; Q9CQB2; -.
DR OMA; YESWNQV; -.
DR OrthoDB; 1461250at2759; -.
DR PhylomeDB; Q9CQB2; -.
DR TreeFam; TF326620; -.
DR BioGRID-ORCS; 68241; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Fam195a; mouse.
DR PRO; PR:Q9CQB2; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9CQB2; protein.
DR Bgee; ENSMUSG00000025732; Expressed in interventricular septum and 226 other tissues.
DR ExpressionAtlas; Q9CQB2; baseline and differential.
DR Genevisible; Q9CQB2; MM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0010494; C:cytoplasmic stress granule; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR InterPro; IPR029428; MCRIP.
DR Pfam; PF14799; FAM195; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Methylation; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..160
FT /note="MAPK regulated corepressor interacting protein 2"
FT /id="PRO_0000274329"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 43..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUT9"
FT MOD_RES 35
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BUT9"
FT MOD_RES 65
FT /note="Omega-N-methylarginine"
FT /evidence="ECO:0007744|PubMed:24129315"
FT MOD_RES 82
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 160 AA; 17871 MW; A5AE2689E9C84DEF CRC64;
MYTITKGPSK LVAQRRTGPT QQQVESRLGE LLKCRQPVPP TALPAHLQPS AQTQGPWPLA
SSGPRLVFNR VNGRRPLTTS PSLEGTQETY TVAHEENVRF VSEAWQQVER QLDGGPADES
GPRPVQYVES TPDPRLQNFV PIDLDEWWAQ QFLAKITNCS
