MDFIC_MOUSE
ID MDFIC_MOUSE Reviewed; 247 AA.
AC Q8BX65; Q3T9H3; Q6NXM1; Q8BJS3;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=MyoD family inhibitor domain-containing protein;
DE AltName: Full=I-mfa domain-containing protein;
DE AltName: Full=Kidney cell line-derived transcript 1;
GN Name=Mdfic {ECO:0000312|MGI:MGI:104611};
GN Synonyms=Kdt1 {ECO:0000312|MGI:MGI:104611};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Aorta, Cerebellum, Eye, Spleen, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAH67006.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J {ECO:0000312|EMBL:AAH67006.1};
RC TISSUE=Brain {ECO:0000312|EMBL:AAH67006.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, INTERACTION WITH ZIC2, AND SUBCELLULAR LOCATION.
RX PubMed=15207726; DOI=10.1016/j.bbrc.2004.05.158;
RA Mizugishi K., Hatayama M., Tohmonda T., Ogawa M., Inoue T., Mikoshiba K.,
RA Aruga J.;
RT "Myogenic repressor I-mfa interferes with the function of Zic family
RT proteins.";
RL Biochem. Biophys. Res. Commun. 320:233-240(2004).
RN [4]
RP FUNCTION, INTERACTION WITH ZIC2, AND SUBCELLULAR LOCATION.
RX PubMed=15465018; DOI=10.1016/j.bbrc.2004.09.052;
RA Ishiguro A., Inoue T., Mikoshiba K., Aruga J.;
RT "Molecular properties of Zic4 and Zic5 proteins: functional diversity
RT within Zic family.";
RL Biochem. Biophys. Res. Commun. 324:302-307(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH HAND1.
RX PubMed=17891141; DOI=10.1038/ncb1633;
RA Martindill D.M.J., Risebro C.A., Smart N., Franco-Viseras Mdel M.,
RA Rosario C.O., Swallow C.J., Dennis J.W., Riley P.R.;
RT "Nucleolar release of Hand1 acts as a molecular switch to determine cell
RT fate.";
RL Nat. Cell Biol. 9:1131-1141(2007).
CC -!- FUNCTION: Acts as a transcriptional activator or repressor. Inhibits
CC the transcriptional activation of Zic family proteins ZIC1, ZIC2 and
CC ZIC3. Retains nuclear Zic proteins ZIC1, ZIC2 and ZIC3 in the
CC cytoplasm. Modulates the expression from cellular promoters. Binds to
CC the axin complex, resulting in an increase in the level of free beta-
CC catenin. Affects axin-regulation of the WNT and JNK signaling pathways.
CC {ECO:0000269|PubMed:15207726, ECO:0000269|PubMed:15465018}.
CC -!- SUBUNIT: Interacts with HAND1; leading to sequester HAND1 into the
CC nucleolus and prevent its activity (PubMed:17891141). Interacts with
CC ZIC2 (PubMed:15207726, PubMed:15465018). The C-terminus interacts with
CC AXIN1, the histidine-rich region of CCNT1/cyclin-T and weakly with
CC LEF1. Interacts with CCNT2 (By similarity).
CC {ECO:0000250|UniProtKB:Q9P1T7, ECO:0000269|PubMed:15207726,
CC ECO:0000269|PubMed:15465018, ECO:0000269|PubMed:17891141}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Nucleus, nucleolus
CC {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:16141072};
CC IsoId=Q8BX65-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|PubMed:16141072};
CC IsoId=Q8BX65-2; Sequence=VSP_052336, VSP_052337;
CC -!- DOMAIN: The cysteine-rich C-terminus is involved in its granular
CC distribution in the cytoplasm (By similarity). The C2H2-type 3, 4 and 5
CC zinc finger domains are necessary for transcription activation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MDFI family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH67006.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAC37840.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE43047.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK048821; BAC33469.1; -; mRNA.
DR EMBL; AK080178; BAC37840.1; ALT_INIT; mRNA.
DR EMBL; AK165016; BAE38001.1; -; mRNA.
DR EMBL; AK172522; BAE43047.1; ALT_INIT; mRNA.
DR EMBL; BC067006; AAH67006.1; ALT_INIT; mRNA.
