ID   MCU2_ARATH              Reviewed;         336 AA.
AC   O64823; Q8H1L7;
DT   26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   25-MAY-2022, entry version 114.
DE   RecName: Full=Calcium uniporter protein 2, mitochondrial;
DE   Flags: Precursor;
GN   OrderedLocusNames=At2g23790 {ECO:0000312|Araport:AT2G23790};
GN   ORFNames=F27L4.3 {ECO:0000312|EMBL:AAC17084.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=12481096; DOI=10.1104/pp.010207;
RA   Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT   "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT   of Arabidopsis.";
RL   Plant Physiol. 130:2118-2128(2002).
RN   [4]
RP   GENE FAMILY, AND REVIEW.
RX   PubMed=22200666; DOI=10.1093/jxb/err394;
RA   Stael S., Wurzinger B., Mair A., Mehlmer N., Vothknecht U.C., Teige M.;
RT   "Plant organellar calcium signalling: an emerging field.";
RL   J. Exp. Bot. 63:1525-1542(2012).
CC   -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates
CC       calcium uptake into mitochondria. Constitutes a pore-forming and
CC       calcium-conducting subunit. Mitochondrial calcium homeostasis plays key
CC       roles in cellular physiology and regulates cell bioenergetics,
CC       cytoplasmic calcium signals and activation of cell death pathways.
CC       {ECO:0000250|UniProtKB:Q8NE86}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q8NE86}; Multi-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q8NE86}.
CC   -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC004482; AAC17084.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07491.1; -; Genomic_DNA.
DR   EMBL; AY145900; AAN59955.1; -; mRNA.
DR   PIR; A84629; A84629.
DR   RefSeq; NP_179959.1; NM_127942.3.
DR   AlphaFoldDB; O64823; -.
DR   SMR; O64823; -.
DR   BioGRID; 2263; 4.
DR   IntAct; O64823; 4.
DR   STRING; 3702.AT2G23790.1; -.
DR   PaxDb; O64823; -.
DR   PRIDE; O64823; -.
DR   EnsemblPlants; AT2G23790.1; AT2G23790.1; AT2G23790.
DR   GeneID; 816911; -.
DR   Gramene; AT2G23790.1; AT2G23790.1; AT2G23790.
DR   KEGG; ath:AT2G23790; -.
DR   Araport; AT2G23790; -.
DR   TAIR; locus:2049013; AT2G23790.
DR   eggNOG; KOG2966; Eukaryota.
DR   HOGENOM; CLU_066330_0_0_1; -.
DR   InParanoid; O64823; -.
DR   OMA; EIMQMPV; -.
DR   OrthoDB; 1076768at2759; -.
DR   PhylomeDB; O64823; -.
DR   PRO; PR:O64823; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; O64823; baseline and differential.
DR   GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR   GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR   GO; GO:0015292; F:uniporter activity; IBA:GO_Central.
DR   GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR   GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR   InterPro; IPR006769; MCU_C.
DR   InterPro; IPR039055; MCU_fam.
DR   PANTHER; PTHR13462; PTHR13462; 1.
DR   Pfam; PF04678; MCU; 1.
PE   2: Evidence at transcript level;
KW   Calcium; Calcium channel; Calcium transport; Ion channel; Ion transport;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW   Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..69
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           70..336
FT                   /note="Calcium uniporter protein 2, mitochondrial"
FT                   /id="PRO_0000431371"
FT   TRANSMEM        231..251
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   336 AA;  38177 MW;  1F448CDE9D3E7618 CRC64;
     MAMRKLLSKK LFNITNVASQ SLMNCRISSS SLAVRTRVPN DSTDTTKIAP EPGDLAMSRR
     FMHNSAMIRP AEIMQMPVGE SLIEKLREID GSKDRIRLDG LSPPERETSL TVADTKKLLR
     AAQIEIVKSK LRETGRSWMP YKEFVSVCGE ASSDPDLGSK IAKMLDDSAN VIVLGDSVCI
     RPDQVTKSIE GLLPLPKIHN PNDPRRIEFK ELEAEKAVID VKAHTLVRKE LWAGLGYLIL
     QTAGFMRLTF WELSWDVMEP ICFYVTSVYF MAGYAFFLRT SKEPSFEGFY QSRFEAKQRK
     LMNEYEFDLE RYNELKKLFC SKPSDHVSKI LGAIKS