MCU1_ARATH
ID MCU1_ARATH Reviewed; 338 AA.
AC Q1PE15; A0MFC5; O23200;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Calcium uniporter protein 1, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At4g36820 {ECO:0000312|Araport:AT4G36820};
GN ORFNames=AP22.5 {ECO:0000312|EMBL:CAB80348.1},
GN C7A10.540 {ECO:0000312|EMBL:CAB16819.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP GENE FAMILY, AND REVIEW.
RX PubMed=22200666; DOI=10.1093/jxb/err394;
RA Stael S., Wurzinger B., Mair A., Mehlmer N., Vothknecht U.C., Teige M.;
RT "Plant organellar calcium signalling: an emerging field.";
RL J. Exp. Bot. 63:1525-1542(2012).
CC -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates
CC calcium uptake into mitochondria. Constitutes a pore-forming and
CC calcium-conducting subunit. Mitochondrial calcium homeostasis plays key
CC roles in cellular physiology and regulates cell bioenergetics,
CC cytoplasmic calcium signals and activation of cell death pathways.
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8NE86}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28670.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB16819.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80348.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z99708; CAB16819.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161590; CAB80348.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE86706.1; -; Genomic_DNA.
DR EMBL; DQ446903; ABE66118.1; -; mRNA.
DR EMBL; DQ653251; ABK28670.1; ALT_SEQ; mRNA.
DR PIR; G85434; G85434.
DR RefSeq; NP_195400.2; NM_119846.4.
DR AlphaFoldDB; Q1PE15; -.
DR SMR; Q1PE15; -.
DR BioGRID; 15116; 9.
DR IntAct; Q1PE15; 8.
DR STRING; 3702.AT4G36820.1; -.
DR TCDB; 1.A.77.1.2; the mg(2+)/ca(2+) uniporter (mcu) family.
DR PaxDb; Q1PE15; -.
DR PRIDE; Q1PE15; -.
DR EnsemblPlants; AT4G36820.1; AT4G36820.1; AT4G36820.
DR GeneID; 829835; -.
DR Gramene; AT4G36820.1; AT4G36820.1; AT4G36820.
DR KEGG; ath:AT4G36820; -.
DR Araport; AT4G36820; -.
DR TAIR; locus:2115205; AT4G36820.
DR eggNOG; KOG2966; Eukaryota.
DR HOGENOM; CLU_066330_0_0_1; -.
DR InParanoid; Q1PE15; -.
DR OMA; CGLRRFM; -.
DR OrthoDB; 1076768at2759; -.
DR PhylomeDB; Q1PE15; -.
DR PRO; PR:Q1PE15; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q1PE15; baseline and differential.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0015292; F:uniporter activity; IBA:GO_Central.
DR GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR InterPro; IPR006769; MCU_C.
DR InterPro; IPR039055; MCU_fam.
DR PANTHER; PTHR13462; PTHR13462; 1.
DR Pfam; PF04678; MCU; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium channel; Calcium transport; Ion channel; Ion transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..36
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 37..338
FT /note="Calcium uniporter protein 1, mitochondrial"
FT /id="PRO_0000431370"
FT TRANSMEM 233..253
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..280
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 338 AA; 38349 MW; 5DE85990FC0D3C04 CRC64;
MVMMKKLLSN RLFNMSKTAS QSLMNCRTSS SSSLAMRTRV PKDIGEATID PEPGDLTISQ
RFLNKFSMNG IDTTSKMSIG ESLMEKLKEM DMNKDRIRLD GLSHPKEETL GLTVQDVKKL
LRAAEIEVIK TKLMETGKIW IRYSDFLGVC SDSSLDPSQG ALIAKMLDDS GNVIVMGNSV
CLRPHQLTKS IEGLLPLSQI HNPNDPRRKE LNELEAIKTV IDQKAHSLVR RELWAGLGYL
IIQTAGFMRL TFWDLTWDVM EPICFYVSSV YFMAGYTFFL KTSREPSFQG FYQSRFEAKQ
RKLMQSEDFD VGRYDELKKL FNPKPSGAVP KILGSLQN
