MDFA_YERPD
ID MDFA_YERPD Reviewed; 409 AA.
AC D0JKF6;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-MAR-2011, sequence version 2.
DT 25-MAY-2022, entry version 46.
DE RecName: Full=Multidrug transporter MdfA;
GN Name=mdfA; OrderedLocusNames=YPD4_3562;
OS Yersinia pestis (strain D106004).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=637382;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=D106004;
RX PubMed=19815893; DOI=10.4269/ajtmh.2009.09-0174;
RA Zhang Z., Hai R., Song Z., Xia L., Liang Y., Cai H., Liang Y., Shen X.,
RA Zhang E., Xu J., Yu D., Yu X.J.;
RT "Spatial variation of Yersinia pestis from Yunnan Province of China.";
RL Am. J. Trop. Med. Hyg. 81:714-717(2009).
CC -!- FUNCTION: Efflux pump driven by the proton motive force. Confers
CC resistance to a broad spectrum of chemically unrelated drugs (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. MdfA family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACY60466.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP001585; ACY60466.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_002215753.1; NC_017154.1.
DR AlphaFoldDB; D0JKF6; -.
DR SMR; D0JKF6; -.
DR GeneID; 66843644; -.
DR KEGG; ypd:YPD4_3562; -.
DR PATRIC; fig|637382.3.peg.4723; -.
DR HOGENOM; CLU_001265_47_2_6; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..409
FT /note="Multidrug transporter MdfA"
FT /id="PRO_0000405339"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..52
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..107
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 129..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..168
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..226
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 248..254
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 276..286
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 287..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 308..314
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 336..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..378
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 379..399
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 400..409
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 409 AA; 44065 MW; 7794BAC70DFD1947 CRC64;
MQTSFSPATR LGRRALLFPL CLVLFEFAAY IANDMIQPGM LAVVAEFNAS VEWVPTSMTA
YLAGGMFLQW LLGPLSDRRG RRPVMLAGVA FFVVTCLAIL LVNSIEQFIA MRFLQGIGLC
FIGAVGYATI QESFEEAVCI KITALMANVA LIAPLLGPLA GAALIHVAPW QTMFVLFAVL
GAISFAGLWR AMPETASLKG EKLSVANMWR DYKQVLANRR FLCGSLALGF ASLPLLAWIA
QSPVILISGE QLSTFEYGIL QVPIFGALII GNLTLARLSG KTSIPQLIRY GAGPMIVGLM
IAAGSTLYSS HAYLWMTAGL SLYAFGIGLA NAGLVRLTLF ASDISKGTVS AAMGMISMMI
FTLGIELAKV AYLWGDSRGF NLFNLMSGLL WLGLVMVFIR RQPEAVATE