MCSP_RAT
ID MCSP_RAT Reviewed; 145 AA.
AC Q64298;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Sperm mitochondrial-associated cysteine-rich protein;
GN Name=Smcp; Synonyms=Mcs, Mcsp;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Testis;
RX PubMed=8634143; DOI=10.1089/dna.1996.15.159;
RA Adham I.M., Tessmann D., Soliman K.A., Murphy D., Kremling H., Szpirer C.,
RA Engel W.;
RT "Cloning, expression, and chromosomal localization of the rat mitochondrial
RT capsule selenoprotein gene (MCS): the reading frame does not contain
RT potential UGA selenocysteine codons.";
RL DNA Cell Biol. 15:159-166(1996).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=8916043;
RX DOI=10.1002/(sici)1098-2795(199611)45:3<320::aid-mrd9>3.0.co;2-u;
RA Cataldo L., Baig K., Oko R., Mastrangelo M.A., Kleene K.C.;
RT "Developmental expression, intracellular localization, and selenium content
RT of the cysteine-rich protein associated with the mitochondrial capsules of
RT mouse sperm.";
RL Mol. Reprod. Dev. 45:320-331(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND TISSUE SPECIFICITY.
RX PubMed=19423663; DOI=10.1530/rep-09-0052;
RA Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.;
RT "Identification of novel immunodominant epididymal sperm proteins using
RT combinatorial approach.";
RL Reproduction 138:81-93(2009).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-38; SER-45; SER-113 AND
RP SER-131, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Involved in sperm motility. Its absence is associated with
CC genetic background dependent male infertility. Infertility may be due
CC to reduced sperm motility in the female reproductive tract and
CC inability to penetrate the oocyte zona pellucida (By similarity).
CC {ECO:0000250|UniProtKB:P15265}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}; Cytoplasmic side
CC {ECO:0000305}. Note=Becomes associated with the spermatid mitochondrion
CC capsule at step 16 of spermatogenesis. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Testis. Selectively expressed in the spermatids of
CC seminiferous tubules and in flagella of epididymal sperm.
CC {ECO:0000269|PubMed:19423663, ECO:0000269|PubMed:8634143}.
CC -!- DEVELOPMENTAL STAGE: Expressed in postmeiotic cells.
CC {ECO:0000269|PubMed:8634143}.
CC -!- CAUTION: Was originally thought to be a selenoprotein and was known as
CC sperm mitochondrial capsule selenoprotein.
CC {ECO:0000305|PubMed:8634143}.
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DR EMBL; X87883; CAA61138.1; -; mRNA.
DR EMBL; U48702; AAB01896.1; -; mRNA.
DR EMBL; BC078795; AAH78795.1; -; mRNA.
DR RefSeq; NP_113724.1; NM_031536.2.
DR AlphaFoldDB; Q64298; -.
DR STRING; 10116.ENSRNOP00000012371; -.
DR iPTMnet; Q64298; -.
DR PhosphoSitePlus; Q64298; -.
DR PaxDb; Q64298; -.
DR Ensembl; ENSRNOT00000012371; ENSRNOP00000012371; ENSRNOG00000009326.
DR GeneID; 24899; -.
DR KEGG; rno:24899; -.
DR UCSC; RGD:3069; rat.
DR CTD; 4184; -.
DR RGD; 3069; Smcp.
DR eggNOG; ENOG502TF5H; Eukaryota.
DR GeneTree; ENSGT00730000114093; -.
DR HOGENOM; CLU_1805544_0_0_1; -.
DR InParanoid; Q64298; -.
DR OMA; QPKCPQC; -.
DR OrthoDB; 309992at32523; -.
DR PRO; PR:Q64298; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000009326; Expressed in testis and 9 other tissues.
DR Genevisible; Q64298; RN.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; ISO:RGD.
DR GO; GO:0030317; P:flagellated sperm motility; ISS:UniProtKB.
DR GO; GO:0007341; P:penetration of zona pellucida; ISO:RGD.
PE 1: Evidence at protein level;
KW Cytoplasm; Fertilization; Membrane; Mitochondrion; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..145
FT /note="Sperm mitochondrial-associated cysteine-rich
FT protein"
FT /id="PRO_0000096309"
FT REGION 105..145
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 38
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 45
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 113
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 131
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 145 AA; 15148 MW; 5BB70D1ACA86814D CRC64;
MSDSSKTNQC CPTPCCPPKP CCPPKPCCLQ KSPCCPKSPC CPPKSPCCTP KVCPCPTPCP
CPATCPAACA CPCPMKPCCP TKCTCCPKKC TCCPQPTCCV QPTCCSSENK TESDSDGSGQ
TQDRGAQTQQ SPQGGQGNWN QKKTK
