MD13L_MOUSE
ID MD13L_MOUSE Reviewed; 2207 AA.
AC Q6JPI3; Q3TRF4; Q3UQI8; Q80TM3; Q80WQ0;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 13-like;
DE AltName: Full=Mediator complex subunit 13-like;
DE AltName: Full=Thyroid hormone receptor-associated protein 2;
DE AltName: Full=Thyroid hormone receptor-associated protein complex 240 kDa component-like;
GN Name=Med13l; Synonyms=Kiaa1025, Thrap2, Trap240l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=15145061; DOI=10.1016/j.gene.2004.02.044;
RA Musante L., Bartsch O., Ropers H.-H., Kalscheuer V.M.;
RT "cDNA cloning and characterization of the human THRAP2 gene which maps to
RT chromosome 12q24, and its mouse ortholog Thrap2.";
RL Gene 332:119-127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-465 AND 1846-2207.
RC STRAIN=NOD;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 550-2207.
RC TISSUE=Brain;
RX PubMed=12693553; DOI=10.1093/dnares/10.1.35;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S.,
RA Nakajima D., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: II.
RT The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:35-48(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1083-2207.
RC STRAIN=C57BL/6J; TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. This subunit may specifically
CC regulate transcription of targets of the Wnt signaling pathway and SHH
CC signaling pathway (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in heart and weakly expressed in
CC brain, spleen, lung, liver, kidney and testis.
CC {ECO:0000269|PubMed:15145061}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR08419.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY338464; AAR08419.1; ALT_FRAME; mRNA.
DR EMBL; AK142391; BAE25053.1; -; mRNA.
DR EMBL; AK162842; BAE37076.1; -; mRNA.
DR EMBL; AK122419; BAC65701.1; -; mRNA.
DR EMBL; BC052320; AAH52320.1; -; mRNA.
DR CCDS; CCDS57375.1; -.
DR RefSeq; NP_766012.3; NM_172424.4.
DR AlphaFoldDB; Q6JPI3; -.
DR BioGRID; 218020; 9.
DR STRING; 10090.ENSMUSP00000098379; -.
DR iPTMnet; Q6JPI3; -.
DR PhosphoSitePlus; Q6JPI3; -.
DR EPD; Q6JPI3; -.
DR MaxQB; Q6JPI3; -.
DR PaxDb; Q6JPI3; -.
DR PRIDE; Q6JPI3; -.
DR ProteomicsDB; 252756; -.
DR DNASU; 76199; -.
DR GeneID; 76199; -.
DR KEGG; mmu:76199; -.
DR CTD; 23389; -.
DR MGI; MGI:2670178; Med13l.
DR eggNOG; KOG3600; Eukaryota.
DR InParanoid; Q6JPI3; -.
DR PhylomeDB; Q6JPI3; -.
DR BioGRID-ORCS; 76199; 6 hits in 72 CRISPR screens.
DR ChiTaRS; Med13l; mouse.
DR PRO; PR:Q6JPI3; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q6JPI3; protein.
DR GO; GO:0016592; C:mediator complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IC:MGI.
DR InterPro; IPR009401; Med13_C.
DR InterPro; IPR021643; Mediator_Med13_N.
DR InterPro; IPR041285; MID_MedPIWI.
DR Pfam; PF06333; Med13_C; 1.
DR Pfam; PF11597; Med13_N; 1.
DR Pfam; PF18296; MID_MedPIWI; 1.
PE 1: Evidence at protein level;
KW Activator; Nucleus; Phosphoprotein; Reference proteome; Repeat; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..2207
FT /note="Mediator of RNA polymerase II transcription subunit
FT 13-like"
FT /id="PRO_0000076353"
FT REGION 337..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 384..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 431..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 519..574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..767
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 816..847
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1004..1091
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1379..1400
FT /note="Leucine-zipper"
FT REGION 1523..1652
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2042..2077
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 664..668
FT /note="LXXLL motif 1"
