MD19A_ARATH
ID MD19A_ARATH Reviewed; 221 AA.
AC Q9FMP0; Q6NMK7;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 19a;
GN Name=MED19A; Synonyms=MED19_1; OrderedLocusNames=At5g12230;
GN ORFNames=MXC9.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kim C.J., Chen H., Cheuk R., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [6]
RP INTERACTION WITH FIB2.
RX PubMed=30307032; DOI=10.1111/nph.15530;
RA Seo J.S., Diloknawarit P., Park B.S., Chua N.H.;
RT "ELF18-INDUCED LONG NONCODING RNA 1 evicts fibrillarin from mediator
RT subunit to enhance PATHOGENESIS-RELATED GENE 1 (PR1) expression.";
RL New Phytol. 221:2067-2079(2019).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex (PubMed:17560376). Interacts
CC with FIB2 (PubMed:30307032). {ECO:0000269|PubMed:17560376,
CC ECO:0000269|PubMed:30307032}.
CC -!- INTERACTION:
CC Q9FMP0; F4IXJ7: MED6; NbExp=2; IntAct=EBI-1386401, EBI-1386187;
CC Q9FMP0; G3C9P1: RxL44; Xeno; NbExp=2; IntAct=EBI-1386401, EBI-16086576;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the plant Mediator complex subunit 19 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAR20782.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAS47657.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB007727; BAB10041.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91778.1; -; Genomic_DNA.
DR EMBL; BT010725; AAR20782.1; ALT_INIT; mRNA.
DR EMBL; BT011651; AAS47657.1; ALT_INIT; mRNA.
DR RefSeq; NP_196784.1; NM_121261.3.
DR AlphaFoldDB; Q9FMP0; -.
DR BioGRID; 16375; 1.
DR DIP; DIP-39929N; -.
DR IntAct; Q9FMP0; 3.
DR STRING; 3702.AT5G12230.1; -.
DR iPTMnet; Q9FMP0; -.
DR PaxDb; Q9FMP0; -.
DR PRIDE; Q9FMP0; -.
DR ProteomicsDB; 238764; -.
DR EnsemblPlants; AT5G12230.1; AT5G12230.1; AT5G12230.
DR GeneID; 831097; -.
DR Gramene; AT5G12230.1; AT5G12230.1; AT5G12230.
DR KEGG; ath:AT5G12230; -.
DR Araport; AT5G12230; -.
DR TAIR; locus:2177073; AT5G12230.
DR eggNOG; ENOG502R8JR; Eukaryota.
DR HOGENOM; CLU_078385_1_0_1; -.
DR InParanoid; Q9FMP0; -.
DR OMA; SFEVLAW; -.
DR OrthoDB; 1419662at2759; -.
DR PhylomeDB; Q9FMP0; -.
DR PRO; PR:Q9FMP0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMP0; baseline and differential.
DR Genevisible; Q9FMP0; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR019403; Mediator_Med19_met.
DR PANTHER; PTHR22536; PTHR22536; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..221
FT /note="Mediator of RNA polymerase II transcription subunit
FT 19a"
FT /id="PRO_0000419191"
FT REGION 101..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 155..178
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 186..201
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 221 AA; 25697 MW; 177F2531AD9E599B CRC64;
MEPERLKFGG PRELCGAADL ISQFKLVQHH EFFCKKSLPV SLSDSHYLHN VVGDTEIRKG
EGMQLDQLIE SISQSRETNI RIQPFDIDEL QESFQLNDMT PVELPPAEKG APTIPSKSKS
ESKDRDRKHK KHKDRDKDKD REHKKHKHKH KDRSKDKDKD KDRDRKKDKN GHHDSGDHSK
KHHDKKRKHD GDEDLNDVQR HKKNKHKSSK LDEVGAIRVA G
