ID   MD26B_ARATH             Reviewed;         436 AA.
AC   Q9FHK9; Q8W477;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Probable mediator of RNA polymerase II transcription subunit 26b;
GN   Name=MED26B; Synonyms=MED26_2; OrderedLocusNames=At5g05140;
GN   ORFNames=K2A11.1;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA   Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT   features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT   clones.";
RL   DNA Res. 6:183-195(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 137-436.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION, AND NOMENCLATURE.
RX   PubMed=22021418; DOI=10.1104/pp.111.188300;
RA   Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT   "The Mediator complex in plants: structure, phylogeny, and expression
RT   profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT   (rice) during reproduction and abiotic stress.";
RL   Plant Physiol. 157:1609-1627(2011).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. The Mediator complex, having a compact
CC       conformation in its free form, is recruited to promoters by direct
CC       interactions with regulatory proteins and serves for the assembly of a
CC       functional preinitiation complex with RNA polymerase II and the general
CC       transcription factors (By similarity). May play a role in transcription
CC       elongation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 26 family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAL32865.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AB018111; BAB09690.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90833.1; -; Genomic_DNA.
DR   EMBL; AY062787; AAL32865.1; ALT_INIT; mRNA.
DR   EMBL; AY081599; AAM10161.1; -; mRNA.
DR   RefSeq; NP_196133.3; NM_120596.4.
DR   AlphaFoldDB; Q9FHK9; -.
DR   SMR; Q9FHK9; -.
DR   STRING; 3702.AT5G05140.1; -.
DR   iPTMnet; Q9FHK9; -.
DR   PaxDb; Q9FHK9; -.
DR   PRIDE; Q9FHK9; -.
DR   ProteomicsDB; 238327; -.
DR   EnsemblPlants; AT5G05140.1; AT5G05140.1; AT5G05140.
DR   GeneID; 830396; -.
DR   Gramene; AT5G05140.1; AT5G05140.1; AT5G05140.
DR   KEGG; ath:AT5G05140; -.
DR   Araport; AT5G05140; -.
DR   TAIR; locus:2156814; AT5G05140.
DR   eggNOG; ENOG502QR7F; Eukaryota.
DR   HOGENOM; CLU_024836_2_0_1; -.
DR   InParanoid; Q9FHK9; -.
DR   OMA; ICEILFT; -.
DR   OrthoDB; 813723at2759; -.
DR   PhylomeDB; Q9FHK9; -.
DR   PRO; PR:Q9FHK9; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FHK9; baseline and differential.
DR   Genevisible; Q9FHK9; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.930.10; -; 1.
DR   InterPro; IPR044805; MD26A/B-like.
DR   InterPro; IPR003617; TFIIS/CRSP70_N_sub.
DR   InterPro; IPR035441; TFIIS/LEDGF_dom_sf.
DR   InterPro; IPR017923; TFIIS_N.
DR   PANTHER; PTHR46554; PTHR46554; 1.
DR   Pfam; PF08711; Med26; 1.
DR   SMART; SM00509; TFS2N; 1.
DR   SUPFAM; SSF47676; SSF47676; 1.
DR   PROSITE; PS51319; TFIIS_N; 1.
PE   2: Evidence at transcript level;
KW   Coiled coil; Elongation factor; Nucleus; Protein biosynthesis;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..436
FT                   /note="Probable mediator of RNA polymerase II transcription
FT                   subunit 26b"
FT                   /id="PRO_0000418354"
FT   DOMAIN          139..214
FT                   /note="TFIIS N-terminal"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00649"
FT   REGION          71..111
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          263..376
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          408..436
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          382..402
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        272..295
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        326..376
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        420..436
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   436 AA;  49135 MW;  96AE3DFBAB51994E CRC64;
     MKASGSLDSW REYFRRRGDS DIFGIIDHAI MVAATDCPNK FKSRRDKIAE LLFSCRVNRC
     VGCDHLELSV PGDDEANRGT TGNGGGGTAV DEDYEVAGGS KESKANSSRG DNNQIVSNYT
     FDEAEALSDE IEEFSVVSKE VARIKEILLN KEDEPNSVLL DSLRHLKLMS LNVDILKSTE
     IGKAVNGLRK HSSDKIRQLA KTLIAEWKEL VDQWVNTTKE ITGAEGTPES ANPSVLDEEE
     AFPSLPYDVD IFTPEPNGFE ISHFFDSLDF DGNPRNSEEH NTSREHERRP QNIAKRKPEG
     TQMRIQDAPF RSIKPSSATD FDGTRRPVKQ STEQRMKNET VSVHKSEKPM IQRKPVVTEQ
     KRKAPGPQQE KLKGLDADAK FEFAKRKLQE SYQHHENAKK QRTIQVLEMI PKQGSAQKPQ
     LKRPGMSNRN WANGRK