ID   MDE2_SCHPO              Reviewed;         278 AA.
AC   P87309;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 3.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Mei4-dependent protein 2;
GN   Name=mde2 {ECO:0000303|PubMed:10747048};
GN   ORFNames=SPBC31F10.08 {ECO:0000312|PomBase:SPBC31F10.08};
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   INDUCTION.
RX   PubMed=10747048; DOI=10.1093/genetics/154.4.1497;
RA   Abe H., Shimoda C.;
RT   "Autoregulated expression of Schizosaccharomyces pombe meiosis-specific
RT   transcription factor Mei4 and a genome-wide search for its target genes.";
RL   Genetics 154:1497-1508(2000).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=16169489; DOI=10.1016/j.cub.2005.07.059;
RA   Gregan J., Rabitsch P.K., Sakem B., Csutak O., Latypov V., Lehmann E.,
RA   Kohli J., Nasmyth K.;
RT   "Novel genes required for meiotic chromosome segregation are identified by
RT   a high-throughput knockout screen in fission yeast.";
RL   Curr. Biol. 15:1663-1669(2005).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH REC14 AND REC15.
RX   PubMed=22841486; DOI=10.1016/j.molcel.2012.06.023;
RA   Miyoshi T., Ito M., Kugou K., Yamada S., Furuichi M., Oda A., Yamada T.,
RA   Hirota K., Masai H., Ohta K.;
RT   "A central coupler for recombination initiation linking chromosome
RT   architecture to S phase checkpoint.";
RL   Mol. Cell 47:722-733(2012).
RN   [6]
RP   INTERACTION WITH REC15.
RX   PubMed=31665745; DOI=10.1093/nar/gkz754;
RA   Kariyazono R., Oda A., Yamada T., Ohta K.;
RT   "Conserved HORMA domain-containing protein Hop1 stabilizes interaction
RT   between proteins of meiotic DNA break hotspots and chromosome axis.";
RL   Nucleic Acids Res. 47:10166-10180(2019).
CC   -!- FUNCTION: Required for the formation of meiotic double-strand breaks
CC       and for the segregation of homologous centromeres during meiosis I
CC       (PubMed:16169489). Interacts with the SFT and DSBC complexes, two
CC       subcomplexes involved in the formation of meiotic double-strand breaks.
CC       Stabilizes the SFT subcomplex on DSB sites and recruits the DSBC
CC       subcomplex onto DSB sites (PubMed:22841486).
CC       {ECO:0000269|PubMed:16169489, ECO:0000269|PubMed:22841486}.
CC   -!- SUBUNIT: Interacts with rec15; the interaction is direct
CC       (PubMed:31665745, PubMed:22841486). Interacts with the DSBC complex
CC       component rec14 (PubMed:22841486). {ECO:0000269|PubMed:22841486,
CC       ECO:0000269|PubMed:31665745}.
CC   -!- INDUCTION: Induced by transcription factor mei4.
CC       {ECO:0000269|PubMed:10747048}.
CC   -!- DISRUPTION PHENOTYPE: Causes severe chromosome missegregation without
CC       greatly affecting sporulation itself. Reduces recombination by 16- to
CC       76-fold. {ECO:0000269|PubMed:16169489}.
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DR   EMBL; CU329671; CAB10085.3; -; Genomic_DNA.
DR   PIR; T40211; T40211.
DR   RefSeq; NP_596570.2; NM_001022491.2.
DR   AlphaFoldDB; P87309; -.
DR   SMR; P87309; -.
DR   BioGRID; 276842; 7.
DR   STRING; 4896.SPBC31F10.08.1; -.
DR   PaxDb; P87309; -.
DR   PRIDE; P87309; -.
DR   EnsemblFungi; SPBC31F10.08.1; SPBC31F10.08.1:pep; SPBC31F10.08.
DR   GeneID; 2540312; -.
DR   KEGG; spo:SPBC31F10.08; -.
DR   PomBase; SPBC31F10.08; mde2.
DR   VEuPathDB; FungiDB:SPBC31F10.08; -.
DR   HOGENOM; CLU_966946_0_0_1; -.
DR   PRO; PR:P87309; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0010780; P:meiotic DNA double-strand break formation involved in reciprocal meiotic recombination; IMP:PomBase.
PE   1: Evidence at protein level;
KW   Meiosis; Reference proteome.
FT   CHAIN           1..278
FT                   /note="Mei4-dependent protein 2"
FT                   /id="PRO_0000096322"
SQ   SEQUENCE   278 AA;  31804 MW;  55EC83D9D95064E3 CRC64;
     MENYTKSTTM CGKRKPTFEI YRESDFSKIT NQGTVTAFDI ISNKETFSES KQLKKAKSRN
     VTLVDKNTKA DKRYKSEKLS DYLLNQEHHK NSPKIINDVL VIGKSDGLIN SFPLSQNYIS
     SSESQTSSGN VDIIGNDNSH ASPYLPKFEL KSQSQSNHSN VVKNQPIKLC KKTEGGTNLD
     HNKSINGAVK SGTSKSANKD RIYVFYEDEE VMEYRYIFGS RMQTKNHDIN ANNKRKIFYQ
     NSFSNFHLLH KIIQPNYEPA SAEERKLCIE ILDEVLQY