MD10A_ARATH
ID MD10A_ARATH Reviewed; 186 AA.
AC Q9FHZ2;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 10a {ECO:0000303|PubMed:17560376};
GN Name=MED10A {ECO:0000303|PubMed:17560376};
GN Synonyms=MED10_2 {ECO:0000303|PubMed:22021418};
GN OrderedLocusNames=At5g41910 {ECO:0000312|Araport:AT5G41910};
GN ORFNames=MJC20.1 {ECO:0000312|EMBL:BAB08424.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, SUBUNIT, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [6]
RP SUBUNIT, MUTAGENESIS OF CYS-88 AND CYS-118, AND INTERACTION WITH GEBPL.
RX PubMed=25877331; DOI=10.1042/bj20150132;
RA Shaikhali J., Davoine C., Braennstroem K., Rouhier N., Bygdell J.,
RA Bjoerklund S., Wingsle G.;
RT "Biochemical and redox characterization of the mediator complex and its
RT associated transcription factor GeBPL, a GLABROUS1 enhancer binding
RT protein.";
RL Biochem. J. 468:385-400(2015).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional pre-initiation complex with RNA polymerase II and the
CC general transcription factors. {ECO:0000305|PubMed:17560376,
CC ECO:0000305|PubMed:22021418}.
CC -!- SUBUNIT: Mono-, di- and oligomers (PubMed:25877331). Component of the
CC Mediator complex (PubMed:17560376, PubMed:22021418). Interacts with
CC GEBPL (PubMed:25877331). {ECO:0000269|PubMed:17560376,
CC ECO:0000269|PubMed:22021418, ECO:0000269|PubMed:25877331}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC {ECO:0000305}.
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DR EMBL; AB017067; BAB08424.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94743.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69466.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69467.1; -; Genomic_DNA.
DR EMBL; BT002847; AAO22665.1; -; mRNA.
DR EMBL; BT004431; AAO42425.1; -; mRNA.
DR RefSeq; NP_001331137.1; NM_001344426.1.
DR RefSeq; NP_001331138.1; NM_001344425.1.
DR RefSeq; NP_199006.1; NM_123556.3.
DR AlphaFoldDB; Q9FHZ2; -.
DR SMR; Q9FHZ2; -.
DR BioGRID; 19447; 3.
DR IntAct; Q9FHZ2; 9.
DR STRING; 3702.AT5G41910.1; -.
DR PaxDb; Q9FHZ2; -.
DR PRIDE; Q9FHZ2; -.
DR ProteomicsDB; 250835; -.
DR DNASU; 834196; -.
DR EnsemblPlants; AT5G41910.1; AT5G41910.1; AT5G41910.
DR EnsemblPlants; AT5G41910.2; AT5G41910.2; AT5G41910.
DR EnsemblPlants; AT5G41910.3; AT5G41910.3; AT5G41910.
DR GeneID; 834196; -.
DR Gramene; AT5G41910.1; AT5G41910.1; AT5G41910.
DR Gramene; AT5G41910.2; AT5G41910.2; AT5G41910.
DR Gramene; AT5G41910.3; AT5G41910.3; AT5G41910.
DR KEGG; ath:AT5G41910; -.
DR Araport; AT5G41910; -.
DR TAIR; locus:2165680; AT5G41910.
DR eggNOG; KOG3046; Eukaryota.
DR HOGENOM; CLU_096169_2_0_1; -.
DR InParanoid; Q9FHZ2; -.
DR OMA; YSKHCME; -.
DR OrthoDB; 1403581at2759; -.
DR PhylomeDB; Q9FHZ2; -.
DR PRO; PR:Q9FHZ2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FHZ2; baseline and differential.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019145; Mediator_Med10.
DR Pfam; PF09748; Med10; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..186
FT /note="Mediator of RNA polymerase II transcription subunit
FT 10a"
FT /id="PRO_0000418114"
FT MUTAGEN 88
FT /note="C->S: Loss of oligomerization. Loss of
FT oligomerization and dimerization; when associated with S-
FT 118."
FT /evidence="ECO:0000269|PubMed:25877331"
FT MUTAGEN 118
FT /note="C->S: Loss of oligomerization. Loss of
FT oligomerization and dimerization; when associated with S-
FT 88."
FT /evidence="ECO:0000269|PubMed:25877331"
SQ SEQUENCE 186 AA; 20727 MW; F0D36D9DAC5DC160 CRC64;
MDQTQNTIAG IGEINGSIKT EINAIATVDD SNEKLNQIIN SNQKILELLH QLKLTVSSFT
PASQLHLLQR LNSLVMELDN MAKLSDKCNI QVPIEVLNLI DDGKNPDEFT KDVLNKNCIA
KNQVTKGKSD AFKGLRKHLL EELEQAFPDE VDRYRDIRAS YAAEAKRLAQ TQSVLPNGDA
KVKSEL
