MD10B_ARATH
ID MD10B_ARATH Reviewed; 189 AA.
AC F4HPA7; Q93WN1; Q9LQY6;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 10b;
GN Name=MED10B; Synonyms=MED10_1; OrderedLocusNames=At1g26665;
GN ORFNames=T24P13.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, SUBUNIT, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376, ECO:0000269|PubMed:22021418}.
CC -!- INTERACTION:
CC F4HPA7; Q94AI7: TPL; NbExp=3; IntAct=EBI-1386239, EBI-2119299;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4HPA7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4HPA7-2; Sequence=VSP_043990;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 10 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF87025.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC006535; AAF87025.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30717.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30718.1; -; Genomic_DNA.
DR EMBL; AF372912; AAK49628.1; -; mRNA.
DR EMBL; AY057733; AAL15363.1; -; mRNA.
DR PIR; F86393; F86393.
DR RefSeq; NP_564254.1; NM_102429.4. [F4HPA7-2]
DR RefSeq; NP_973916.1; NM_202187.2. [F4HPA7-1]
DR AlphaFoldDB; F4HPA7; -.
DR SMR; F4HPA7; -.
DR IntAct; F4HPA7; 3.
DR STRING; 3702.AT1G26665.1; -.
DR PaxDb; F4HPA7; -.
DR PRIDE; F4HPA7; -.
DR ProteomicsDB; 238766; -. [F4HPA7-1]
DR EnsemblPlants; AT1G26665.1; AT1G26665.1; AT1G26665. [F4HPA7-2]
DR EnsemblPlants; AT1G26665.2; AT1G26665.2; AT1G26665. [F4HPA7-1]
DR GeneID; 839207; -.
DR Gramene; AT1G26665.1; AT1G26665.1; AT1G26665. [F4HPA7-2]
DR Gramene; AT1G26665.2; AT1G26665.2; AT1G26665. [F4HPA7-1]
DR KEGG; ath:AT1G26665; -.
DR Araport; AT1G26665; -.
DR TAIR; locus:505006147; AT1G26665.
DR eggNOG; KOG3046; Eukaryota.
DR InParanoid; F4HPA7; -.
DR OMA; HQLYLTI; -.
DR OrthoDB; 1403581at2759; -.
DR PhylomeDB; F4HPA7; -.
DR PRO; PR:F4HPA7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4HPA7; baseline and differential.
DR Genevisible; F4HPA7; AT.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019145; Mediator_Med10.
DR Pfam; PF09748; Med10; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..189
FT /note="Mediator of RNA polymerase II transcription subunit
FT 10b"
FT /id="PRO_0000418115"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 167..168
FT /note="VT -> EA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_043990"
SQ SEQUENCE 189 AA; 20913 MW; C654B120A63A0FAC CRC64;
MDPTQNTSAG IGGSNGTIRY QTNDGTSTVT VADDSKENLS QVINSIEKTL GVLHQLHLTV
TSFTPASQLH LLQRLNSLVM ELDNMTKLSE KCNIQIPMEV LNLIDDGKNP DEFTKDVLNS
CIARNQVTKG KTDAFKDLRK HILEELEQNF PDEVDMYREI RASSAAVTKR LAQSQSVLPN
GDAKVKNEL
