MCSP_MOUSE
ID MCSP_MOUSE Reviewed; 143 AA.
AC P15265; O70613; Q6P8N3;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2003, sequence version 4.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Sperm mitochondrial-associated cysteine-rich protein;
GN Name=Smcp; Synonyms=Mcs, Mcsp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=CD-1; TISSUE=Testis;
RX PubMed=2303168; DOI=10.1016/0012-1606(90)90263-i;
RA Kleene K.C., Smith J., Bozorgzadeh A., Harris M., Hahn L., Karimpour I.,
RA Gerstel J.;
RT "Sequence and developmental expression of the mRNA encoding the seleno-
RT protein of the sperm mitochondrial capsule in the mouse.";
RL Dev. Biol. 137:395-402(1990).
RN [2]
RP SEQUENCE REVISION TO N-TERMINUS.
RA Kleene K.C.;
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ; TISSUE=Liver;
RX PubMed=1418626; DOI=10.1089/dna.1992.11.693;
RA Karimpour I., Cutler M., Shih D., Smith J., Kleene K.C.;
RT "Sequence of the gene encoding the mitochondrial capsule selenoprotein of
RT mouse sperm: identification of three in-phase TGA selenocysteine codons.";
RL DNA Cell Biol. 11:693-699(1992).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=8916043;
RX DOI=10.1002/(sici)1098-2795(199611)45:3<320::aid-mrd9>3.0.co;2-u;
RA Cataldo L., Baig K., Oko R., Mastrangelo M.A., Kleene K.C.;
RT "Developmental expression, intracellular localization, and selenium content
RT of the cysteine-rich protein associated with the mitochondrial capsules of
RT mouse sperm.";
RL Mol. Reprod. Dev. 45:320-331(1996).
RN [7]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=9409512; DOI=10.1292/jvms.59.983;
RA Nam S.-Y., Maeda S., Ogawa K., Kurohmaru M., Hayashi Y.;
RT "Expression pattern of the mitochondrial capsule selenoprotein mRNA in the
RT mouse testis after puberty; in situ hybridization study.";
RL J. Vet. Med. Sci. 59:983-988(1997).
RN [8]
RP FUNCTION.
RX PubMed=11940662; DOI=10.1128/mcb.22.9.3046-3052.2002;
RA Nayernia K., Adham I.M., Burkhardt-Goettges E., Neesen J., Rieche M.,
RA Wolf S., Sancken U., Kleene K.C., Engel W.;
RT "Asthenozoospermia in mice with targeted deletion of the sperm
RT mitochondrion-associated cysteine-rich protein (Smcp) gene.";
RL Mol. Cell. Biol. 22:3046-3052(2002).
CC -!- FUNCTION: Involved in sperm motility. Its absence is associated with
CC genetic background dependent male infertility. Infertility may be due
CC to reduced sperm motility in the female reproductive tract and
CC inability to penetrate the oocyte zona pellucida.
CC {ECO:0000269|PubMed:11940662}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:8916043}.
CC Mitochondrion membrane {ECO:0000305}; Peripheral membrane protein
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}. Note=Becomes associated
CC with the spermatid mitochondrion capsule at step 16 of spermatogenesis.
CC -!- TISSUE SPECIFICITY: Testis. Is selectively expressed in the spermatids
CC of seminiferous tubules. {ECO:0000269|PubMed:2303168,
CC ECO:0000269|PubMed:8916043, ECO:0000269|PubMed:9409512}.
CC -!- DEVELOPMENTAL STAGE: First detected in step 11 spermatids. Levels
CC increase in subsequent steps to reach a maximum in late step 15 and
CC early step 16. Levels decrease in late step 16.
CC {ECO:0000269|PubMed:2303168, ECO:0000269|PubMed:8916043,
CC ECO:0000269|PubMed:9409512}.
CC -!- CAUTION: Was originally (PubMed:2303168 and PubMed:1418626) thought to
CC be a selenoprotein and was known as sperm mitochondrial capsule
CC selenoprotein. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB08438.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAC08283.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH55104.2; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAH61167.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAE43359.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M29603; AAA53045.3; -; mRNA.
DR EMBL; M88462; AAB08438.2; ALT_INIT; Genomic_DNA.
DR EMBL; S49657; AAC08282.2; -; Genomic_DNA.
DR EMBL; S49654; AAC08282.2; JOINED; Genomic_DNA.
DR EMBL; S49659; AAC08283.1; ALT_INIT; mRNA.
DR EMBL; AK077172; BAE43359.1; ALT_INIT; mRNA.
DR EMBL; BC055104; AAH55104.2; ALT_INIT; mRNA.
DR EMBL; BC061167; AAH61167.1; ALT_INIT; mRNA.
DR CCDS; CCDS89661.1; -.
DR PIR; T10081; T10081.
DR RefSeq; NP_032600.2; NM_008574.3.
DR AlphaFoldDB; P15265; -.
DR STRING; 10090.ENSMUSP00000096487; -.
DR PhosphoSitePlus; P15265; -.
DR PaxDb; P15265; -.
DR PeptideAtlas; P15265; -.
DR PRIDE; P15265; -.
DR ProteomicsDB; 252752; -.
DR DNASU; 17235; -.
DR Ensembl; ENSMUST00000098888; ENSMUSP00000096487; ENSMUSG00000074435.
DR Ensembl; ENSMUST00000194965; ENSMUSP00000142023; ENSMUSG00000074435.
DR GeneID; 17235; -.
DR KEGG; mmu:17235; -.
DR UCSC; uc008qeg.1; mouse.
DR CTD; 4184; -.
DR MGI; MGI:96945; Smcp.
DR VEuPathDB; HostDB:ENSMUSG00000074435; -.
DR eggNOG; ENOG502TF5H; Eukaryota.
DR GeneTree; ENSGT00730000114093; -.
DR HOGENOM; CLU_1805544_0_0_1; -.
DR InParanoid; P15265; -.
DR OMA; QPKCPQC; -.
DR OrthoDB; 1807370at2759; -.
DR BioGRID-ORCS; 17235; 2 hits in 51 CRISPR screens.
DR ChiTaRS; Smcp; mouse.
DR PRO; PR:P15265; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; P15265; protein.
DR Bgee; ENSMUSG00000074435; Expressed in seminiferous tubule of testis and 30 other tissues.
DR Genevisible; P15265; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005741; C:mitochondrial outer membrane; TAS:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IGI:MGI.
DR GO; GO:0030317; P:flagellated sperm motility; IDA:UniProtKB.
DR GO; GO:0007341; P:penetration of zona pellucida; IMP:MGI.
PE 2: Evidence at transcript level;
KW Cytoplasm; Fertilization; Membrane; Mitochondrion; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..143
FT /note="Sperm mitochondrial-associated cysteine-rich
FT protein"
FT /id="PRO_0000096308"
FT REGION 101..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64298"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64298"
FT MOD_RES 110
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64298"
FT MOD_RES 128
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q64298"
SQ SEQUENCE 143 AA; 14945 MW; 3BB16160002EC46A CRC64;
MSDPSKTNQC PPPCCPPKPC CPPKPCCPQK PPCCPKSPCC PPKSPCCPPK PCPCPPPCPC
PCPATCPCPL KPPCCPQKCS CCPKKCTCCP QPPPCCAQPT CCSSENKTES DSDTSGQTLE
KGSQSPQSPP GAQGNWNQKK SNK
