MD26A_ARATH
ID MD26A_ARATH Reviewed; 580 AA.
AC F4J4Y5; F4J4Y6; Q9SG88;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 66.
DE RecName: Full=Probable mediator of RNA polymerase II transcription subunit 26a;
GN Name=MED26A; Synonyms=MED26_1; OrderedLocusNames=At3g10820;
GN ORFNames=T7M13.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors (By similarity). May play a role in transcription
CC elongation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the Mediator complex. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4J4Y5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4J4Y5-2; Sequence=VSP_044034, VSP_044035;
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 26 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF19567.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC011708; AAF19567.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74960.1; -; Genomic_DNA.
DR RefSeq; NP_001189856.1; NM_001202927.1. [F4J4Y5-1]
DR AlphaFoldDB; F4J4Y5; -.
DR iPTMnet; F4J4Y5; -.
DR PaxDb; F4J4Y5; -.
DR PRIDE; F4J4Y5; -.
DR EnsemblPlants; AT3G10820.2; AT3G10820.2; AT3G10820. [F4J4Y5-1]
DR GeneID; 820251; -.
DR Gramene; AT3G10820.2; AT3G10820.2; AT3G10820. [F4J4Y5-1]
DR KEGG; ath:AT3G10820; -.
DR Araport; AT3G10820; -.
DR TAIR; locus:2103167; AT3G10820.
DR eggNOG; ENOG502QR7F; Eukaryota.
DR HOGENOM; CLU_024836_0_0_1; -.
DR InParanoid; F4J4Y5; -.
DR OrthoDB; 813723at2759; -.
DR PRO; PR:F4J4Y5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J4Y5; baseline and differential.
DR Genevisible; F4J4Y5; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR Gene3D; 1.20.930.10; -; 1.
DR InterPro; IPR044805; MD26A/B-like.
DR InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR InterPro; IPR017923; TFIIS_N.
DR PANTHER; PTHR46554; PTHR46554; 1.
DR Pfam; PF08711; Med26; 1.
DR SMART; SM00509; TFS2N; 1.
DR SUPFAM; SSF47676; SSF47676; 1.
DR PROSITE; PS51319; TFIIS_N; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Coiled coil; Elongation factor; Nucleus;
KW Protein biosynthesis; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..580
FT /note="Probable mediator of RNA polymerase II transcription
FT subunit 26a"
FT /id="PRO_0000418353"
FT DOMAIN 108..183
FT /note="TFIIS N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT REGION 244..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 347..368
FT /evidence="ECO:0000255"
FT COMPBIAS 244..259
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 278..307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 322..337
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 233..288
FT /note="ILDCLDCDGNPRHSVESKHERKSQSSAGRRPKGTNDANVVGRYCNDQQTRRE
FT EADV -> IVWTAMEILVTVWSPNMKENRKVVRGGDPREQMMQMWLGGTAMINRRGEKK
FT LMLDL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044034"
FT VAR_SEQ 289..580
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_044035"
SQ SEQUENCE 580 AA; 65872 MW; CA3E285CC1456A7F CRC64;
MAKMRPSVSL DTWREYFRRG DSDIFGIIDH AIMVAAADWP KEFKSRSDRI AELLFSCKVS
RCIGCDHLEL SIAGDEAAVE IVGVGGGGDR GDSGVATGEG EEASVSVDEV MRIRDILSNK
DDEKDSVLLE SLRKLESMSM SVDILKDTEI GKAVNGLRRH SSDKISKLAK TLFAEWKRLV
DQWMNTPEEM AGTEGTPESL NLSVIDEEEA FPSPPHDLDI YAPEPNGFEL SQILDCLDCD
GNPRHSVESK HERKSQSSAG RRPKGTNDAN VVGRYCNDQQ TRREEADVRP MKHSATDVVE
PKRQTKQSRE QMVSAIQRKP TAVTEQKRKL AGPQQDKLKA LDPDSKFEFA KRKLQESYHQ
HENAKRQRTI QVLETIPKQN KVQKPQLKRP ATRRYIYWYF WSSEFPGYEL PSLILNLANP
RDSSSQFSRH DELMIDIPVY FGLVLVQIDK AEMVDEMMIF KKMSVLLTEK DFVDAIVSVS
LKDLTRSLFV RFEAKACPKF DLIHKTSVET VRCVGKTFMH MLSKVSREFP PRNRTHPGGL
LYQRFDPTHL SLARQQVAIC KETDSQYPHE IVIHWTSASA
