MCU3_ARATH
ID MCU3_ARATH Reviewed; 321 AA.
AC Q9LVR5; Q94B21;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Calcium uniporter protein 3, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At5g66650 {ECO:0000312|Araport:AT5G66650};
GN ORFNames=MSN2.3 {ECO:0000312|EMBL:BAA97271.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND REVIEW.
RX PubMed=22200666; DOI=10.1093/jxb/err394;
RA Stael S., Wurzinger B., Mair A., Mehlmer N., Vothknecht U.C., Teige M.;
RT "Plant organellar calcium signalling: an emerging field.";
RL J. Exp. Bot. 63:1525-1542(2012).
CC -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates
CC calcium uptake into mitochondria. Constitutes a pore-forming and
CC calcium-conducting subunit. Mitochondrial calcium homeostasis plays key
CC roles in cellular physiology and regulates cell bioenergetics,
CC cytoplasmic calcium signals and activation of cell death pathways.
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8NE86}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. {ECO:0000305}.
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DR EMBL; AB018119; BAA97271.1; -; Genomic_DNA.
DR EMBL; CP002688; AED98245.1; -; Genomic_DNA.
DR EMBL; AY042903; AAK68843.1; -; mRNA.
DR EMBL; BT020326; AAV85681.1; -; mRNA.
DR RefSeq; NP_201466.1; NM_126063.3.
DR AlphaFoldDB; Q9LVR5; -.
DR BioGRID; 22039; 4.
DR IntAct; Q9LVR5; 4.
DR STRING; 3702.AT5G66650.1; -.
DR PaxDb; Q9LVR5; -.
DR PRIDE; Q9LVR5; -.
DR ProteomicsDB; 238328; -.
DR EnsemblPlants; AT5G66650.1; AT5G66650.1; AT5G66650.
DR GeneID; 836797; -.
DR Gramene; AT5G66650.1; AT5G66650.1; AT5G66650.
DR KEGG; ath:AT5G66650; -.
DR Araport; AT5G66650; -.
DR TAIR; locus:2173629; AT5G66650.
DR eggNOG; KOG2966; Eukaryota.
DR HOGENOM; CLU_066330_0_0_1; -.
DR InParanoid; Q9LVR5; -.
DR OMA; CFYVTST; -.
DR OrthoDB; 1076768at2759; -.
DR PhylomeDB; Q9LVR5; -.
DR PRO; PR:Q9LVR5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVR5; baseline and differential.
DR Genevisible; Q9LVR5; AT.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR GO; GO:0005262; F:calcium channel activity; IDA:TAIR.
DR GO; GO:0015292; F:uniporter activity; IDA:TAIR.
DR GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR GO; GO:0070509; P:calcium ion import; IMP:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR InterPro; IPR006769; MCU_C.
DR InterPro; IPR039055; MCU_fam.
DR PANTHER; PTHR13462; PTHR13462; 1.
DR Pfam; PF04678; MCU; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium channel; Calcium transport; Ion channel; Ion transport;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..56
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 57..321
FT /note="Calcium uniporter protein 3, mitochondrial"
FT /id="PRO_0000431372"
FT TRANSMEM 223..243
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 249..269
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT CONFLICT 94
FT /note="K -> E (in Ref. 3; AAK68843)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 321 AA; 36766 MW; 2E24E4E0A4A613D0 CRC64;
MSSKKSLVQS LFNISKTYSR ISGLTRMRPT KSGGIPPDAG DSGIRRRFLH KRAFFSPEIV
PKGGNLMEKL RELTLSNNNR IRLDEMLPPP SPKKSSPEFF PAVTVEDVKK LMRAAEMELV
KSKLREIGKN WVPYSEFVRV CGEYSSDPEQ GNRVANMLDE AGNVIVLGKL VCLKPEELTS
AMAGLIPTLE PSLDAETRQE FEQLEIIKSD IDKRADDLVR KELWAGLGLI MAQTVGFFRL
TFWELSWDVM EPICFYVTST YFMAGYAFFL RTSKEPSFEG FYKSRFETKQ KRLIKMLDFD
IDRFTKLQKM HRPNLTKSGR C