FT MOTIF 1224..1228
FT /note="LXXLL motif 2"
FT COMPBIAS 339..358
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 444..461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..479
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..543
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 731..746
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 751..767
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1005..1033
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1055..1091
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1533..1647
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 548
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT MOD_RES 555
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT MOD_RES 812
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT MOD_RES 821
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT MOD_RES 918
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT MOD_RES 2080
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q71F56"
FT CONFLICT 950
FT /note="T -> M (in Ref. 3; BAC65701)"
FT /evidence="ECO:0000305"
FT CONFLICT 1008
FT /note="V -> L (in Ref. 3; BAC65701)"
FT /evidence="ECO:0000305"
FT CONFLICT 1222
FT /note="V -> I (in Ref. 3; BAC65701)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2207 AA; 241758 MW; CC4CE8B90CF30338 CRC64;
MTAAANWVAN GASLEDCHSN LFSLAELTGI KWRRYNFGGH GDCGPIISAP AQDDPILLSF
IRCLQANLLC VWRRDVKPDC KELWIFWWGD EPNLVGVIHH ELQVVEEGLW ENGLSYECRT
LLFKAIHNLL ERCLMDKNFV RIGKWFVRPY DKDEKPVNKS EHLSCAFTFF LHGESNVCTS
VEIAQHQPIY LINEEHLHMA QSSPAPFQVL VSPYGLNGTL TGHAYKMSDP AARKLIEEWH
CFYPMVLRKR EEPREEAELG YDDDFPVAVE VIVGGVRMVY PSAFVLVSQN DIPVPQSGHG
TVAQQGLGSV KDPSNCGMPL TPPTSPEQVV IGESGGVQSA ASHLGSQDGG MSTMHSPKRS
RKTPPKLHSH MVRRVWRECI LSRAQSKRSQ MSTPTREEEA AHSPAAWDFV DPTQRVSCSC
SRHKLLKRCA VGPSRPPAIS QPGFSAGLPS SSSLPPPASS KHKTTERQEK GDKLQKRPLV
PFHHRPSVAE ELCVEQDAPG QKLGLAGIDA SLEVSNTRKY DKQMAVPSRN TSKQMNLNPM
DSPHSPISPL PPTLSPQPRG QEAESLDPPS VPVNPALYGN GLDLQQLSTI EDRTVLVGQR
LPLMAEASET ALYSGLRPSY TESSDRWWQS FRLPSSEDAE FRPPELQGER FDTALDLNPE
STALQRLLAQ PNKRFKIWQD EQPQVQPLPF LDPSPLSQQP GDTLGEVNDP YTFEDGDIKY
IFTANKKCKQ GTEKDSLKKN KSEDGFGTKD VTTPGHSTPV PDGKNAMSIF SSATKTDVRQ
DSAAGRAGSG SLTQVTDLAP SLHDLDNIFD NSDDDELGAV SPALRSSKMP TVGTEERPPG
KDGRAAGPYP PTVADLQRMF PTPPSLEQHP AFSPVMNYKD GVSSETVTAL GMMESPVVSM
VPTHLTEFRM EVEDGLGSPK PEEIKDFSYV HKVPQFQPFV GSSMFAPLKT LPSHCLLPLK
TPDACLFRPS WAVPPKMEQL PMPPAASSIR DGYNNVPSVG SLADPDYVNT PQMNTPVTLN
SAAPASNSGA GVLPSPATPR FSVPTPRTPR TPRTPRGGGT ASGQGSVKYD STDQGSPAST
PSTTRPLNSV EPATMQPIPE AHSLYVTLIL SDSVMNVFKD RNFDSCCICA CNMNIKGADV
GLYIPDSSKE DQYRCTCGFS AIVNRKLGYN SGLFLEDELD IFGKNSDIGQ AAERRLMMCQ
SSGQSTLLPQ VEGARKAPEP PVSLLLLLQN QHTQPFASLS FLDYISSANR HALPCVSWTY
DRVQADNNDY WTECFNALEQ GRQYVDNPTG GKVDEALVRS ATVHCWPHSN VLDTSMLSSQ
DVVRMLLSLQ PFLQDAIQKK RTGRTWENIQ HVQGPLTWQQ FHKMAGRGTY GSEESPEPLP
IPTLLVGYDK EFLTISPFSL PFWERLLLEP YGGHRDVAYI VVCPENEALL EGAKTFFRDL
SAVYEMCRLG QHKPICKVLR DGIMRVGKTV AQKLTEELVS EWFNQPWSSE ESDNHSRLKL
YAQVCRHHLA PYLATLQLDS GLLMPPKHQS PPAEAQGQAT PGNAGSLPSN SGSGAPPAGS
AFNPTSSSSA NPTTSSSSAS SGPPGSSAAS APGITQMNTT SSSGFGGGVG GQNPSAGGSS
TDRTPGNVAC GDTEPGQSCT QSSQDGQDSV TERERIGIPT EPDSADSHAY PPAVVIYMVD
PFTYTAEEDS SSGNFWLLSL MRCYTEMLDH LPEHMRSSFI LQIVPCQYML QTMKDEHVFY
IQYLKSMAFS VYCQCRRPLP TQIHIKSLTG FGPAASIEMT LKNPERPSPI QLYSPPFILA
PIKDKQTEPG ETFGEASQKY NVLFVGYCLS HDQRWLLASC TDLHGELLET CVVNIALPSR
SRKSKVSARK VGLQKLWEWC LGIVQMTSLP WRVVIGRLGR LGHGELKDWS ILLGECSLQT
ISKQLKDVCR MCGISAADSP SILSACLVAM EPQGSFVVMP DAVTMGSVFG RSTALNMQSS
QLNTPQDASC THILVFPTSS TIQVAPANYP NEDGFSPNND DMFVDLPFPD DMDNDIGILM
TGNLHSSPNS SPVPSPGSPS GIGVGSHFQH SRSQGERLLS REAPEELKQQ PLALGYFVST
AKAENLPQWF WSSCPQARNQ CPLFLKASLH HHISVAQTDE LLPARTSQRA PHPLDSKTTS
DVLRFVLEQY NALSWLTCNP ATQDRTSCLP VHFVVLTQLY NAIMNML
