MCTP2_MOUSE
ID MCTP2_MOUSE Reviewed; 878 AA.
AC Q5RJH2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Multiple C2 and transmembrane domain-containing protein 2;
GN Name=Mctp2; Synonyms=Gm489;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP DEVELOPMENTAL STAGE.
RX PubMed=23773997; DOI=10.1093/hmg/ddt283;
RA Lalani S.R., Ware S.M., Wang X., Zapata G., Tian Q., Franco L.M., Jiang Z.,
RA Bucasas K., Scott D.A., Campeau P.M., Hanchard N., Umana L., Cast A.,
RA Patel A., Cheung S.W., McBride K.L., Bray M., Craig Chinault A.,
RA Boggs B.A., Huang M., Baker M.R., Hamilton S., Towbin J., Jefferies J.L.,
RA Fernbach S.D., Potocki L., Belmont J.W.;
RT "MCTP2 is a dosage-sensitive gene required for cardiac outflow tract
RT development.";
RL Hum. Mol. Genet. 22:4339-4348(2013).
CC -!- FUNCTION: Might play a role in the development of cardiac outflow
CC tract. {ECO:0000250|UniProtKB:Q6DN12}.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00041};
CC Note=Binds Ca(2+) via the C2 domains in absence of phospholipids.
CC {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels in the developing heart,
CC with high levels at 9.5 dpc that progressively decrease until 11.5 dpc.
CC {ECO:0000269|PubMed:23773997}.
CC -!- SIMILARITY: Belongs to the MCTP family. {ECO:0000305}.
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DR EMBL; BC086658; AAH86658.1; -; mRNA.
DR CCDS; CCDS21361.1; -.
DR RefSeq; NP_001019874.1; NM_001024703.1.
DR RefSeq; XP_006540962.1; XM_006540899.2.
DR AlphaFoldDB; Q5RJH2; -.
DR SMR; Q5RJH2; -.
DR STRING; 10090.ENSMUSP00000078302; -.
DR iPTMnet; Q5RJH2; -.
DR PhosphoSitePlus; Q5RJH2; -.
DR EPD; Q5RJH2; -.
DR jPOST; Q5RJH2; -.
DR PaxDb; Q5RJH2; -.
DR PRIDE; Q5RJH2; -.
DR ProteomicsDB; 293437; -.
DR Antibodypedia; 29077; 166 antibodies from 23 providers.
DR DNASU; 244049; -.
DR Ensembl; ENSMUST00000079323; ENSMUSP00000078302; ENSMUSG00000032776.
DR GeneID; 244049; -.
DR KEGG; mmu:244049; -.
DR UCSC; uc009hnp.1; mouse.
DR CTD; 55784; -.
DR MGI; MGI:2685335; Mctp2.
DR VEuPathDB; HostDB:ENSMUSG00000032776; -.
DR eggNOG; KOG1030; Eukaryota.
DR GeneTree; ENSGT00940000156291; -.
DR HOGENOM; CLU_011170_0_2_1; -.
DR InParanoid; Q5RJH2; -.
DR OMA; HFEEQSA; -.
DR OrthoDB; 234298at2759; -.
DR PhylomeDB; Q5RJH2; -.
DR TreeFam; TF323373; -.
DR BioGRID-ORCS; 244049; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Mctp2; mouse.
DR PRO; PR:Q5RJH2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q5RJH2; protein.
DR Bgee; ENSMUSG00000032776; Expressed in secondary oocyte and 113 other tissues.
DR ExpressionAtlas; Q5RJH2; baseline and differential.
DR Genevisible; Q5RJH2; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; ISS:HGNC-UCL.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0030672; C:synaptic vesicle membrane; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; ISS:HGNC-UCL.
DR GO; GO:0046928; P:regulation of neurotransmitter secretion; IBA:GO_Central.
DR Gene3D; 2.60.40.150; -; 3.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR013583; PRibTrfase_C.
DR Pfam; PF00168; C2; 3.
DR Pfam; PF08372; PRT_C; 1.
DR SMART; SM00239; C2; 3.
DR SUPFAM; SSF49562; SSF49562; 3.
DR PROSITE; PS50004; C2; 3.
PE 2: Evidence at transcript level;
KW Calcium; Developmental protein; Membrane; Metal-binding;
KW Reference proteome; Repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..878
FT /note="Multiple C2 and transmembrane domain-containing
FT protein 2"
FT /id="PRO_0000294473"
FT TRANSMEM 694..714
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 794..814
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 177..292
FT /note="C2 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 334..452
FT /note="C2 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 486..607
FT /note="C2 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT REGION 20..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 143..178
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 728..752
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..161
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 210
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 210
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 216
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 263
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 263
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 265
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 265
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 270
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 525
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 525
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 531
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 577
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 577
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 579
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 579
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT BINDING 585
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
SQ SEQUENCE 878 AA; 100178 MW; FAAC2F0497F4A36F CRC64;
MDLDKPSVWG SLKQRTRPLL INLSKKKAKK SPSKPLDLRV QHHLDRRLSL SVPDLLEAEA
LAPEGRPYSG PQSSYISVPN SLSTAGIVPK SSSSSLKQSE EELDWSQEEA SHVHGVDTDS
EEIYASPAEE WQAFSQSALD LHKPSLGRDA PEEHDKTHGN DDLNASMTSQ HFEEESTLGE
ASDCVSHLPS PFAYLLTIHL KEGRNLVVRD RCGTSDPYVK FKLNGKTLYK SKVIYKNLNP
IWDEIVVLPI QSLDQKLRVK VYDRDLTKSD FMGSAFVVLR DLELNRTTEH ILKLEDPNSL
EDDMGVIVLN LNLVVKQGDF KRHRWSNRKR LSASKSSLIR NLRLSESLRK NQLWNGIISI
TLLEGKNVSG GNMTEMFVQL KLGEQRYKSK TLCKSANPQW QEQFDFHYFS DRMGILDIEV
WGKDSKKHEE RLGTCKVDIS ALPLKQDNCL ELPLESCQGA LLMLITLTPC TGVSISDLCV
CPFEDPSERQ QISQRYAFQN SLKDVKDVGI LQVKVLKASD LLAADFSGKS DPFCLLELGN
DRLQTHTIYK NLNPEWNKVF TFPIKDIHDV LEVTVFDEDG DKAPDFLGKV AIPLLSIRDG
QPNCYVLKNK DLEQAFKGLI YLELDLIYNP VKASIRTFTP REKRFVEDSR KLSKKILSRD
VDRVKRLTLA IWNTVQFFKS CFQWESTLRS TIAFVVFLVT VWNFELYMIP LALLLLFLYN
FLRPMKGKAS STQDSQESTD VEEEGKEEEK ESEKKGIIER IYMVQDIVST VQNILEEVAS
FGERIKNVFN WTVPFLSLLA CLILAITTVI LYFIPLRYII LLWGINKFTK KLRNPYSIDN
NELLDFLSRV PSDIQKVQYA ELKLCGSHSP LRKKRSTV
