ID   MD20B_ARATH             Reviewed;         219 AA.
AC   Q9M0S0;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 20b;
GN   Name=MED20B; Synonyms=MED20_2; OrderedLocusNames=At4g09070;
GN   ORFNames=F23J3.100;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   IDENTIFICATION, AND NOMENCLATURE.
RX   PubMed=22021418; DOI=10.1104/pp.111.188300;
RA   Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT   "The Mediator complex in plants: structure, phylogeny, and expression
RT   profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT   (rice) during reproduction and abiotic stress.";
RL   Plant Physiol. 157:1609-1627(2011).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. The Mediator complex, having a compact
CC       conformation in its free form, is recruited to promoters by direct
CC       interactions with regulatory proteins and serves for the assembly of a
CC       functional preinitiation complex with RNA polymerase II and the general
CC       transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 20 family.
CC       {ECO:0000305}.
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DR   EMBL; AL161514; CAB78031.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE82719.1; -; Genomic_DNA.
DR   PIR; G85091; G85091.
DR   RefSeq; NP_192646.1; NM_116976.1.
DR   AlphaFoldDB; Q9M0S0; -.
DR   SMR; Q9M0S0; -.
DR   STRING; 3702.AT4G09070.1; -.
DR   PaxDb; Q9M0S0; -.
DR   PRIDE; Q9M0S0; -.
DR   EnsemblPlants; AT4G09070.1; AT4G09070.1; AT4G09070.
DR   GeneID; 826485; -.
DR   Gramene; AT4G09070.1; AT4G09070.1; AT4G09070.
DR   KEGG; ath:AT4G09070; -.
DR   Araport; AT4G09070; -.
DR   TAIR; locus:2122318; AT4G09070.
DR   eggNOG; KOG1383; Eukaryota.
DR   HOGENOM; CLU_080552_0_0_1; -.
DR   InParanoid; Q9M0S0; -.
DR   OMA; CEMEYLP; -.
DR   OrthoDB; 1112080at2759; -.
DR   PhylomeDB; Q9M0S0; -.
DR   PRO; PR:Q9M0S0; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; Q9M0S0; baseline.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR013921; Mediator_Med20.
DR   PANTHER; PTHR12465; PTHR12465; 1.
DR   Pfam; PF08612; Med20; 1.
PE   3: Inferred from homology;
KW   Nucleus; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..219
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   20b"
FT                   /id="PRO_0000418120"
SQ   SEQUENCE   219 AA;  25123 MW;  C4F59854A1649DBF CRC64;
     MPVKWLLHWQ PNQGSTFSSQ ILNEVTQSIE SLNGVKEGTW KATLNYYKPM LQDQANQAEF
     PREFVGISLP EEPDKYYFVI RSQRIVVEAD SSIQMIMESL QSYKCKLSFY FEGLEYQLGD
     FRLRVGKVVP THAETIRGVV MEVEYLPISS MGMAKKLMEE FLEIWQEAMS KRSLPGKFVN
     KELNFEKFGL GDNYTPQHTA VGYAFFMANL MAAIQAGRG