MD12L_MOUSE
ID MD12L_MOUSE Reviewed; 2157 AA.
AC Q8BQM9; Q6ZPF2; Q8C9E3;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 12-like protein;
DE AltName: Full=Mediator complex subunit 12-like protein;
GN Name=Med12l; Synonyms=Kiaa3007;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC TISSUE=Embryonic tail;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 1-751 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Corpus striatum, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP PROTEIN SEQUENCE OF 162-174, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
CC -!- FUNCTION: May be a component of the Mediator complex, a coactivator
CC involved in the regulated transcription of nearly all RNA polymerase
CC II-dependent genes. Mediator functions as a bridge to convey
CC information from gene-specific regulatory proteins to the basal RNA
CC polymerase II transcription machinery. Mediator is recruited to
CC promoters by direct interactions with regulatory proteins and serves as
CC a scaffold for the assembly of a functional preinitiation complex with
CC RNA polymerase II and the general transcription factors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: May be a component of the Mediator complex, which is known to
CC be composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12,
CC MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21,
CC MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC,
CC CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a
CC distinct module termed the CDK8 module. Mediator containing the CDK8
CC module is less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8BQM9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BQM9-2; Sequence=VSP_029999;
CC Name=3;
CC IsoId=Q8BQM9-3; Sequence=VSP_029998, VSP_030000, VSP_030003;
CC Name=4;
CC IsoId=Q8BQM9-4; Sequence=VSP_029998, VSP_030001, VSP_030002;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 12 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC98285.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK129475; BAC98285.1; ALT_INIT; mRNA.
DR EMBL; AK042296; BAC31219.1; -; mRNA.
DR EMBL; AK047743; BAC33144.1; -; mRNA.
DR EMBL; AC115919; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC122038; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_808523.2; NM_177855.3.
DR AlphaFoldDB; Q8BQM9; -.
DR BioGRID; 236806; 1.
DR STRING; 10090.ENSMUSP00000127038; -.
DR iPTMnet; Q8BQM9; -.
DR PhosphoSitePlus; Q8BQM9; -.
DR MaxQB; Q8BQM9; -.
DR PaxDb; Q8BQM9; -.
DR PeptideAtlas; Q8BQM9; -.
DR PRIDE; Q8BQM9; -.
DR ProteomicsDB; 293438; -. [Q8BQM9-1]
DR ProteomicsDB; 293439; -. [Q8BQM9-2]
DR ProteomicsDB; 293440; -. [Q8BQM9-3]
DR ProteomicsDB; 293441; -. [Q8BQM9-4]
DR Antibodypedia; 46744; 25 antibodies from 14 providers.
DR Ensembl; ENSMUST00000029393; ENSMUSP00000029393; ENSMUSG00000056476. [Q8BQM9-3]
DR Ensembl; ENSMUST00000040325; ENSMUSP00000042269; ENSMUSG00000056476. [Q8BQM9-1]
DR Ensembl; ENSMUST00000040846; ENSMUSP00000041859; ENSMUSG00000056476. [Q8BQM9-3]
DR Ensembl; ENSMUST00000164225; ENSMUSP00000127038; ENSMUSG00000056476. [Q8BQM9-2]
DR GeneID; 329650; -.
DR KEGG; mmu:329650; -.
DR UCSC; uc008pig.1; mouse. [Q8BQM9-3]
DR UCSC; uc008pih.1; mouse. [Q8BQM9-4]
DR UCSC; uc008pii.2; mouse. [Q8BQM9-2]
DR CTD; 116931; -.
DR MGI; MGI:2139916; Med12l.
DR VEuPathDB; HostDB:ENSMUSG00000056476; -.
DR eggNOG; KOG3598; Eukaryota.
DR GeneTree; ENSGT00440000037505; -.
DR HOGENOM; CLU_000904_1_0_1; -.
DR InParanoid; Q8BQM9; -.
DR OrthoDB; 15873at2759; -.
DR PhylomeDB; Q8BQM9; -.
DR TreeFam; TF324178; -.
DR BioGRID-ORCS; 329650; 2 hits in 53 CRISPR screens.
DR ChiTaRS; Med12l; mouse.
DR PRO; PR:Q8BQM9; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8BQM9; protein.
DR Bgee; ENSMUSG00000056476; Expressed in substantia nigra and 181 other tissues.
DR ExpressionAtlas; Q8BQM9; baseline and differential.
DR Genevisible; Q8BQM9; MM.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0008013; F:beta-catenin binding; IEA:InterPro.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019035; Mediator_Med12.
DR InterPro; IPR021989; Mediator_Med12_catenin-bd.
DR InterPro; IPR021990; Mediator_Med12_LCEWAV.
DR Pfam; PF09497; Med12; 1.
DR Pfam; PF12145; Med12-LCEWAV; 1.
DR Pfam; PF12144; Med12-PQL; 1.
