MD33A_ARATH
ID MD33A_ARATH Reviewed; 1309 AA.
AC Q9LUG9; Q0WM25; Q8GZA2;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 33A;
DE AltName: Full=REF4-related 1 protein;
DE AltName: Full=REF4-resembling 1 protein;
GN Name=MED33A; Synonyms=MED24A, MED5_2, MED5A, RFR1;
GN OrderedLocusNames=At3g23590; ORFNames=MDB19.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Seki M., Iida K., Satou M., Sakurai T., Akiyama K., Ishida J., Nakajima M.,
RA Enju A., Kamiya A., Narusaka M., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Arabidopsis thaliana full-length cDNA.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 940-1309.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR
RP COMPLEX, AND NOMENCLATURE.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP IDENTIFICATION.
RX PubMed=18430946; DOI=10.1534/genetics.107.083881;
RA Stout J., Romero-Severson E., Ruegger M.O., Chapple C.;
RT "Semidominant mutations in reduced epidermal fluorescence 4 reduce
RT phenylpropanoid content in Arabidopsis.";
RL Genetics 178:2237-2251(2008).
RN [7]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=22021418; DOI=10.1104/pp.111.188300;
RA Mathur S., Vyas S., Kapoor S., Tyagi A.K.;
RT "The Mediator complex in plants: structure, phylogeny, and expression
RT profiling of representative genes in a dicot (Arabidopsis) and a monocot
RT (rice) during reproduction and abiotic stress.";
RL Plant Physiol. 157:1609-1627(2011).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22167189; DOI=10.1074/jbc.m111.312298;
RA Bonawitz N.D., Soltau W.L., Blatchley M.R., Powers B.L., Hurlock A.K.,
RA Seals L.A., Weng J.K., Stout J., Chapple C.;
RT "REF4 and RFR1, subunits of the transcriptional coregulatory complex
RT mediator, are required for phenylpropanoid homeostasis in Arabidopsis.";
RL J. Biol. Chem. 287:5434-5445(2012).
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. The Mediator complex, having a compact
CC conformation in its free form, is recruited to promoters by direct
CC interactions with regulatory proteins and serves for the assembly of a
CC functional preinitiation complex with RNA polymerase II and the general
CC transcription factors. Involved in the repression of phenylpropanoid
CC biosynthesis. May compete with MED33B for common binding partners or
CC for occupancy in Mediator. {ECO:0000269|PubMed:22167189}.
CC -!- SUBUNIT: Component of the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, but higher levels of
CC sinapate esters in leaves. {ECO:0000269|PubMed:22167189}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 33 family.
CC {ECO:0000305}.
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DR EMBL; AB023036; BAB02777.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76780.1; -; Genomic_DNA.
DR EMBL; AK117119; BAC41797.1; -; mRNA.
DR EMBL; AK230008; BAF01832.1; -; mRNA.
DR RefSeq; NP_189001.1; NM_113262.4.
DR AlphaFoldDB; Q9LUG9; -.
DR IntAct; Q9LUG9; 1.
DR STRING; 3702.AT3G23590.1; -.
DR PaxDb; Q9LUG9; -.
DR PRIDE; Q9LUG9; -.
DR ProteomicsDB; 238246; -.
DR EnsemblPlants; AT3G23590.1; AT3G23590.1; AT3G23590.
DR GeneID; 821938; -.
DR Gramene; AT3G23590.1; AT3G23590.1; AT3G23590.
DR KEGG; ath:AT3G23590; -.
DR Araport; AT3G23590; -.
DR TAIR; locus:2088050; AT3G23590.
DR eggNOG; ENOG502QRBB; Eukaryota.
DR HOGENOM; CLU_003077_0_0_1; -.
DR InParanoid; Q9LUG9; -.
DR OMA; LPNEVPC; -.
DR OrthoDB; 87466at2759; -.
DR PhylomeDB; Q9LUG9; -.
DR PRO; PR:Q9LUG9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUG9; baseline and differential.
