MCTS1_DROME
ID MCTS1_DROME Reviewed; 182 AA.
AC Q9W445;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Malignant T-cell-amplified sequence 1 homolog {ECO:0000305};
DE AltName: Full=Multiple copies in T-cell lymphoma 1 homolog {ECO:0000305};
DE Short=MCT-1 {ECO:0000303|PubMed:25043021};
GN Name=MCTS1 {ECO:0000312|FlyBase:FBgn0029833};
GN ORFNames=CG5941 {ECO:0000312|FlyBase:FBgn0029833};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF46114.1};
RN [1] {ECO:0000312|EMBL:AAF46114.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|EMBL:AAF46114.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93310.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93310.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAK93310.1};
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.;
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, INTERACTION WITH DENR, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP THR-82; THR-118; SER-119 AND THR-125.
RX PubMed=25043021; DOI=10.1038/nature13401;
RA Schleich S., Strassburger K., Janiesch P.C., Koledachkina T., Miller K.K.,
RA Haneke K., Cheng Y.S., Kuchler K., Stoecklin G., Duncan K.E., Teleman A.A.;
RT "DENR-MCT-1 promotes translation re-initiation downstream of uORFs to
RT control tissue growth.";
RL Nature 512:208-212(2014).
CC -!- FUNCTION: Regulates translation as part of a complex with DENR.
CC Specifically required for translational re-initiation in mRNAs
CC containing upstream open reading frames (uORFs). Not required for
CC standard translational initiation. Regulates expression of a subset of
CC gene products including mbc, InR and EcR.
CC {ECO:0000269|PubMed:25043021}.
CC -!- SUBUNIT: Interacts with DENR. {ECO:0000269|PubMed:25043021}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|PIRNR:PIRNR005067}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in pupal
CC lethality. The abdominal epidermis is soft and transparent whereas the
CC head and thorax appear normal. {ECO:0000269|PubMed:25043021}.
CC -!- SIMILARITY: Belongs to the MCTS1 family. {ECO:0000305}.
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DR EMBL; AE014298; AAF46114.1; -; Genomic_DNA.
DR EMBL; AE014298; AFH07255.1; -; Genomic_DNA.
DR EMBL; AY051886; AAK93310.1; -; mRNA.
DR RefSeq; NP_001245541.1; NM_001258612.2.
DR RefSeq; NP_572288.1; NM_132060.4.
DR AlphaFoldDB; Q9W445; -.
DR SMR; Q9W445; -.
DR IntAct; Q9W445; 3.
DR STRING; 7227.FBpp0070832; -.
DR PaxDb; Q9W445; -.
DR PRIDE; Q9W445; -.
DR DNASU; 31536; -.
DR EnsemblMetazoa; FBtr0070867; FBpp0070832; FBgn0029833.
DR EnsemblMetazoa; FBtr0305344; FBpp0293887; FBgn0029833.
DR GeneID; 31536; -.
DR KEGG; dme:Dmel_CG5941; -.
DR UCSC; CG5941-RA; d. melanogaster.
DR CTD; 28985; -.
DR FlyBase; FBgn0029833; MCTS1.
DR VEuPathDB; VectorBase:FBgn0029833; -.
DR eggNOG; KOG2523; Eukaryota.
DR GeneTree; ENSGT00550000074964; -.
DR HOGENOM; CLU_090468_0_1_1; -.
DR InParanoid; Q9W445; -.
DR OMA; HDHIEIL; -.
DR OrthoDB; 1257440at2759; -.
DR PhylomeDB; Q9W445; -.
DR BioGRID-ORCS; 31536; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 31536; -.
DR PRO; PR:Q9W445; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0029833; Expressed in adult Malpighian tubule (Drosophila) and 26 other tissues.
DR Genevisible; Q9W445; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IPI:FlyBase.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0001731; P:formation of translation preinitiation complex; IBA:GO_Central.
DR GO; GO:0006417; P:regulation of translation; IEA:UniProtKB-KW.
DR GO; GO:0002188; P:translation reinitiation; IDA:FlyBase.
DR InterPro; IPR016437; MCT-1/Tma20.
DR InterPro; IPR041366; Pre-PUA.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR22798; PTHR22798; 1.
DR Pfam; PF17832; Pre-PUA; 1.
DR Pfam; PF01472; PUA; 1.
DR PIRSF; PIRSF005067; Tma_RNA-bind_prd; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome; Translation regulation.
FT CHAIN 1..182
FT /note="Malignant T-cell-amplified sequence 1 homolog"
FT /evidence="ECO:0000305"
FT /id="PRO_0000432075"
FT DOMAIN 93..172
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT MUTAGEN 82
FT /note="T->A: No effect on translation regulation activity."
FT /evidence="ECO:0000269|PubMed:25043021"
FT MUTAGEN 118
FT /note="T->A: No effect on translation regulation activity.
FT Reduced translation regulation activity; when associated
FT with A-119."
FT /evidence="ECO:0000269|PubMed:25043021"
FT MUTAGEN 119
FT /note="S->A: Reduced translation regulation activity.
FT Further reduced translation regulation activity; when
FT associated with A-119."
FT /evidence="ECO:0000269|PubMed:25043021"
FT MUTAGEN 125
FT /note="T->A: No effect on translation regulation activity."
FT /evidence="ECO:0000269|PubMed:25043021"
SQ SEQUENCE 182 AA; 20348 MW; 75A805666D78A60B CRC64;
MFKKFEEKDS ISSIQQLKSS VQKGIRAKLL EAYPKLESHI DLILPKKDSY RIAKCHDHIE
LLLNGAGDQV FFRHRDGPWM PTLRLLHKFP YFVTMQQVDK GAIRFVLSGA NVMCPGLTSP
GACMTPADKD TVVAIMAEGK EHALAVGLLT LSTQEILAKN KGIGIETYHF LNDGLWKSKP
VK
