MD2BP_MOUSE
ID MD2BP_MOUSE Reviewed; 276 AA.
AC Q9DCX1; Q99J05; Q9D0Y3;
DT 26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=MAD2L1-binding protein;
GN Name=Mad2l1bp; Synonyms=Mad2lbp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May function to silence the spindle checkpoint and allow
CC mitosis to proceed through anaphase by binding MAD2L1 after it has
CC become dissociated from the MAD2L1-CDC20 complex. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with MAD2L1. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000250}. Note=During early mitosis,
CC unevenly distributed throughout the nucleoplasm. From metaphase to
CC anaphase, concentrated on the spindle (By similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MAD2L1BP family. {ECO:0000305}.
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DR EMBL; AK002377; BAB22054.1; -; mRNA.
DR EMBL; AK004224; BAB23226.1; -; mRNA.
DR EMBL; BC005768; AAH05768.1; -; mRNA.
DR CCDS; CCDS28821.1; -.
DR RefSeq; NP_079925.2; NM_025649.3.
DR AlphaFoldDB; Q9DCX1; -.
DR SMR; Q9DCX1; -.
DR BioGRID; 211577; 30.
DR IntAct; Q9DCX1; 32.
DR MINT; Q9DCX1; -.
DR STRING; 10090.ENSMUSP00000125915; -.
DR PhosphoSitePlus; Q9DCX1; -.
DR EPD; Q9DCX1; -.
DR MaxQB; Q9DCX1; -.
DR PaxDb; Q9DCX1; -.
DR PeptideAtlas; Q9DCX1; -.
DR PRIDE; Q9DCX1; -.
DR ProteomicsDB; 252757; -.
DR Antibodypedia; 30554; 311 antibodies from 31 providers.
DR DNASU; 66591; -.
DR Ensembl; ENSMUST00000171172; ENSMUSP00000125915; ENSMUSG00000034509.
DR GeneID; 66591; -.
DR KEGG; mmu:66591; -.
DR UCSC; uc008cru.2; mouse.
DR CTD; 9587; -.
DR MGI; MGI:1913841; Mad2l1bp.
DR VEuPathDB; HostDB:ENSMUSG00000034509; -.
DR eggNOG; ENOG502QTUF; Eukaryota.
DR GeneTree; ENSGT00390000003812; -.
DR HOGENOM; CLU_079654_0_0_1; -.
DR InParanoid; Q9DCX1; -.
DR OMA; ATAWEDY; -.
DR OrthoDB; 1492026at2759; -.
DR PhylomeDB; Q9DCX1; -.
DR TreeFam; TF331730; -.
DR BioGRID-ORCS; 66591; 11 hits in 75 CRISPR screens.
DR ChiTaRS; Mad2l1bp; mouse.
DR PRO; PR:Q9DCX1; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9DCX1; protein.
DR Bgee; ENSMUSG00000034509; Expressed in ileal epithelium and 225 other tissues.
DR Genevisible; Q9DCX1; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005819; C:spindle; IEA:UniProtKB-SubCell.
DR GO; GO:1902426; P:deactivation of mitotic spindle assembly checkpoint; ISO:MGI.
DR GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISO:MGI.
DR GO; GO:0007096; P:regulation of exit from mitosis; ISO:MGI.
DR InterPro; IPR009511; MAD1/Cdc20-bound-Mad2-bd.
DR PANTHER; PTHR15681; PTHR15681; 1.
DR Pfam; PF06581; p31comet; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Nucleus; Reference proteome.
FT CHAIN 1..276
FT /note="MAD2L1-binding protein"
FT /id="PRO_0000096311"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 49..81
FT /note="Interaction with MAD2L1"
FT /evidence="ECO:0000250"
FT CONFLICT 51..52
FT /note="EP -> DA (in Ref. 1; BAB22054)"
FT /evidence="ECO:0000305"
FT CONFLICT 104
FT /note="V -> A (in Ref. 2; AAH05768)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 276 AA; 31140 MW; 27C05DEBFD78D20D CRC64;
MAASGEEDMS ELSPAAAPNL DWYEKPEETH APEVDLETVI PPAQEPSNPA EPFCPRDLVP
VVFPGPVSQE DCCQFTCELL KHILYQRHQL PLPYEQLKHF YRKVPQAEDT ARKKAWLATE
ARNRKCQQAL AELESVLSHL RDFFARTLVP QVLILLGGNA LSPKEFYELD LSRLAPFGVD
QGLNTAACLR RLFRAIFLAD PFSELQTPPL MGTIVMVQGH RDCGEDWFQP KLNYRVPSRG
HKLTVTLSCG RPSVPAMASE DYIWFQAPVT LKGFHE
