ID   MD2L1_DICDI             Reviewed;         203 AA.
AC   Q556Y9; Q86JT2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Mitotic spindle assembly checkpoint protein MAD2A;
DE   AltName: Full=Mitotic arrest deficient 2-like protein 1;
DE            Short=MAD2-like protein 1;
GN   Name=mad2l1-1; Synonyms=mad2a-1; ORFNames=DDB_G0273201;
GN   and
GN   Name=mad2l1-2; Synonyms=mad2a-2; ORFNames=DDB_G0273727;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the spindle-assembly checkpoint that prevents
CC       the onset of anaphase until all chromosomes are properly aligned at the
CC       metaphase plate. Required for the execution of the mitotic checkpoint
CC       which monitors the process of kinetochore-spindle attachment and
CC       inhibits the activity of the anaphase promoting complex until all
CC       chromosomes are aligned at the metaphase plate (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with cdc20. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere,
CC       kinetochore {ECO:0000250}. Cytoplasm {ECO:0000250}.
CC   -!- DOMAIN: The protein has two highly different native conformations, an
CC       inactive open conformation that cannot bind cdc20 and that predominates
CC       in cytosolic monomers, and an active closed conformation.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MAD2 family. {ECO:0000305}.
CC   -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC       AX4. These strains contain a duplication of a segment of 750 kb of
CC       chromosome 2 compared to the corresponding sequence in strain AX2.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000011; EAL70554.1; -; Genomic_DNA.
DR   EMBL; AAFI02000009; EAL70818.1; -; Genomic_DNA.
DR   RefSeq; XP_644480.1; XM_639388.1.
DR   RefSeq; XP_644770.1; XM_639678.1.
DR   AlphaFoldDB; Q556Y9; -.
DR   SMR; Q556Y9; -.
DR   STRING; 44689.DDB0232272; -.
DR   PaxDb; Q556Y9; -.
DR   EnsemblProtists; EAL70554; EAL70554; DDB_G0273727.
DR   EnsemblProtists; EAL70818; EAL70818; DDB_G0273201.
DR   GeneID; 8618872; -.
DR   GeneID; 8619104; -.
DR   KEGG; ddi:DDB_G0273201; -.
DR   KEGG; ddi:DDB_G0273727; -.
DR   dictyBase; DDB_G0273201; -.
DR   dictyBase; DDB_G0273727; -.
DR   eggNOG; KOG3285; Eukaryota.
DR   HOGENOM; CLU_072097_0_0_1; -.
DR   InParanoid; Q556Y9; -.
DR   OMA; MLDGNCT; -.
DR   PhylomeDB; Q556Y9; -.
DR   Reactome; R-DDI-141405; Inhibition of the proteolytic activity of APC/C required for the onset of anaphase by mitotic spindle checkpoint components.
DR   Reactome; R-DDI-141430; Inactivation of APC/C via direct inhibition of the APC/C complex.
DR   Reactome; R-DDI-174184; Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
DR   Reactome; R-DDI-176409; APC/C:Cdc20 mediated degradation of mitotic proteins.
DR   Reactome; R-DDI-179409; APC-Cdc20 mediated degradation of Nek2A.
DR   PRO; PR:Q556Y9; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0000776; C:kinetochore; ISS:dictyBase.
DR   GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0099139; P:cheating during chimeric sorocarp development; IMP:dictyBase.
DR   GO; GO:0007094; P:mitotic spindle assembly checkpoint signaling; ISS:dictyBase.
DR   Gene3D; 3.30.900.10; -; 1.
DR   InterPro; IPR003511; HORMA_dom.
DR   InterPro; IPR036570; HORMA_dom_sf.
DR   InterPro; IPR027097; Mad2.
DR   InterPro; IPR045091; Mad2-like.
DR   PANTHER; PTHR11842; PTHR11842; 1.
DR   PANTHER; PTHR11842:SF11; PTHR11842:SF11; 1.
DR   Pfam; PF02301; HORMA; 1.
DR   SUPFAM; SSF56019; SSF56019; 1.
DR   PROSITE; PS50815; HORMA; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Cytoplasm; Kinetochore;
KW   Mitosis; Nucleus; Reference proteome.
FT   CHAIN           1..203
FT                   /note="Mitotic spindle assembly checkpoint protein MAD2A"
FT                   /id="PRO_0000328297"
FT   DOMAIN          14..196
FT                   /note="HORMA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00109"
FT   REGION          194..203
FT                   /note="Required for assuming the closed conformation and
FT                   for interaction with cdc20"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   203 AA;  23050 MW;  F2E3A38F64EE9273 CRC64;
     MQAAVASKTN ISLKGSTEIV TEFFSYSINT ILFQRGLYPP ESFTRVAKYG LPILVTNDQS
     LKDYLDNVLK QLSEWLLSGD VQKLVLVITD IVTKEVLERW VFDVTTDIPK EGEAPRQKPE
     KEIMNEIQAI IRQITASVTF LPLLPNACTF DLLVYTSKDL AVPQKWEESD PKFITNSQQV
     KLRSFTTTIH KVESMVAYKI SND