MCTS1_BOVIN
ID MCTS1_BOVIN Reviewed; 181 AA.
AC Q2KIE4;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Malignant T-cell-amplified sequence 1;
DE Short=MCT-1;
GN Name=MCTS1;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Testis;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Anti-oncogene that plays a role in cell cycle regulation;
CC decreases cell doubling time and anchorage-dependent growth; shortens
CC the duration of G1 transit time and G1/S transition. When
CC constitutively expressed, increases CDK4 and CDK6 kinases activity and
CC CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex
CC formation. Involved in translation initiation; promotes recruitment of
CC aminoacetyled initiator tRNA to P site of 40S ribosomes. Can promote
CC release of deacylated tRNA and mRNA from recycled 40S subunits
CC following ABCE1-mediated dissociation of post-termination ribosomal
CC complexes into subunits. Plays a role as translation enhancer; recruits
CC the density-regulated protein/DENR and binds to the cap complex of the
CC 5'-terminus of mRNAs, subsequently altering the mRNA translation
CC profile; up-regulates protein levels of BCL2L2, TFDP1, MRE11, CCND1 and
CC E2F1, while mRNA levels remains constant. Hyperactivates DNA damage
CC signaling pathway; increased gamma-irradiation-induced phosphorylation
CC of histone H2AX, and induces damage foci formation. Increases the
CC overall number of chromosomal abnormalities such as larger chromosomes
CC formation and multiple chromosomal fusions when overexpressed in gamma-
CC irradiated cells. May play a role in promoting lymphoid tumor
CC development: lymphoid cell lines overexpressing MCTS1 exhibit increased
CC growth rates and display increased protection against apoptosis. May
CC contribute to the pathogenesis and progression of breast cancer via
CC promotion of angiogenesis through the decline of inhibitory
CC THBS1/thrombospondin-1, and inhibition of apoptosis. Involved in the
CC process of proteasome degradation to down-regulate Tumor suppressor
CC p53/TP53 in breast cancer cell; Positively regulates phosphorylation of
CC MAPK1 and MAPK3 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via PUA domain) with DENR. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DOMAIN: The PUA RNA-binding domain is critical for cap binding, but not
CC sufficient for translation enhancer function. MCT1 N-terminal region is
CC required to enhance translation possibly through interaction with other
CC proteins.
CC -!- PTM: Phosphorylation is critical for stabilization and promotion of
CC cell proliferation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCTS1 family. {ECO:0000305}.
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DR EMBL; BC112668; AAI12669.1; -; mRNA.
DR RefSeq; NP_001039468.1; NM_001046003.2.
DR AlphaFoldDB; Q2KIE4; -.
DR SMR; Q2KIE4; -.
DR STRING; 9913.ENSBTAP00000046356; -.
DR PaxDb; Q2KIE4; -.
DR PRIDE; Q2KIE4; -.
DR Ensembl; ENSBTAT00000049468; ENSBTAP00000046356; ENSBTAG00000034992.
DR GeneID; 508412; -.
DR KEGG; bta:508412; -.
DR CTD; 28985; -.
DR VEuPathDB; HostDB:ENSBTAG00000034992; -.
DR VGNC; VGNC:56930; MCTS1.
DR eggNOG; KOG2523; Eukaryota.
DR GeneTree; ENSGT00550000074964; -.
DR HOGENOM; CLU_090468_0_1_1; -.
DR InParanoid; Q2KIE4; -.
DR OrthoDB; 1257440at2759; -.
DR TreeFam; TF315123; -.
DR Proteomes; UP000009136; Chromosome X.
DR Bgee; ENSBTAG00000034992; Expressed in tongue muscle and 104 other tissues.
DR ExpressionAtlas; Q2KIE4; baseline.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEA:UniProtKB-KW.
DR GO; GO:0001731; P:formation of translation preinitiation complex; IBA:GO_Central.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR016437; MCT-1/Tma20.
DR InterPro; IPR041366; Pre-PUA.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR22798; PTHR22798; 1.
DR Pfam; PF17832; Pre-PUA; 1.
DR Pfam; PF01472; PUA; 1.
DR PIRSF; PIRSF005067; Tma_RNA-bind_prd; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cytoplasm; DNA damage; Growth regulation; Initiation factor;
KW Phosphoprotein; Protein biosynthesis; Reference proteome; Transcription;
KW Transcription regulation; Tumor suppressor.
FT CHAIN 1..181
FT /note="Malignant T-cell-amplified sequence 1"
FT /id="PRO_0000344785"
FT DOMAIN 92..171
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT MOD_RES 81
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULC4"
FT MOD_RES 118
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULC4"
SQ SEQUENCE 181 AA; 20555 MW; 2FC00C7A992E24EB CRC64;
MFKKFDEKEN VSNCIQLKTS VIKGIKNQLI EQFPGIEPWL NQIMPKKDPV KIVRCHEHIE
ILTVNGELLF FRQREGPFYP TLRLLHKYPF ILPHQQVDKG AIKFVLSGAN IMCPGLTSPG
AKLYPAAVDT IVAIMAEGKQ HALCVGVMKM SAEDIEKVNK GIGIENIHYL NDGLWHMKTY
K
