MDA2_ARATH
ID MDA2_ARATH Reviewed; 211 AA.
AC F4KF27; Q9FLF2;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Mediator-associated protein 2;
GN OrderedLocusNames=At5g64680; ORFNames=MUB3.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=17560376; DOI=10.1016/j.molcel.2007.05.007;
RA Baeckstroem S., Elfving N., Nilsson R., Wingsle G., Bjoerklund S.;
RT "Purification of a plant mediator from Arabidopsis thaliana identifies PFT1
RT as the Med25 subunit.";
RL Mol. Cell 26:717-729(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-173, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- SUBUNIT: Associated with the Mediator complex.
CC {ECO:0000269|PubMed:17560376}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4KF27-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4KF27-2; Sequence=VSP_044126;
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DR EMBL; AB010076; BAB11435.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97936.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97937.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97938.1; -; Genomic_DNA.
DR EMBL; AK118987; BAC43563.1; -; mRNA.
DR EMBL; BT005670; AAO64090.1; -; mRNA.
DR RefSeq; NP_001154796.1; NM_001161324.1. [F4KF27-1]
DR RefSeq; NP_201273.1; NM_125864.3. [F4KF27-2]
DR RefSeq; NP_851270.1; NM_180939.2. [F4KF27-2]
DR AlphaFoldDB; F4KF27; -.
DR IntAct; F4KF27; 1.
DR STRING; 3702.AT5G64680.3; -.
DR iPTMnet; F4KF27; -.
DR PaxDb; F4KF27; -.
DR PRIDE; F4KF27; -.
DR ProteomicsDB; 250837; -. [F4KF27-1]
DR EnsemblPlants; AT5G64680.1; AT5G64680.1; AT5G64680. [F4KF27-2]
DR EnsemblPlants; AT5G64680.2; AT5G64680.2; AT5G64680. [F4KF27-2]
DR EnsemblPlants; AT5G64680.3; AT5G64680.3; AT5G64680. [F4KF27-1]
DR GeneID; 836589; -.
DR Gramene; AT5G64680.1; AT5G64680.1; AT5G64680. [F4KF27-2]
DR Gramene; AT5G64680.2; AT5G64680.2; AT5G64680. [F4KF27-2]
DR Gramene; AT5G64680.3; AT5G64680.3; AT5G64680. [F4KF27-1]
DR KEGG; ath:AT5G64680; -.
DR Araport; AT5G64680; -.
DR TAIR; locus:2174834; AT5G64680.
DR eggNOG; ENOG502RZN5; Eukaryota.
DR HOGENOM; CLU_070733_1_1_1; -.
DR InParanoid; F4KF27; -.
DR OMA; NSHEGKP; -.
DR OrthoDB; 1407483at2759; -.
DR PRO; PR:F4KF27; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; F4KF27; baseline and differential.
DR Genevisible; F4KF27; AT.
DR GO; GO:0016592; C:mediator complex; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR InterPro; IPR038823; MED2_plant.
DR PANTHER; PTHR36407; PTHR36407; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..211
FT /note="Mediator-associated protein 2"
FT /id="PRO_0000419194"
FT REGION 128..211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 173
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT VAR_SEQ 66..73
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074,
FT ECO:0000303|PubMed:14593172"
FT /id="VSP_044126"
SQ SEQUENCE 211 AA; 23328 MW; FFCAB7DDB9A4B760 CRC64;
MDFDFKVSGD FIVSGAEQLD DTDLTRSDEF WLIQAPLGQF PEIEENTLKI EPDKDGLFGE
FKDSNAGSLH LCLGAKYDLA SFHSQDAGAE LIIPSEESMI VGKITRRVAL VRYPEPNELL
QKMKARTQQK LVGSVTNSSK KSSNLTQSSR HKSGTRSSRE KSMFSGFTET PKSPKRKNSE
SSSGKHRSST STVSGSSERS AKSKKKVKKE E