DR SMART; SM01281; Med12; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Direct protein sequencing; Nucleus;
KW Phosphoprotein; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..2157
FT /note="Mediator of RNA polymerase II transcription subunit
FT 12-like protein"
FT /id="PRO_0000313054"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1437..1461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1724..1807
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2040..2157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1437..1458
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1752..1783
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1784..1807
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2063..2157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 462
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q86YW9"
FT VAR_SEQ 132
FT /note="K -> KELPLAKSGVPQ (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:14621295,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_029998"
FT VAR_SEQ 656
FT /note="E -> EEHSVMARLGLGSGATHIFDDVDKSDFKSDPASEFP (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029999"
FT VAR_SEQ 716..745
FT /note="DESSSHECNQRTILLYGVGKERDEARHQLK -> VRHVSISVYHDDFIRPSV
FT IGCQIIHSLCLL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030000"
FT VAR_SEQ 716..721
FT /note="DESSSH -> GILAMW (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_030001"
FT VAR_SEQ 722..2157
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_030002"
FT VAR_SEQ 746..2157
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_030003"
SQ SEQUENCE 2157 AA; 241426 MW; 4385941E622D8B9B CRC64;
MAAFGLLSYE QRPLKRPRLG PPDVYPQDPK QKEDELTAVN VKQGFNNQPA FTGDEHGSAR
NIVINPSKIG AYFSSILAEK LKLNTFQDTG KKKPQVNAKD NYWLVTARSQ SAIHSWFSDL
AGNKPLAILA KKVPILSKKE DVFAYLAKYS VPMVRATWLI KMTCAYYSAI SEAKIKKRQA
PDPNLEWTQI STRYLREQLV KISDFYHMAS STGDGPVPVP PEVEQAMKQW EYNEKLAFHM
FQEGMLEKHE YLTWILDVLE KIRPVDDSLL KLLLPLMLQY SDEFVQSAYL SRRLAYFCAR
RLSLLLSDSP NLLAAHSPHM IIGTNNTSIG TPSPGTPGPG MSPVQLAFSD FLSCAQHGPL
VYGLSCMLQT VTLCCPSALV WNYSTNENKI SNPGSPLDLL QVAPSSLPMP GGNTAFNQQV
RARIYEVEQQ IKQRGRAVEV RWSFDKCQES TAGVTISRVL HTLEVLDRHC FDRTDSSNSM
ETLYHKIFWA NQNKDNQEVA PNDEAVVTLL CEWAVSCKRS GKHRAMAVAK LLEKRQAEIE
AERCGESEVL DEKESISSAS LAGSSLPVFQ NVLLRFLDTQ APALSDPNSE CEKVEFVNLV
LLFCEFIRHD VFSHDAYMCT LISRGDLSVT ASTGLRSPAG ENSDEHYSKD HDMKMEIFSP
MPGESCENIN PSLSRRLSVN GEKLLKREKP RELIFPSNYD LLRHLQYATH FPIPLDESSS
HECNQRTILL YGVGKERDEA RHQLKKITKD ILKILNKKGT TESGVGDEGQ KARKTKQEVF
PTPENVFTKL QLLSYFDQHQ VTSQISNNVL EQITSFASGT SYHLPLAHHI QLIFDLMEPA
LNINGLIDFA IQLLNELSVV EAELLLKSSS LAGSYTTGLC VCIVAVLRRY HSCLILNPDQ
TAQVFEGLCG VVKHVVNPSE CSSPERCILA YLYDLYVSCS HLRSKFGDLF SSACSKVKQT
IYNNVMPANS NLRWDPDFMM DFIENPSARS INYSMLGKIL NDNAANRYSF VCNTLMNVCM
GHQDAGRIND IANFSSELTA CCTVLSSEWL GVLKALCCSS NHVWGFNDVL CTVDVSDLSF
HDSLATFIAI LIARQCFSLE DVVQHVALPS LLAAACGDAD AEPGARMTCR LLLHLFRAPQ
ACFFPQGTGK PFPGIRSSCD RHLLAAAHNS IEVGAVFAVL KAIMMLGDAK IGSNNVNTMK
NEDFGMRGLR RDGNAEDAWA TSQNSKSYGK SISIETANLR EYARYVLRTI CQQEWVGEHC
LKEPERLCTD KELILDPVLS NMQAQKLLQL ICYPHGIKEC TEGDNLQRQH IKRILQNLEQ
WTLRQSWLEL QLMIKQCLKD PSSGSVAEMN NLLDNIAKAT IEVFQQSADL NNNASNSGMS
LFNPNTIGSV DPSSTRQNGI KTFLSSSERR GVWLVAPLIA RLPTSVQGRV LKAAGEELEK
GQHLGSSSKK ERDRQKQKSM SLLSQQPFLS LVLTCLKGQD EQREGLLTSL QNQVNQILSN
WREERYQDDT KARQMMHEAL QLRLNLVGGM FDTVQRSTQG TTDWALLLLQ IITSGTVDMH
TNNELFTTVL DMLGVLINGT LASDLSSASP GGSEENKRAY MNLVKKLKKE LGDKRSESID
KVRQLLPLPK QTCDVITCEP MGSLIDTKGN KIAGFDSIDK KQARGLQVST KQKVSPWDLF
EGQKNPAPLS WAWFGTVRVD RKVIKYEEQQ HFLLYHTHTM PKPRSYYLEP LPLPPEEEEE
ELTSPVSQEP ERKSAELSDQ GKATADEEKK TKGRKRKTKS SSRIDEYQQT NLYRVPPNYS
PMSSQMTHHP QPALWGYNLV SQPQQPSFFL QNPSLNPGGS RLDPAGSFVP TNTKQALSNM
LQRRSGAMLQ PPSLHAVTSQ QQLLQMKLLQ QQQQQQQQQQ QQQQRLLRQA QTRPFQQGQP
GDQAALFTAQ ARPSPQLPQY PGLQQAQTMP QGYTMYGTQM PLQQAAQQQP GGVVLSPSYN
SRAYPAAHSS PALMERLRQL QQQPSGYVQQ QASPYLQPVA GSQRLNHQAL QQSPLVGGGI
DAVLTPAHPN LPSVPLPQDP MRPRQQQVRQ QQRLLQVWGR EMQQPQQAPQ PQQPSQTQSQ
ALGLQAMQPQ QPLFPRQGLQ QTQQQQQTAA LVRQLQKQLS SNQPQQGVTP CAHPSHF
