MDFA_EDWI9
ID MDFA_EDWI9 Reviewed; 410 AA.
AC C5BC70;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 2.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Multidrug transporter MdfA;
GN Name=mdfA; OrderedLocusNames=NT01EI_3857;
OS Edwardsiella ictaluri (strain 93-146).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Hafniaceae; Edwardsiella.
OX NCBI_TaxID=634503;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=93-146;
RA Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C.,
RA Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L.;
RT "Complete genome sequence of Edwardsiella ictaluri 93-146.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Efflux pump driven by the proton motive force. Confers
CC resistance to a broad spectrum of chemically unrelated drugs (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. MdfA family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP001600; ACR70976.2; -; Genomic_DNA.
DR RefSeq; WP_015873007.1; NC_012779.2.
DR AlphaFoldDB; C5BC70; -.
DR SMR; C5BC70; -.
DR STRING; 67780.B6E78_10830; -.
DR EnsemblBacteria; ACR70976; ACR70976; NT01EI_3857.
DR GeneID; 7959898; -.
DR KEGG; eic:NT01EI_3857; -.
DR PATRIC; fig|634503.3.peg.3439; -.
DR HOGENOM; CLU_001265_47_2_6; -.
DR OrthoDB; 1452500at2; -.
DR Proteomes; UP000001485; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..410
FT /note="Multidrug transporter MdfA"
FT /id="PRO_0000405335"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..52
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..109
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 131..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..255
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..313
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 314..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 335..346
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 368..375
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..410
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 410 AA; 44526 MW; 8BCFE7081CC2E5EE CRC64;
MQNHLSSTRR LGRRALLFPL CLVLYEFATY IGNDMIQPGM LSVVQTFGVD ESWVPTSMTA
YLAGGMFLQW LLGPLSDRIG RRPVMLIGTL YFAATCLAIL LTNSIEQFTL MRFLQGISLC
FIGAVGYAAI QESFEESVCI KITALMANVA LIAPLLGPLA GAAWVHLFPW EGMFILFAAL
SLLAFLGLYK AMPETATRRG EKLSLSALGR DYTLVLKNRR FLCGSLACGF ASLPLLAWIA
QSPVIIISGE GLSSYDYGML QVPIFGMLIL GNLTLARLSG RRPVRRLIQL GAWPMVGGLA
IAAASTLYSA HAYLWMTAGL SLYAFGIGLA NAGLYRLTLF SSTMSKGTVS AAMGMISMFI
YTLGIEVGKY AWLLGGNGAF NLFNLISGLL WLALIARMLR DQLVGRMAGR