DR CCDS; CCDS19919.1; -. [Q8BX65-1]
DR RefSeq; NP_780297.3; NM_175088.5. [Q8BX65-1]
DR AlphaFoldDB; Q8BX65; -.
DR IntAct; Q8BX65; 1.
DR STRING; 10090.ENSMUSP00000099186; -.
DR iPTMnet; Q8BX65; -.
DR PhosphoSitePlus; Q8BX65; -.
DR SwissPalm; Q8BX65; -.
DR jPOST; Q8BX65; -.
DR MaxQB; Q8BX65; -.
DR PaxDb; Q8BX65; -.
DR PRIDE; Q8BX65; -.
DR ProteomicsDB; 252758; -. [Q8BX65-1]
DR ProteomicsDB; 252759; -. [Q8BX65-2]
DR Antibodypedia; 31567; 158 antibodies from 24 providers.
DR DNASU; 16543; -.
DR Ensembl; ENSMUST00000101663; ENSMUSP00000099186; ENSMUSG00000041390. [Q8BX65-1]
DR Ensembl; ENSMUST00000120512; ENSMUSP00000113050; ENSMUSG00000041390. [Q8BX65-1]
DR Ensembl; ENSMUST00000189359; ENSMUSP00000140208; ENSMUSG00000041390. [Q8BX65-1]
DR GeneID; 16543; -.
DR KEGG; mmu:16543; -.
DR UCSC; uc009azc.2; mouse. [Q8BX65-1]
DR UCSC; uc012eif.1; mouse. [Q8BX65-2]
DR CTD; 29969; -.
DR MGI; MGI:104611; Mdfic.
DR VEuPathDB; HostDB:ENSMUSG00000041390; -.
DR eggNOG; ENOG502QSK8; Eukaryota.
DR GeneTree; ENSGT00940000158685; -.
DR HOGENOM; CLU_067479_0_1_1; -.
DR InParanoid; Q8BX65; -.
DR OMA; NEDCCVH; -.
DR OrthoDB; 1487193at2759; -.
DR PhylomeDB; Q8BX65; -.
DR TreeFam; TF332113; -.
DR BioGRID-ORCS; 16543; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Mdfic; mouse.
DR PRO; PR:Q8BX65; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8BX65; protein.
DR Bgee; ENSMUSG00000041390; Expressed in choroid plexus epithelium and 224 other tissues.
DR ExpressionAtlas; Q8BX65; baseline and differential.
DR Genevisible; Q8BX65; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005730; C:nucleolus; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030332; F:cyclin binding; ISS:UniProtKB.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; ISS:UniProtKB.
DR GO; GO:0030957; F:Tat protein binding; ISS:UniProtKB.
DR GO; GO:0042308; P:negative regulation of protein import into nucleus; IDA:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0050434; P:positive regulation of viral transcription; ISS:UniProtKB.
DR GO; GO:0046328; P:regulation of JNK cascade; ISO:MGI.
DR GO; GO:0030111; P:regulation of Wnt signaling pathway; ISO:MGI.
DR InterPro; IPR026134; MDFI/MDFIC.
DR PANTHER; PTHR15304; PTHR15304; 1.
DR Pfam; PF15316; MDFI; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..247
FT /note="MyoD family inhibitor domain-containing protein"
FT /id="PRO_0000280223"
FT DOMAIN 74..247
FT /note="MDFI"
FT REGION 1..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 80..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 34..48
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P1T7"
FT MOD_RES 141
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9P1T7"
FT VAR_SEQ 32..72
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_052336"
FT VAR_SEQ 73
FT /note="T -> A (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_052337"
SQ SEQUENCE 247 AA; 26094 MW; B4FBA7F07565E3B2 CRC64;
MSCAGEALAP GPAEQQCPVE AGGGRLGSPA HEACNEDNTE KDKRPATSGH TRCGLMRDQS
IWPNPSAGEL VRTQPERLPQ LQTSAQEPGK EETGKIKNGG HTRMSNGNGI PHGAKHVSVE
NHKISAPVSQ KMHRKIQSSL SVNNDISKKS KVNAVFSPKA ASSPEDCCVH CILACLFCEF
LTLCNIVLGQ ASCGICTSEA CCCCCGDEMG DDCSCPCDMD CGIMDACCES SDCLEICMEC
CGICFPS
