MCU5_ARATH
ID MCU5_ARATH Reviewed; 293 AA.
AC Q8VYR0; Q9FVU1;
DT 26-NOV-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Calcium uniporter protein 5, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At1g57610 {ECO:0000312|Araport:AT1G57610};
GN ORFNames=T8L23.8 {ECO:0000312|EMBL:AAG50752.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAL49777.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND REVIEW.
RX PubMed=22200666; DOI=10.1093/jxb/err394;
RA Stael S., Wurzinger B., Mair A., Mehlmer N., Vothknecht U.C., Teige M.;
RT "Plant organellar calcium signalling: an emerging field.";
RL J. Exp. Bot. 63:1525-1542(2012).
CC -!- FUNCTION: Mitochondrial inner membrane calcium uniporter that mediates
CC calcium uptake into mitochondria. Constitutes a pore-forming and
CC calcium-conducting subunit. Mitochondrial calcium homeostasis plays key
CC roles in cellular physiology and regulates cell bioenergetics,
CC cytoplasmic calcium signals and activation of cell death pathways.
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q8NE86}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q8NE86}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8VYR0-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the MCU (TC 1.A.77) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG50752.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC079733; AAG50752.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE33441.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33442.1; -; Genomic_DNA.
DR EMBL; AY070082; AAL49777.1; -; mRNA.
DR PIR; C96610; C96610.
DR RefSeq; NP_176074.2; NM_104558.4. [Q8VYR0-1]
DR RefSeq; NP_974043.1; NM_202314.3. [Q8VYR0-1]
DR AlphaFoldDB; Q8VYR0; -.
DR SMR; Q8VYR0; -.
DR STRING; 3702.AT1G57610.1; -.
DR PaxDb; Q8VYR0; -.
DR PRIDE; Q8VYR0; -.
DR ProteomicsDB; 238245; -. [Q8VYR0-1]
DR EnsemblPlants; AT1G57610.1; AT1G57610.1; AT1G57610. [Q8VYR0-1]
DR EnsemblPlants; AT1G57610.2; AT1G57610.2; AT1G57610. [Q8VYR0-1]
DR GeneID; 842137; -.
DR Gramene; AT1G57610.1; AT1G57610.1; AT1G57610. [Q8VYR0-1]
DR Gramene; AT1G57610.2; AT1G57610.2; AT1G57610. [Q8VYR0-1]
DR KEGG; ath:AT1G57610; -.
DR Araport; AT1G57610; -.
DR TAIR; locus:2206510; AT1G57610.
DR eggNOG; KOG2966; Eukaryota.
DR HOGENOM; CLU_066330_1_0_1; -.
DR InParanoid; Q8VYR0; -.
DR OMA; ILAHKQV; -.
DR OrthoDB; 1076768at2759; -.
DR PhylomeDB; Q8VYR0; -.
DR PRO; PR:Q8VYR0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VYR0; baseline and differential.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:1990246; C:uniplex complex; IBA:GO_Central.
DR GO; GO:0005262; F:calcium channel activity; IBA:GO_Central.
DR GO; GO:0015292; F:uniporter activity; IBA:GO_Central.
DR GO; GO:0036444; P:calcium import into the mitochondrion; IBA:GO_Central.
DR GO; GO:0051560; P:mitochondrial calcium ion homeostasis; IBA:GO_Central.
DR InterPro; IPR006769; MCU_C.
DR InterPro; IPR039055; MCU_fam.
DR PANTHER; PTHR13462; PTHR13462; 1.
DR Pfam; PF04678; MCU; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Calcium channel; Calcium transport;
KW Ion channel; Ion transport; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..33
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 34..293
FT /note="Calcium uniporter protein 5, mitochondrial"
FT /id="PRO_0000431374"
FT TRANSMEM 168..188
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 198..218
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 293 AA; 33685 MW; 3F599D18C29038D7 CRC64;
MWSVMGLVRR TAMSSTVNKA SPVRSLLGGF RCLNVESKEE DEKKDMTVLE AKKLMRLVNV
EDMKKKLIGM GDKEMVTYTT LIEASQGLGI ARSLDEAHAF ARVLDDAGVI LIFRDKVYLH
PHKVVDLIRK AVPLGLNPDD ELIREEFDKM RSMKEEIDVL AHQQVRKILW GGLGYSVVQI
GIFVRLTFWE FSWDVMEPIT FFTTATGIIV GYAYFLMTSR DPTYQDFMKR LFLSRQRKLL
KSHKFDAERF KELENKWKIT SCSSSSCHAN ASIRNRVGVD LDLEDSLQSH HRD
