MD20A_ARATH
ID MD20A_ARATH Reviewed; 219 AA.
AC Q6NPF4; Q9SL32;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 20a;
GN Name=MED20A; Synonyms=MED20_1; OrderedLocusNames=At2g28230;
GN ORFNames=T3B23.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [5]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- INTERACTION:
CC Q6NPF4; Q9SJZ6: MED18; NbExp=3; IntAct=EBI-1386270, EBI-1386265;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 20 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD29830.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC006202; AAD29830.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC08093.1; -; Genomic_DNA.
DR EMBL; BT010690; AAR20747.1; -; mRNA.
DR EMBL; BT010970; AAR24748.1; -; mRNA.
DR PIR; D84682; D84682.
DR RefSeq; NP_001324170.1; NM_001336151.1.
DR RefSeq; NP_180390.2; NM_128383.4.
DR AlphaFoldDB; Q6NPF4; -.
DR SMR; Q6NPF4; -.
DR IntAct; Q6NPF4; 2.
DR STRING; 3702.AT2G28230.1; -.
DR PaxDb; Q6NPF4; -.
DR PRIDE; Q6NPF4; -.
DR ProteomicsDB; 238325; -.
DR DNASU; 817369; -.
DR EnsemblPlants; AT2G28230.1; AT2G28230.1; AT2G28230.
DR GeneID; 817369; -.
DR Gramene; AT2G28230.1; AT2G28230.1; AT2G28230.
DR KEGG; ath:AT2G28230; -.
DR Araport; AT2G28230; -.
DR TAIR; locus:2062799; AT2G28230.
DR eggNOG; KOG1383; Eukaryota.
DR HOGENOM; CLU_080552_0_0_1; -.
DR InParanoid; Q6NPF4; -.
DR OMA; VKWLMHW; -.
DR OrthoDB; 1112080at2759; -.
DR PhylomeDB; Q6NPF4; -.
DR PRO; PR:Q6NPF4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6NPF4; baseline and differential.
DR Genevisible; Q6NPF4; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0035196; P:miRNA processing; IMP:TAIR.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:TAIR.
DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:TAIR.
DR GO; GO:2000031; P:regulation of salicylic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR013921; Mediator_Med20.
DR PANTHER; PTHR12465; PTHR12465; 1.
DR Pfam; PF08612; Med20; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..219
FT /note="Mediator of RNA polymerase II transcription subunit
FT 20a"
FT /id="PRO_0000418119"
SQ SEQUENCE 219 AA; 25127 MW; C3AC5D1BBA3936EC CRC64;
MPVKWVLHWQ PNQGSTVSSQ ILNEATQCVE SINGVKEGRW KATLNYYKPM LKDQANQLEF
PRDFLGISLA DQPNKYYFII RTQRIVLEAD SSIQLIMEKL QSYKSKVALY FDGFQYQLGD
FRLRVGKVVP THSENVRGIV MEVEYLPISS MEKAQKVMEE FLEIWNEALA KRSLPGKFVN
IDLNFGEFGL GDIYTPQHTA VRYALVMAHM IATVQAVRG
