MD15A_ARATH
ID MD15A_ARATH Reviewed; 1335 AA.
AC F4I171; Q56WT7; Q9LMQ6;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 15a;
GN Name=MED15A; Synonyms=MED15_2; OrderedLocusNames=At1g15780;
GN ORFNames=F7H2.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1086-1335.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- INTERACTION:
CC F4I171; Q7XHI5: BHLH133; NbExp=3; IntAct=EBI-21254477, EBI-15195839;
CC F4I171; Q6R0A6: MYB63; NbExp=3; IntAct=EBI-21254477, EBI-15198077;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 15 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF82148.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD94404.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC034256; AAF82148.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE29364.1; -; Genomic_DNA.
DR EMBL; AK221945; BAD94404.1; ALT_INIT; mRNA.
DR PIR; B86292; B86292.
DR RefSeq; NP_173030.1; NM_101446.4.
DR AlphaFoldDB; F4I171; -.
DR SMR; F4I171; -.
DR BioGRID; 23387; 4.
DR IntAct; F4I171; 46.
DR STRING; 3702.AT1G15780.1; -.
DR PaxDb; F4I171; -.
DR PRIDE; F4I171; -.
DR EnsemblPlants; AT1G15780.1; AT1G15780.1; AT1G15780.
DR GeneID; 838147; -.
DR Gramene; AT1G15780.1; AT1G15780.1; AT1G15780.
DR KEGG; ath:AT1G15780; -.
DR Araport; AT1G15780; -.
DR TAIR; locus:2036154; AT1G15780.
DR eggNOG; ENOG502QQV3; Eukaryota.
DR HOGENOM; CLU_003310_0_0_1; -.
DR InParanoid; F4I171; -.
DR PRO; PR:F4I171; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I171; baseline and differential.
DR Genevisible; F4I171; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:TAIR.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:TAIR.
DR GO; GO:0045723; P:positive regulation of fatty acid biosynthetic process; IMP:TAIR.
DR GO; GO:0009751; P:response to salicylic acid; IMP:TAIR.
DR Gene3D; 1.10.246.20; -; 1.
DR InterPro; IPR036529; KIX_dom_sf.
DR InterPro; IPR036546; MED15_KIX.
DR InterPro; IPR044661; MED15a/b/c-like.
DR PANTHER; PTHR33137; PTHR33137; 2.
DR Pfam; PF16987; KIX_2; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..1335
FT /note="Mediator of RNA polymerase II transcription subunit
FT 15a"
FT /id="PRO_0000418348"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 109..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 496..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 567..591
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 683..815
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 947..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1146..1165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 834..882
FT /evidence="ECO:0000255"
FT COMPBIAS 690..815
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 970..986
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1335 AA; 146345 MW; C7B260520E79B5AF CRC64;
MDNNNWRPSL PNGEPAMDTG DWRTQLPPDS RQKIVNKIME TLKKHLPFSG PEGINELRRI
AARFEEKIFS GALNQTDYLR KISMKMLTME TKSQNAAGSS AAIPAANNGT SIDSIPTNQG
QLLPGSLSTN QSQAPQPLLS QTMQNNTASG MTGSTALPSS MPPVSSITNN NTTSVVNQNA
NMQNVAGMLQ DSSGQHGLSS NMFSGPQRQM LGRPHAMSSQ QQQQPYLYQQ QLQQQLLKQN
FQSGNVPNPN SLLPSHIQQQ QQNVLQPNQL HSSQQPGVPT SATQPSTVNS APLQGLHTNQ
QSSPQLSSQQ TTQSMLRQHQ SSMLRQHPQS QQASGIHQQQ SSLPQQSISP LQQQPTQLMR
QQAANSSGIQ QKQMMGQHVV GDMQQQHQQR LLNQQNNVMN IQQQQSQQQP LQQPQQQQKQ
QPPAQQQLMS QQNSLQATHQ NPLGTQSNVA GLQQPQQQML NSQVGNSSLQ NNQHSVHMLS
QPTVGLQRTH QAGHGLYSSQ GQQSQNQPSQ QQMMPQLQSH HQQLGLQQQP NLLQQDVQQR
LQASGQVTGS LLPPQNVVDQ QRQLYQSQRT LPEMPSSSLD STAQTESANG GDWQEEVYQK
IKSMKETYLP DLNEIYQRVA AKLQQDSMPQ QQRSDQLEKL RQFKTMLERM IQFLSVSKSN
IMPALKDKVA YYEKQIIGFL NMHRPRKPVQ QGQLPQSQMQ PMQQPQSQTV QDQSHDNQTN
PQMQSMSMQG AGPRAQQSSM TNMQSNVLSS RPGVSAPQQN IPSSIPASSL ESGQGNTLNN
GQQVAMGSMQ QNTSQLVNNS SASAQSGLST LQSNVNQPQL SSSLLQHQHL KQQQDQQMQL
KQQFQQRQMQ QQQLQARQQQ QQQQLQARQQ AAQLQQMNDM NDLTSRQGMN VSRGMFQQHS
MQGQRANYPL QQLKPGAVSS PQLLQGASPQ MSQHLSPQVD QKNTVNKMGT PLQPANSPFV
VPSPSSTPLA PSPMQVDSEK PGSSSLSMGN IARQQATGMQ GVVQSLAIGT PGISASPLLQ
EFTSPDGNIL NSSTITSGKP SATELPIERL IRAVKSISPQ ALSSAVSDIG SVVSMVDRIA
GSAPGNGSRA SVGEDLVAMT KCRLQARNFM TQEGMMATKK MKRHTTAMPL SVASLGGSVG
DNYKQFAGSE TSDLESTATS DGKKARTETE HALLEEIKEI NQRLIDTVVE ISDDEDAADP
SEVAISSIGC EGTTVRFSFI AVSLSPALKA HLSSTQMSPI QPLRLLVPCS YPNGSPSLLD
KLPVETSKEN EDLSSKAMAR FNILLRSLSQ PMSLKDIAKT WDACARAVIC EYAQQFGGGT
FSSKYGTWEK YVAAS
