ID   MD27A_XENLA             Reviewed;         311 AA.
AC   Q642Q3;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 45.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 27-A;
DE   AltName: Full=Cofactor required for Sp1 transcriptional activation subunit 8-A;
DE            Short=CRSP complex subunit 8-A;
DE   AltName: Full=Mediator complex subunit 27-A;
GN   Name=med27-a; Synonyms=crsp8-a;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Oocyte;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 27 family.
CC       {ECO:0000305}.
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DR   EMBL; BC081194; AAH81194.1; -; mRNA.
DR   RefSeq; NP_001087771.1; NM_001094302.1.
DR   AlphaFoldDB; Q642Q3; -.
DR   SMR; Q642Q3; -.
DR   DNASU; 447595; -.
DR   GeneID; 447595; -.
DR   KEGG; xla:447595; -.
DR   CTD; 447595; -.
DR   Xenbase; XB-GENE-6054390; med27.L.
DR   OrthoDB; 1256412at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 447595; Expressed in gastrula and 19 other tissues.
DR   GO; GO:0016592; C:mediator complex; IEA:InterPro.
DR   InterPro; IPR021627; Mediator_Med27.
DR   PANTHER; PTHR13130; PTHR13130; 1.
DR   Pfam; PF11571; Med27; 1.
PE   2: Evidence at transcript level;
KW   Activator; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..311
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   27-A"
FT                   /id="PRO_0000305013"
SQ   SEQUENCE   311 AA;  35523 MW;  36FD6D70F7AE41CE CRC64;
     MADTLNVGVN LEAFSQAIHC IQALRSSVTR VFDCLKDGMK NKESQEVRER AFVSEFQDNL
     HCVNRDLNEL ERLSNLVGKP SENHPLHNSG LLSLDPVHDK TPLYSQLLQA YKWSNKLQFH
     AGLASGLLNQ QSLKRSANQM GVSAKRRPKV QPTTLALPPQ YIDDVISRID RMFPEMTIQL
     SRPNGSSAML LVTLGKVLKV VVVMRSLFID RTIVKGYNEN VYTEDGKLDI WSKSNYQVFQ
     KVTDHATTAL LHYQLPQMPD VVVRSFMTWL RSYIKLFQAP CQRCGKFLQD GLPPTWRDFR
     TLEAFHDSCR Q