ID   MCTX1_MILDI             Reviewed;         222 AA.
AC   A8QZJ5;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Millepora cytotoxin-1;
DE            Short=MCTx-1;
DE   Flags: Precursor;
OS   Millepora dichotoma (Net fire coral).
OC   Eukaryota; Metazoa; Cnidaria; Hydrozoa; Hydroidolina; Anthoathecata;
OC   Capitata; Milleporidae; Millepora.
OX   NCBI_TaxID=544484;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 76-85; 97-104; 164-173;
RP   177-184 AND 200-215, FUNCTION, SUBCELLULAR LOCATION, AND TOXIC DOSE.
RC   TISSUE=Nematoblast;
RX   PubMed=17983598; DOI=10.1016/j.bbrc.2007.10.153;
RA   Iguchi A., Iwanaga S., Nagai H.;
RT   "Isolation and characterization of a novel protein toxin from fire coral.";
RL   Biochem. Biophys. Res. Commun. 365:107-112(2008).
CC   -!- FUNCTION: Is potently cytotoxic (EC(50) value 79 ng/mL) towards L1210
CC       mouse leukemia cells, has hemagglutination activity on sheep
CC       erythrocytes, and is lethal in crayfish. Has no phospholipase A2
CC       activity. {ECO:0000269|PubMed:17983598}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17983598}.
CC       Nematocyst {ECO:0000269|PubMed:17983598}.
CC   -!- PTM: Is not glycosylated.
CC   -!- TOXIC DOSE: LD(50) is 106 mu/kg in crayfish.
CC       {ECO:0000269|PubMed:17983598}.
CC   -!- SIMILARITY: Belongs to the dermatopontin family. {ECO:0000305}.
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DR   EMBL; AB299385; BAF91371.1; -; mRNA.
DR   AlphaFoldDB; A8QZJ5; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   InterPro; IPR026645; Dermatopontin_fam.
DR   PANTHER; PTHR15040; PTHR15040; 1.
DR   Pfam; PF14704; DERM; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Nematocyst; Repeat; Secreted;
KW   Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..75
FT                   /evidence="ECO:0000269|PubMed:17983598"
FT                   /id="PRO_0000359432"
FT   CHAIN           76..222
FT                   /note="Millepora cytotoxin-1"
FT                   /id="PRO_0000359433"
FT   REPEAT          100..109
FT   REPEAT          153..162
FT   REPEAT          206..215
FT   DISULFID        89..115
FT                   /evidence="ECO:0000250"
FT   DISULFID        142..168
FT                   /evidence="ECO:0000250"
FT   DISULFID        179..222
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   222 AA;  25665 MW;  5939F98BD4C564A6 CRC64;
     MVTLYLHVPI LLLVVITARA APKPDTHNPF DELSSVAEKQ DLHYGDRSRK DPFIAQNDVG
     NNFRDGTQEN LTKVRSKVNQ YDQPFTFKCP LGETIKSIGS IHDNHYEDRQ WDIDCKPAGY
     TMGISTWSPY ANDYDGSMNF ECNEGSVVTG MSSIHDNYYE DRRYQLMCSY LNNWKRGSCA
     WTSYTTYDAS FVELTPTGKF LVGMKSQHNN YYEDRKFKML YC