MCTS1_RAT
ID MCTS1_RAT Reviewed; 182 AA.
AC Q4G009;
DT 22-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Malignant T-cell-amplified sequence 1;
DE Short=MCT-1;
GN Name=Mcts1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Anti-oncogene that plays a role in cell cycle regulation;
CC decreases cell doubling time and anchorage-dependent growth; shortens
CC the duration of G1 transit time and G1/S transition. When
CC constitutively expressed, increases CDK4 and CDK6 kinases activity and
CC CCND1/cyclin D1 protein level, as well as G1 cyclin/CDK complex
CC formation. Plays a role as translation enhancer; Recruits the density-
CC regulated protein/DENR and binds to the cap complex of the 5'-terminus
CC of mRNAs, subsequently altering the mRNA translation profile; Up-
CC regulates protein levels of BCL2L2, TFDP1, MRE11, CCND1 and E2F1, while
CC mRNA levels remains constant. Hyperactivates DNA damage signaling
CC pathway; increased gamma-irradiation-induced phosphorylation of histone
CC H2AX, and induces damage foci formation. Increases the overall number
CC of chromosomal abnormalities such as larger chromosomes formation and
CC multiple chromosomal fusions when overexpressed in gamma-irradiated
CC cells. May play a role in promoting lymphoid tumor development:
CC lymphoid cell lines overexpressing MCTS1 exhibit increased growth rates
CC and display increased protection against apoptosis. May contribute to
CC the pathogenesis and progression of breast cancer via promotion of
CC angiogenesis through the decline of inhibitory THBS1/thrombospondin-1,
CC and inhibition of apoptosis. Involved in the process of proteasome
CC degradation to down-regulate Tumor suppressor p53/TP53 in breast cancer
CC cell; Positively regulates phosphorylation of MAPK1 and MAPK3 (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts (via PUA domain) with DENR. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DOMAIN: The PUA RNA-binding domain is critical for cap binding, but not
CC sufficient for translation enhancer function. MCT1 N-terminal region is
CC required to enhance translation possibly through interaction with other
CC proteins.
CC -!- PTM: Phosphorylation is critical for stabilization and promotion of
CC cell proliferation. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MCTS1 family. {ECO:0000305}.
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DR EMBL; BC098848; AAH98848.1; -; mRNA.
DR RefSeq; NP_001037702.1; NM_001044237.1.
DR AlphaFoldDB; Q4G009; -.
DR SMR; Q4G009; -.
DR BioGRID; 257211; 1.
DR STRING; 10116.ENSRNOP00000061717; -.
DR iPTMnet; Q4G009; -.
DR PhosphoSitePlus; Q4G009; -.
DR jPOST; Q4G009; -.
DR PaxDb; Q4G009; -.
DR PRIDE; Q4G009; -.
DR Ensembl; ENSRNOT00000066677; ENSRNOP00000061717; ENSRNOG00000002563.
DR GeneID; 302500; -.
DR KEGG; rno:302500; -.
DR UCSC; RGD:1566390; rat.
DR CTD; 28985; -.
DR RGD; 1566390; Mcts1.
DR eggNOG; KOG2523; Eukaryota.
DR GeneTree; ENSGT00550000074964; -.
DR InParanoid; Q4G009; -.
DR OMA; HDHIEIL; -.
DR OrthoDB; 1257440at2759; -.
DR PhylomeDB; Q4G009; -.
DR TreeFam; TF315123; -.
DR PRO; PR:Q4G009; -.
DR Proteomes; UP000002494; Chromosome X.
DR Bgee; ENSRNOG00000002563; Expressed in pancreas and 20 other tissues.
DR ExpressionAtlas; Q4G009; baseline and differential.
DR Genevisible; Q4G009; RN.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEA:UniProtKB-KW.
DR GO; GO:0001731; P:formation of translation preinitiation complex; ISO:RGD.
DR GO; GO:0075522; P:IRES-dependent viral translational initiation; ISO:RGD.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR GO; GO:0032790; P:ribosome disassembly; ISO:RGD.
DR InterPro; IPR016437; MCT-1/Tma20.
DR InterPro; IPR041366; Pre-PUA.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR22798; PTHR22798; 1.
DR Pfam; PF17832; Pre-PUA; 1.
DR Pfam; PF01472; PUA; 1.
DR PIRSF; PIRSF005067; Tma_RNA-bind_prd; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cytoplasm; DNA damage; Growth regulation; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Tumor suppressor.
FT CHAIN 1..182
FT /note="Malignant T-cell-amplified sequence 1"
FT /id="PRO_0000344789"
FT DOMAIN 93..172
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT MOD_RES 82
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULC4"
FT MOD_RES 119
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ULC4"
SQ SEQUENCE 182 AA; 20550 MW; 0ED704B1C7602954 CRC64;
MGKGRFDEKE NVSNCIQLKT SVIKGIKNQL LEQFPGIEPW LNQIMPKKDP VKIVRCHEHI
EILTVNGELL FFRQREGPFY PTLRLLHKYP FILPHQQVDK GAIKFVLSGA NIMCPGLTSP
GAKLYPAAVD TIVAIMAEGK QHALCVGVMK MSAEDIEKVN KGIGIENIHY LNDGLWHMKT
YK