DR Genevisible; Q9LUG9; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:2000762; P:regulation of phenylpropanoid metabolic process; IMP:TAIR.
DR InterPro; IPR039638; MED33A/B.
DR PANTHER; PTHR33739; PTHR33739; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phenylpropanoid metabolism; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..1309
FT /note="Mediator of RNA polymerase II transcription subunit
FT 33A"
FT /id="PRO_0000418344"
FT REGION 809..829
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 809..828
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 127
FT /note="Y -> H (in Ref. 3; BAC41797)"
FT /evidence="ECO:0000305"
FT CONFLICT 1154..1158
FT /note="HVLIK -> RVLIR (in Ref. 4; BAF01832)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1309 AA; 143094 MW; 4E85EBC186409D89 CRC64;
MVVPGRRTVW DCVIELTKMA QENCVDPRLW ASQLSSNLKF FAVELPSTEL AEVIVSYICW
DNNVPIVWKF LERAMALKLV SPLVVLALLA DRVVPTRSTQ QAAYRIYLEL LKRNMFTIKD
HISGPHYQKV MISVSNILRL SELFDLDTSK PGVLLVEFVF KMVSQLLDAA LSDEGLLELS
QDSSSQWLVK SQDMEIDAPE RYNEKTGSLE KLQSLNTIMA IELIAEFLRN TVIARLLYLV
SSNRASKWHE FVQKVQLLGE NSSALKHSKV LNSGDLLQLI SNRRFGYSYD SKVTSARKSN
AIVDFGSLSS YAGLCHGASL SSLWLPLDLV FEDAMDGYQV NPTSAIEIIT GLAKTLKEIN
GSTWHDTFLG LWIAALRLVQ RERDPIEGPI PRLDTRLCMS LCIVPLVVAN LIEEGKYESV
MEKLRDDLVT SLQVLGDFPG LLAPPKCVVS AANKAATKAI LFLSGGNVGK SCFDVINMKD
MPVNCSGNMR HLIVEACIAR NILDMSAYSW PGYVNGRINQ IPQSLPNEVP CWSSFVKGAP
LNAAMVNTLV SVPASSLAEL EKLFEVAVKG SDDEKISAAT VLCGASLTRG WNIQEHTVEY
LTRLLSPPVP ADYSRAENHL IGYACMLNVV IVGIGSVDSI QIFSLHGMVP QLACSLMPIC
EEFGSYTPSV SWTLPSGEAI SAYSVFSNAF TLLLKLWRFN HPPIEHGVGD VPTVGSQLTP
EHLLSVRNSY LVSSEILDRD RNRKRLSEVA RAASCQPVFV DSFPKLKVWY RQHQRCIAAT
LSGLTHGSPV HQTVEALLNM TFGKVRGSQT LNPVNSGTSS SSGAASEDSN IRPEFPAWDI
LKAVPYVVDA ALTACTHGRL SPRQLATGLK DLADFLPASL ATIVSYFSAE VSRGVWKPVF
MNGVDWPSPA TNLSTVEEYI TKILATTGVD IPSLAPGGSS PATLPLPLAA FVSLTITYKI
DKASERFLNL AGPALECLAA GCPWPCMPIV ASLWTQKAKR WFDFLVFSAS RTVFLHNQDA
VIQLLRNCFS ATLGLNAAPM SNDGGVGALL GHGFGSHFYG GISPVAPGIL YLRMYRALRD
TVSVSEEILS LLIHSVEDIA QNRLSKEKLE KLKTVKNGSR YGQSSLATAM TQVKLAASLS
ASLVWLTGGL GVVHVLIKET IPSWFLSTDK SDREQGPSDL VAELRGHALA YFVVLCGALT
WGVDSRSSAS KRRRQAILGS HLEFIASALD GKISVGCETA TWRTYISGLV SLMVSCLPLW
VTEIDTEVLK SLSNGLRKWG KDELAIVLLS LGGLKTMDYA ADFIIHLRS
