MD15C_ARATH
ID MD15C_ARATH Reviewed; 935 AA.
AC Q9SHV7; F4IQE4;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Probable mediator of RNA polymerase II transcription subunit 15c;
GN Name=MED15C; Synonyms=MED15_3; OrderedLocusNames=At2g10440;
GN ORFNames=F12P23.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SHV7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SHV7-2; Sequence=VSP_044029, VSP_044030, VSP_044031,
CC VSP_044032;
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 15 family.
CC {ECO:0000305}.
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DR EMBL; AC007264; AAD28655.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06144.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06145.1; -; Genomic_DNA.
DR PIR; E84491; E84491.
DR RefSeq; NP_001154512.1; NM_001161040.1. [Q9SHV7-2]
DR RefSeq; NP_178842.1; NM_126801.1. [Q9SHV7-1]
DR AlphaFoldDB; Q9SHV7; -.
DR SMR; Q9SHV7; -.
DR STRING; 3702.AT2G10440.1; -.
DR PaxDb; Q9SHV7; -.
DR PRIDE; Q9SHV7; -.
DR EnsemblPlants; AT2G10440.1; AT2G10440.1; AT2G10440. [Q9SHV7-1]
DR EnsemblPlants; AT2G10440.2; AT2G10440.2; AT2G10440. [Q9SHV7-2]
DR GeneID; 815503; -.
DR Gramene; AT2G10440.1; AT2G10440.1; AT2G10440. [Q9SHV7-1]
DR Gramene; AT2G10440.2; AT2G10440.2; AT2G10440. [Q9SHV7-2]
DR KEGG; ath:AT2G10440; -.
DR Araport; AT2G10440; -.
DR TAIR; locus:2039871; AT2G10440.
DR eggNOG; ENOG502QQV3; Eukaryota.
DR HOGENOM; CLU_016518_1_0_1; -.
DR InParanoid; Q9SHV7; -.
DR OMA; NNIACAG; -.
DR OrthoDB; 320308at2759; -.
DR PhylomeDB; Q9SHV7; -.
DR PRO; PR:Q9SHV7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SHV7; baseline and differential.
DR Genevisible; Q9SHV7; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:TAIR.
DR GO; GO:0003713; F:transcription coactivator activity; IEA:InterPro.
DR InterPro; IPR036546; MED15_KIX.
DR InterPro; IPR044661; MED15a/b/c-like.
DR PANTHER; PTHR33137; PTHR33137; 3.
DR Pfam; PF16987; KIX_2; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..935
FT /note="Probable mediator of RNA polymerase II transcription
FT subunit 15c"
FT /id="PRO_0000418350"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 170..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 495..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 548..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 635..654
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 510..526
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 548..602
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 41
FT /note="I -> IVCSLNDYRK (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044029"
FT VAR_SEQ 75
FT /note="K -> KNDYLRKLSETLHYIQRTYTRVLASQVVVNQAQSLPALLPYMQ (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044030"
FT VAR_SEQ 155..286
FT /note="EQKRQEQEQLINQLTNLPTSRPNNRDQQGAFQVSSSQQNNNVTLHAMSQQKN
FT NLQSMTRGQQVGQSQPMMSQQYRQQYPMQQDPQNRNLQKHLDFVQNNTNQFQAASSLRQ
FT TQNITDQQNQPQQLERANPSI -> V (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044031"
FT VAR_SEQ 827..836
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044032"
SQ SEQUENCE 935 AA; 105506 MW; 6E8DACD7174D17F7 CRC64;
MEGNTNWKPN EQGGNRDAAN NRIDWRSQHE PELRQKVLSK IVEKFKEKFH AHEEYKINDI
ASKFEENFYS IATDKTTTSQ QWLQQNTQSN LCVICFAKSG PYNRSRFSKT DTCSVTAAAT
SSAKFVETTY STAFSSIKLT KHSITDQKSV FDTTEQKRQE QEQLINQLTN LPTSRPNNRD
QQGAFQVSSS QQNNNVTLHA MSQQKNNLQS MTRGQQVGQS QPMMSQQYRQ QYPMQQDPQN
RNLQKHLDFV QNNTNQFQAA SSLRQTQNIT DQQNQPQQLE RANPSILIMN IIVASQDSTG
KTVNVNAGNW QEETYQKIKK LKEMCLPVLS LMHQRVAEKL RETESLPPQP MQAQWIEKLK
AGKLSMEHLM FFLNVHRSSV SEKHRDKFSQ YEYHILKFTK SQTMVLRPTQ QQQGQFPPSQ
TAMQTQSPQV HVSQSLYKEQ RRSRLMPSSQ NEASSLLQIR PKLDPRDENI IMASSGNVML
PSVKQNPRAV NTNISSVQSL QKQKRFHHRQ MQQQQPQQGN HQHQMQTNEM NDVRMRERVN
IKARLLEQQV SSSQRQVPKQ ESNVSSSQIQ NHSSPQLVDQ HILPATINKT GTPLNSSGSA
FVAPAPSPVP GDSEMPISVE SPVSGVDEIN STLDSSSKLG TQETPLLFVP PPEPITERPI
DRLIKAFQAA SPKSLAESVS EISSVISMVD MIGGSFPSSG GSRAGLGEDL SERTRNFTTH
EETNLSKRMK RSINIVPPDM SSQIDSYEQL SSLESEVVST TSSGLKVNNI APGYALLQEI
KETNGRLVET VVEICDEDSL GTIVTCTYAP VALSATFKDH YKSGKIIFYV SKCLMQAQIQ
PLRLLFPMDY PYSSPIVLEE ISFDTSVHKY EDLSARTRSR FSLSMKEFSE PGFSKGIAQT
WNDCARATMV EYAERHGGGT FSSKYGAWET VLRAS
